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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15393R-1 |
| Symbol |
CG15393 |
Full Name |
CG15393 |
| CG No |
CG15393 |
Old CG No |
CG15393 |
| Synonyms |
CG15393 |
| Accession No (Link to NCBI) |
NM_134841.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCCTCGTGTACAACAAGCCC |
| Primer Seq. 3' |
aatctagaggtaccGTGCTCCAGTGCGCTGGCTAT |
| Predicted Fragment Size |
357 |
| IR fragment full Seq |
0001 AGCCTCGTGT ACAACAAGCC CAGGGAGCTG GTGCCAGAGC TGCTGGAATG GGAGCTTGGC
0061 GGCGGGCAGG GGTACTCGCA TTCTAGGCAC CCATTAACAC CAACTTGGCA ACGCTTTGAT
0121 GGACAGCTGA CAGACAACCA GTCAACGGCT GAGGCGANTG ATGGCCCAGC CATCCCGANT
0181 GCCCGCTGCC TTCGGCgGCG GTGCCNACAA TTATGGGGCG ACAACTATGC GCGGCACTTG
0241 GGGACAGGCG CAGAAGCTGG CAGGGACAGC ACCCAGCAAT TAGCACTATC CCTGGCCAAC
0301 TATGAATGGA TCGAGGCGAG GCAAGTCGCT GTCGATGGCA TAGCCAGCGC ACTGGAGCAC |
| in silico PCR Fragment |
0001 AGCCTCGTGT ACAACAAGCC CAGGGAGCTG GTGCCAGAGC TGCTGGAATG GGAGCTTGGC
0061 GGCGGGCAGG GGTACTCGCA TTCTAGGCAC CCATTAACAC CAACTTGGCA ACGCTTTGAT
0121 GGACAGCTGA CAGACAACCA GTCAACGGCT GAGGCGA-TG ATGGCCCAGC CATCCCGA-T
0181 GCCCGCTGCC TTCGGCGGCG GTGCC-ACAA TTATGGGGCG ACAACTATGC GCGGCACTTG
0241 GGGACAGGCG CAGAAGCTGG CAGGGACAGC ACCCAGCAAT TAGCACTATC CCTGGCCAAC
0301 TATGAATGGA TCGAGGCGAG GCAAGTCGCT GTCGATGGCA TAGCCAGCGC ACTGGAGCAC |
| Assemble Data |
15393R-1.IR_full 1 AGCCTCGTGTACAACAAGCCCAGGGAGCTGGTGCCAGAGCTGCTGGAATGGGAGCTTGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15393R-1.in silico 1 AGCCTCGTGTACAACAAGCCCAGGGAGCTGGTGCCAGAGCTGCTGGAATGGGAGCTTGGC 60
15393R-1.IR_full 61 GGCGGGCAGGGGTACTCGCATTCTAGGCACCCATTAACACCAACTTGGCAACGCTTTGAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15393R-1.in silico 61 GGCGGGCAGGGGTACTCGCATTCTAGGCACCCATTAACACCAACTTGGCAACGCTTTGAT 120
15393R-1.IR_full 121 GGACAGCTGACAGACAACCAGTCAACGGCTGAGGCGANTGATGGCCCAGCCATCCCGANT 180
||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| |
15393R-1.in silico 121 GGACAGCTGACAGACAACCAGTCAACGGCTGAGGCGA-TGATGGCCCAGCCATCCCGA-T 180
15393R-1.IR_full 181 GCCCGCTGCCTTCGGCGGCGGTGCCNACAATTATGGGGCGACAACTATGCGCGGCACTTG 240
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
15393R-1.in silico 181 GCCCGCTGCCTTCGGCGGCGGTGCC-ACAATTATGGGGCGACAACTATGCGCGGCACTTG 240
15393R-1.IR_full 241 GGGACAGGCGCAGAAGCTGGCAGGGACAGCACCCAGCAATTAGCACTATCCCTGGCCAAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15393R-1.in silico 241 GGGACAGGCGCAGAAGCTGGCAGGGACAGCACCCAGCAATTAGCACTATCCCTGGCCAAC 300
15393R-1.IR_full 301 TATGAATGGATCGAGGCGAGGCAAGTCGCTGTCGATGGCATAGCCAGCGCACTGGAGCAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15393R-1.in silico 301 TATGAATGGATCGAGGCGAGGCAAGTCGCTGTCGATGGCATAGCCAGCGCACTGGAGCAC 360
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
339 |
0 |
0 |
0 |
NM_134841.1 |
CG15393-RA (CG15393), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_078662.2 |
CG5488-RA (B-H2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132413.1 |
CG15295-RA (CG15295), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135238.2 |
CG10354-RA (CG10354), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079207.2 |
CG4659-RA (Srp54k), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135597.2 |
CG6415-RA (CG6415), mRNA |
| 0 |
0 |
0 |
67 |
105 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167993.1 |
CG32280-RB, transcript variant B (CG32280), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167994.1 |
CG32280-RC, transcript variant C (CG32280), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_136591.3 |
CG8213-RA (CG8213), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167992.1 |
CG32280-RA, transcript variant A (CG32280), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_058151.4 |
CG6551-RA (fu), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_142221.1 |
CG18516-RA (CG18516), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132114.1 |
CG14442-RA (CG14442), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_136044.2 |
CG10333-RA (CG10333), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_206200.1 |
CG11206-RB, transcript variant B (CG11206), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137819.2 |
CG11206-RA, transcript variant A (CG11206), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135241.1 |
CG9211-RA (iHog), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001014695.1 |
CG11153-RB, transcript variant B (Sox102F), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166792.1 |
CG11153-RA, transcript variant A (Sox102F), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_169263.1 |
CG12952-RB, transcript variant B (sage), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_079563.2 |
CG12952-RA, transcript variant A (sage), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_137678.2 |
CG30295-RA (Ipk1), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_135857.1 |
CG16879-RA (CG16879), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_134960.2 |
CG2818-RA, transcript variant A (CG2818), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_166951.1 |
CG2714-RB, transcript variant B (crm), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_057757.3 |
CG2714-RA, transcript variant A (crm), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_079454.2 |
CG6948-RA (Clc), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_135181.1 |
CG13982-RA (CG13982), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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