NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15435R-1

Symbol CG15435 Full Name CG15435

CG No CG15435 Old CG No CG15435

Synonyms CG15435

Accession No (Link to NCBI) NM_134994.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCGCAGACTACGAGATATTC

Primer Seq. 3'

aatctagaggtaccTTGTGAGGCTGCTGCTTGTTG

Predicted Fragment Size

IR fragment full Seq

0001 CCCGCAGACT ACGAGATATT CGCCAAGATT CCCACGTATT TGCACGGCTC CACAAACGAG 
0061 TTTCTCAACT GGCAAAAGCC CATTAACGTT CTGCTGGAGG AGACGACGGG CCTGAAAAAC 
0121 TCCACGGACG ACGGACTGCC ACGCAACATA TGTGCGCCGT GCATCTCCTA TCTCAAGCAT 
0181 GCCGTCACAT TCCGGGAACA GTGCATCAAG AACGCGCTGA GTCTCAAGCT GGCGGAGCTC 
0241 TACCAGCAGA AGAGGGTCAA CGTTTCGGAT GCGAACAAGA TGGACAAGGA GAGCGCCCTC 
0301 TTCACGGCGG AGGATCTGAA TTATGTGGAG CAGAGGCCAG TGCATAATCT TCTGCTAAAC 
0361 AACAGCGCCA ACAAACTGGA GTCATCCAAG GACAAGGTAC ACAAGCGACT GCTCAATCAG 
0421 ACGACTACCC AGCTAGACGG CAATGCTGAG CAGAAGATCC AGTACTTAAA CGTGCTGTTC 
0481 AACAAGCAGC AGCCTCACAA

in silico PCR Fragment

0001 CCCGCAGACT ACGAGATATT CGCCAAGATT CCCACGTATT TGCACGGCTC CACAAACGAG 
0061 TTTCTCAACT GGCAAAAGCC CATAAACGTT CTGCTGGAGG AGACGACGGG CCTGAAAAAC 
0121 TCCACGGACG ACGGACTGCC ACGCAACATA TGTGCGCCGT GCATCTCCTA TCTCAAGCAT 
0181 GCCGTCACAT TCCGGGAACA GTGCATCAAG AACGCGCTGA GTCTCAAGCT GGCGGAGCTC 
0241 TACCAGCAGA AGAGGGTCAA CGTTTCGGAT GCGAACAAGA TGGACAAGGA GAGCGCCCTC 
0301 TTCACGGCGG AGGATCTGAA TTATGTGGAG CAGAGGCCAG TGCATAATCT TCTGCTAAAC 
0361 AACAGCGCCA ACAAACTGGA GTCATCCAAG GACAAGGTAC ACAAGCGACT GCTCAATCAG 
0421 ACGACTACCC AGCTAGACGG CAATGCTGAG CAGAAGATCC AGTACTTAAA CGTGCTGTTC 
0481 AACAAGCAGC AGCCTCACAA

Assemble Data


15435R-1.IR_full       1   CCCGCAGACTACGAGATATTCGCCAAGATTCCCACGTATTTGCACGGCTCCACAAACGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     1   CCCGCAGACTACGAGATATTCGCCAAGATTCCCACGTATTTGCACGGCTCCACAAACGAG 60


15435R-1.IR_full       61  TTTCTCAACTGGCAAAAGCCCATTAACGTTCTGCTGGAGGAGACGACGGGCCTGAAAAAC 120
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
15435R-1.in silico     61  TTTCTCAACTGGCAAAAGCCCATAAACGTTCTGCTGGAGGAGACGACGGGCCTGAAAAAC 120


15435R-1.IR_full       121 TCCACGGACGACGGACTGCCACGCAACATATGTGCGCCGTGCATCTCCTATCTCAAGCAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     121 TCCACGGACGACGGACTGCCACGCAACATATGTGCGCCGTGCATCTCCTATCTCAAGCAT 180


15435R-1.IR_full       181 GCCGTCACATTCCGGGAACAGTGCATCAAGAACGCGCTGAGTCTCAAGCTGGCGGAGCTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     181 GCCGTCACATTCCGGGAACAGTGCATCAAGAACGCGCTGAGTCTCAAGCTGGCGGAGCTC 240


15435R-1.IR_full       241 TACCAGCAGAAGAGGGTCAACGTTTCGGATGCGAACAAGATGGACAAGGAGAGCGCCCTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     241 TACCAGCAGAAGAGGGTCAACGTTTCGGATGCGAACAAGATGGACAAGGAGAGCGCCCTC 300


15435R-1.IR_full       301 TTCACGGCGGAGGATCTGAATTATGTGGAGCAGAGGCCAGTGCATAATCTTCTGCTAAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     301 TTCACGGCGGAGGATCTGAATTATGTGGAGCAGAGGCCAGTGCATAATCTTCTGCTAAAC 360


15435R-1.IR_full       361 AACAGCGCCAACAAACTGGAGTCATCCAAGGACAAGGTACACAAGCGACTGCTCAATCAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     361 AACAGCGCCAACAAACTGGAGTCATCCAAGGACAAGGTACACAAGCGACTGCTCAATCAG 420


15435R-1.IR_full       421 ACGACTACCCAGCTAGACGGCAATGCTGAGCAGAAGATCCAGTACTTAAACGTGCTGTTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15435R-1.in silico     421 ACGACTACCCAGCTAGACGGCAATGCTGAGCAGAAGATCCAGTACTTAAACGTGCTGTTC 480


15435R-1.IR_full       481 AACAAGCAGCAGCCTCACAA 500
                           ||||||||||||||||||||
15435R-1.in silico     481 AACAAGCAGCAGCCTCACAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_134994.1	CG15435-RA (CG15435), mRNA
0	0	4	3	2	NM_136405.2	CG3194-RA (CG3194), mRNA
0	0	2	2	5	NM_001043220.1	CG34127-RA (CG34127), mRNA
0	0	1	6	5	NM_132025.2	CG3125-RA, transcript variant A (l(1)G0060), mRNA
0	0	1	6	5	NM_167047.1	CG3125-RB, transcript variant B (l(1)G0060), mRNA
0	0	1	5	8	NM_206236.1	CG15804-RB, transcript variant B (Dhc62B), mRNA
0	0	1	5	8	NM_057737.2	CG15804-RA, transcript variant A (Dhc62B), mRNA
0	0	1	4	7	NM_136155.2	CG10628-RA (CG10628), mRNA
0	0	1	4	7	NM_133089.2	CG6659-RA, transcript variant A (CG6659), mRNA
0	0	1	4	7	NM_167627.1	CG6659-RB, transcript variant B (CG6659), mRNA
0	0	1	3	3	NM_141173.1	CG11133-RA (CG11133), mRNA
0	0	1	3	3	NM_139532.1	CG14967-RA (CG14967), mRNA
0	0	1	3	2	NM_176132.2	CG12052-RR, transcript variant R (lola), mRNA
0	0	1	3	2	NM_080027.4	CG12052-RG, transcript variant G (lola), mRNA
0	0	1	3	2	NM_142972.3	CG6204-RA (CG6204), mRNA
0	0	1	2	4	NM_136508.2	CG8711-RA (cul-4), mRNA
0	0	1	2	3	NM_057492.3	CG4114-RA (ex), mRNA
0	0	1	2	3	NM_080046.2	CG10986-RB (g), mRNA
0	0	1	2	2	NM_137163.3	CG10255-RA (Lap1), mRNA
0	0	1	2	2	NM_140812.2	CG3902-RA (CG3902), mRNA
0	0	0	5	8	NM_136477.2	CG30497-RA, transcript variant A (CG30497), mRNA
0	0	0	5	6	NM_165555.1	CG30497-RB, transcript variant B (CG30497), mRNA
0	0	0	5	5	NM_165556.1	CG30497-RC, transcript variant C (CG30497), mRNA
0	0	0	5	3	NM_132212.2	CG1575-RA (CG1575), mRNA
0	0	0	4	12	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	0	4	5	NM_132543.1	CG10362-RA (CG10362), mRNA
0	0	0	4	4	NM_057410.3	CG10079-RA, transcript variant A (Egfr), mRNA
0	0	0	4	4	NM_057411.3	CG10079-RB, transcript variant B (Egfr), mRNA
0	0	0	4	4	NM_140606.1	CG13046-RA (CG13046), mRNA
0	0	0	4	3	NM_168784.1	CG32204-RA (CG32204), mRNA

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