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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15399R-1 |
| Symbol |
CG15399 |
Full Name |
CG15399 |
| CG No |
CG15399 |
Old CG No |
CG15399 |
| Synonyms |
CG15399 |
| Accession No (Link to NCBI) |
NM_134868.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTTGGGCGACAGAGGTGTG |
| Primer Seq. 3' |
aatctagaggtaccCGCATCAGCTTGTCCAGGTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGTTGGGCG ACAGAGGTGT GGGCAAGACT AGTTTGACCA ATTTGATGGC CACTACGGAA
0061 ATTACGCCCA CTCCAGACTC GCGAACCGTT GGCGAGGAGT CGTGGCATGT CCAGGTGCGG
0121 TTGNATGAGT ATTCCAAGCC GGTGATCCTG CCGCCCACGC CGGGGNCACC TGGACAACGC
0181 CCTCCTCGTC GGAGGACTCC GAGNAATTAC CCGTATATGC GGAGTACGCC CACCACCACG
0241 AATATCCTGT ACTTTGTCGA GTTCTACGAC CTGAACAGCG ANTTGGCGCA TGTGCCGCCA
0301 GCAGCGCGAA AGCTTCTATA AGAATATTGA CGGCATCGTC CTCGTGTACA ACATGCTGGA
0361 ACTGAGCTCC CAGGACAGCC TGCACGATTG GCTGTACGAT CCGTTGCGAC AGATCTGCAA
0421 GCATCGACAT CTGCGCATCC GTTCCATATT GAAAAACCAT AATGCGCCCA TTCTGGTGGT
0481 GGGCACCAAC CTGGACAAGC TGATGCG |
| in silico PCR Fragment |
0001 ATGTTGGGCG ACAGAGGTGT GGGCAAGACT AGTTTGACCA ATTTGATGGC CACTACGGAA
0061 ATTACGCCCA CTCCAGACTC GCGAACCGTT GGCGAGGAGT CGTGGCATGT CCAGGTGCGG
0121 TTGCATGAGT ATTCCAAGCC GGTGATCCTG CCGCCCACGC C-----CACC TGGACAACGC
0181 CCTCCTCGTC GGAGGACTCC GAG-AATTAC CCGTATATGC GGAGTACGCC CACCACCACG
0241 AATATCCTGT ACTTTGTCGA GTTCTACGAC CTGAACAGCG A-TTGGCGCA TGTGCCGCCA
0301 GCAGCGCGAA AGCTTCTATA AGAATATTGA CGGCATCGTC CTCGTGTACA ACATGCTGGA
0361 ACTGAGCTCC CAGGACAGCC TGCACGATTG GCTGTACGAT CCGTTGCGAC AGATCTGCAA
0421 GCATCGACAT CTGCGCATCC GTTCCATATT GAAAAACCAT AATGCGCCCA TTCTGGTGGT
0481 GGGCACCAAC CTGGACAAGC TGATGCG |
| Assemble Data |
15399R-1.IR_full 1 ATGTTGGGCGACAGAGGTGTGGGCAAGACTAGTTTGACCAATTTGATGGCCACTACGGAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15399R-1.in silico 1 ATGTTGGGCGACAGAGGTGTGGGCAAGACTAGTTTGACCAATTTGATGGCCACTACGGAA 60
15399R-1.IR_full 61 ATTACGCCCACTCCAGACTCGCGAACCGTTGGCGAGGAGTCGTGGCATGTCCAGGTGCGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15399R-1.in silico 61 ATTACGCCCACTCCAGACTCGCGAACCGTTGGCGAGGAGTCGTGGCATGTCCAGGTGCGG 120
15399R-1.IR_full 121 TTGNATGAGTATTCCAAGCCGGTGATCCTGCCGCCCACGCCGGGGNCACCTGGACAACGC 180
||| ||||||||||||||||||||||||||||||||||||| ||||||||||||||
15399R-1.in silico 121 TTGCATGAGTATTCCAAGCCGGTGATCCTGCCGCCCACGCC-----CACCTGGACAACGC 180
15399R-1.IR_full 181 CCTCCTCGTCGGAGGACTCCGAGNAATTACCCGTATATGCGGAGTACGCCCACCACCACG 240
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
15399R-1.in silico 181 CCTCCTCGTCGGAGGACTCCGAG-AATTACCCGTATATGCGGAGTACGCCCACCACCACG 240
15399R-1.IR_full 241 AATATCCTGTACTTTGTCGAGTTCTACGACCTGAACAGCGANTTGGCGCATGTGCCGCCA 300
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
15399R-1.in silico 241 AATATCCTGTACTTTGTCGAGTTCTACGACCTGAACAGCGA-TTGGCGCATGTGCCGCCA 300
15399R-1.IR_full 301 GCAGCGCGAAAGCTTCTATAAGAATATTGACGGCATCGTCCTCGTGTACAACATGCTGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15399R-1.in silico 301 GCAGCGCGAAAGCTTCTATAAGAATATTGACGGCATCGTCCTCGTGTACAACATGCTGGA 360
15399R-1.IR_full 361 ACTGAGCTCCCAGGACAGCCTGCACGATTGGCTGTACGATCCGTTGCGACAGATCTGCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15399R-1.in silico 361 ACTGAGCTCCCAGGACAGCCTGCACGATTGGCTGTACGATCCGTTGCGACAGATCTGCAA 420
15399R-1.IR_full 421 GCATCGACATCTGCGCATCCGTTCCATATTGAAAAACCATAATGCGCCCATTCTGGTGGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15399R-1.in silico 421 GCATCGACATCTGCGCATCCGTTCCATATTGAAAAACCATAATGCGCCCATTCTGGTGGT 480
15399R-1.IR_full 481 GGGCACCAACCTGGACAAGCTGATGCG 507
|||||||||||||||||||||||||||
15399R-1.in silico 481 GGGCACCAACCTGGACAAGCTGATGCG 507
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_134868.1 |
CG15399-RA (CG15399), mRNA |
| 0 |
0 |
5 |
4 |
2 |
NM_141353.1 |
CG15580-RA, transcript variant A (CG15580), mRNA |
| 0 |
0 |
5 |
4 |
2 |
NM_169124.1 |
CG15580-RB, transcript variant B (CG15580), mRNA |
| 0 |
0 |
4 |
6 |
4 |
NM_176575.1 |
CG33103-RA, transcript variant A (Ppn), mRNA |
| 0 |
0 |
4 |
6 |
2 |
NM_176574.1 |
CG33103-RB, transcript variant B (Ppn), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_132682.1 |
CG11162-RA (CG11162), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_167378.1 |
CG9940-RB, transcript variant B (CG9940), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_132685.1 |
CG9940-RA, transcript variant A (CG9940), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_001043222.1 |
CG9610-RC (Poxm), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_057738.3 |
CG3969-RA, transcript variant A (PR2), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_057739.3 |
CG3969-RB, transcript variant B (PR2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141559.3 |
CG8112-RA (CG8112), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206123.1 |
CG33464-RA (CG33464), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140130.1 |
CG8065-RA (CG8065), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_168232.2 |
CG32369-RA, transcript variant A (CG32369), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141297.1 |
CG2926-RA (CG2926), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_139899.3 |
CG32369-RB, transcript variant B (CG32369), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057602.3 |
CG2248-RA (Rac1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141986.2 |
CG7091-RA (CG7091), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_130639.2 |
CG2918-RA (CG2918), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166476.1 |
CG17921-RA, transcript variant A (HmgZ), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166477.1 |
CG17921-RB, transcript variant B (HmgZ), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140952.1 |
CG5585-RA (CG5585), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057981.3 |
CG4063-RA (ebi), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135401.1 |
CG9289-RA (CG9289), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165978.1 |
CG17041-RC, transcript variant C (CG17041), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165977.1 |
CG17041-RB, transcript variant B (CG17041), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137016.3 |
CG17041-RA, transcript variant A (CG17041), mRNA |
| 0 |
0 |
0 |
24 |
50 |
NM_079300.2 |
CG11720-RA (Sgs3), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_169775.1 |
CG7467-RA, transcript variant A (osa), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
 |
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