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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 15408R-2 
 Symbol CG15408  Full Name CG15408 
 CG No CG15408  Old CG No CG15408 
 Synonyms CG15408 
 Accession No (Link to NCBI) NM_134923.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCGAACTGCCTGCTTAATCGC 
 Primer Seq. 3' 
aatctagaggtaccGGCAAGTGGGTGCCGATGATA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCGAACNTGC CTGCTTAATC GCAGGAATCG ATATCAGCTG CTAACCACGC TGCTGATTAA 
0061 TGTCATATCC ATTTCACACG GCATCGGTAT CGGATGGCTA TCCCCCACGC TGAGGAAACT 
0121 TCAGTCCGAC TCACCCGCGG GTTTCGAGGT GAAGTCCGAG TTCGAGATCT CCTGGGTGGG 
0181 CTCCATGCTG GGCATGGGCT CGGTGACTGG CAATATACTG ATTGGGTGCC TGCTGGGGCG 
0241 CCTGGGCAGC AAAAGGTGTC TGCTGTTAAT TGCCATACCA CATTCGTGCT TTTGGATCCT 
0301 GGTCTACTTT GCCCAGAGTG TGGAGTACCT GTACGTGGGA AGACTGTTGG CTGGCATCTG 
0361 TGGCGGCGGC ATGTACATTG TTCATCCCAT TTTACTCAGT GAAATCGCGA GATGCCAACA 
0421 TCAGAGGAAC CTTCTCTGCC ATGGTTATGC TATCGGTCAA TGTGGGAATC CTGGTGGGCT 
0481 ATATCATCGG CACCCACTTN CC 
 in silico PCR Fragment 
0001 CCGAAC-TGC CTGCTTAATC GCAGGAATCG ATATCAGCTG CTAACCACGC TGCTGATTAA 
0061 TGTCATATCC ATTTCACACG GCATCGGTAT CGGATGGCTA TCCCCCACGC TGAGGAAACT 
0121 TCAGTCCGAC TCACCCGCGG GTTTCGAGGT GAAGTCCGAG TTCGAGATCT CCTGGGTGGG 
0181 CTCCATGCTG GGCATGGGCT CGGTGACTGG CAATATACTG ATTGGGTGCC TGCTGGGGCG 
0241 CCTGGGCAGC AAAAGGTGTC TGCTGTTAAT TGCCATACCA CATTCGTGCT TTTGGATCCT 
0301 GGTCTACTTT GCCCAGAGTG TGGAGTACCT GTACGTGGGA AGACTGTTGG CTGGCATCTG 
0361 TGGCGGCGGC ATGTACATTG TTCATCCCAT TTTACTCAGT GAAATCGC-A GATGCCAACA 
0421 TCAGAGGAAC CTTCTCTGCC ATGGTTATGC TATCGGTCAA TGTGGGAATC CTGGTGGGCT 
0481 ATATCATCGG CACCCACTTG CC 
 Assemble Data 

15408R-2.IR_full       1   CCGAACNTGCCTGCTTAATCGCAGGAATCGATATCAGCTGCTAACCACGCTGCTGATTAA 60
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     1   CCGAAC-TGCCTGCTTAATCGCAGGAATCGATATCAGCTGCTAACCACGCTGCTGATTAA 60


15408R-2.IR_full       61  TGTCATATCCATTTCACACGGCATCGGTATCGGATGGCTATCCCCCACGCTGAGGAAACT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     61  TGTCATATCCATTTCACACGGCATCGGTATCGGATGGCTATCCCCCACGCTGAGGAAACT 120


15408R-2.IR_full       121 TCAGTCCGACTCACCCGCGGGTTTCGAGGTGAAGTCCGAGTTCGAGATCTCCTGGGTGGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     121 TCAGTCCGACTCACCCGCGGGTTTCGAGGTGAAGTCCGAGTTCGAGATCTCCTGGGTGGG 180


15408R-2.IR_full       181 CTCCATGCTGGGCATGGGCTCGGTGACTGGCAATATACTGATTGGGTGCCTGCTGGGGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     181 CTCCATGCTGGGCATGGGCTCGGTGACTGGCAATATACTGATTGGGTGCCTGCTGGGGCG 240


15408R-2.IR_full       241 CCTGGGCAGCAAAAGGTGTCTGCTGTTAATTGCCATACCACATTCGTGCTTTTGGATCCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     241 CCTGGGCAGCAAAAGGTGTCTGCTGTTAATTGCCATACCACATTCGTGCTTTTGGATCCT 300


15408R-2.IR_full       301 GGTCTACTTTGCCCAGAGTGTGGAGTACCTGTACGTGGGAAGACTGTTGGCTGGCATCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     301 GGTCTACTTTGCCCAGAGTGTGGAGTACCTGTACGTGGGAAGACTGTTGGCTGGCATCTG 360


15408R-2.IR_full       361 TGGCGGCGGCATGTACATTGTTCATCCCATTTTACTCAGTGAAATCGCGAGATGCCAACA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
15408R-2.in silico     361 TGGCGGCGGCATGTACATTGTTCATCCCATTTTACTCAGTGAAATCGC-AGATGCCAACA 420


15408R-2.IR_full       421 TCAGAGGAACCTTCTCTGCCATGGTTATGCTATCGGTCAATGTGGGAATCCTGGTGGGCT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15408R-2.in silico     421 TCAGAGGAACCTTCTCTGCCATGGTTATGCTATCGGTCAATGTGGGAATCCTGGTGGGCT 480


15408R-2.IR_full       481 ATATCATCGGCACCCACTTNCC 502
                           ||||||||||||||||||| ||
15408R-2.in silico     481 ATATCATCGGCACCCACTTGCC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134923.1  CG15408-RA (CG15408), mRNA 
0   0  3  6  11  NM_001043216.1  CG34113-RO, transcript variant O (CG34113), mRNA 
0   0  3  2  5  NM_131934.1  CG3626-RA (CG3626), mRNA 
0   0  2  3  2  NM_136167.1  CG13964-RA (CG13964), mRNA 
0   0  1  4  3  NM_166000.1  CG13331-RA, transcript variant A (CG13331), mRNA 
0   0  1  4  3  NM_137038.1  CG13331-RB, transcript variant B (CG13331), mRNA 
0   0  1  4  3  NM_136452.2  CG1598-RA (CG1598), mRNA 
0   0  1  3  4  NM_143668.2  CG1981-RA (Thd1), mRNA 
0   0  1  3  3  NM_136859.2  CG8991-RA (Sobp), mRNA 
0   0  1  2  5  NM_167094.1  CG4532-RE, transcript variant E (pod1), mRNA 
0   0  1  2  5  NM_132124.4  CG4532-RD, transcript variant D (pod1), mRNA 
0   0  1  2  5  NM_167093.1  CG4532-RC, transcript variant C (pod1), mRNA 
0   0  1  2  5  NM_167092.1  CG4532-RB, transcript variant B (pod1), mRNA 
0   0  1  2  5  NM_167091.1  CG4532-RA, transcript variant A (pod1), mRNA 
0   0  1  2  4  NM_135926.2  CG7653-RA (CG7653), mRNA 
0   0  1  2  3  NM_143604.1  CG11340-RA (CG11340), mRNA 
0   0  1  2  2  NM_141504.1  CG2747-RA, transcript variant A (CG2747), mRNA 
0   0  1  2  2  NM_169205.1  CG2747-RB, transcript variant B (CG2747), mRNA 
0   0  1  2  2  NM_078923.2  CG1854-RA (Or43a), mRNA 
0   0  0  6  23  NM_205899.1  CG33282-RA (CG33282), mRNA 
0   0  0  6  13  NM_134922.2  CG3285-RA (CG3285), mRNA 
0   0  0  6  7  NM_139981.2  CG6831-RA (rhea), mRNA 
0   0  0  5  3  NM_078909.2  CG12142-RA (Tsp42Eg), mRNA 
0   0  0  4  4  NM_140723.1  CG12229-RA (CG12229), mRNA 
0   0  0  4  4  NM_139551.1  CG14961-RA (CG14961), mRNA 
0   0  0  4  3  NM_138210.1  CG13894-RA (CG13894), mRNA 
0   0  0  4  2  NM_132621.1  CG12715-RA (CG12715), mRNA 
0   0  0  3  8  NM_135229.2  CG17378-RA, transcript variant A (CG17378), mRNA 
0   0  0  3  8  NM_164710.1  CG17378-RB, transcript variant B (CG17378), mRNA 
0   0  0  3  7  NM_134551.4  CG32506-RA (CG32506), mRNA 

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