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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15388R-4 |
| Symbol |
eys |
Full Name |
eyes shut |
| CG No |
CG33955 |
Old CG No |
CG15388 |
| Synonyms |
CG7245, CG15389, CG15388, CT35437, CG33955, eys, spam, mAb21A6, spacemaker |
| Accession No (Link to NCBI) |
NM_001032399.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACGAATGCATGTCGAATCCC |
| Primer Seq. 3' |
aatctagaggtaccTTCATGCACCGCGGTCCCAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NACGAATGCA TGTCGAATCC CTGCCAGAAC GGCGGACTCT GCCGGGACAG AACCAATGGC
0061 TACATATGCA CCTGCCAACC AGGTTACCTG GGATCTCACT GCCGAACTGG ATGTCGCTGT
0121 CTGCGAGACA GGNAACTGGT GCTCGTTGCC AGCACGGTGG TGAGTGCATC GAGGGTCCTG
0181 GACTGGAGTT CACCTGCGAC TGTCCGGCGG GATGGCATGG TCGGATTTGC CAGGAGGAGA
0241 TTAACGAGTG CGCCTCGTCG CCCTGCCAAA ATGGAGGCGT CTGCGTGGAC AAGCTGGCGG
0301 CCTATGCCTG CGCCTGTCCC ATGGGATATA CGGGCATCAA CTGCGAAGAG GAGATTCTCA
0361 TCTGTGCGGA CAATCCGTGT CAGAATAATG CCTTGTGCTT GATGGAGGAG GGTGTGCCCA
0421 CGTGCTATTG TGTACCCGAC TACCATGGCG AGAAGTGTGA GTTTCAGTAC GACGAATGCC
0481 AATTGGGACC GCGGTGCATG AA |
| in silico PCR Fragment |
0001 GACGAATGCA TGTCGAATCC CTGCCAGAAC GGCGGACTCT GCCGGGACAG AACCAATGGC
0061 TACATATGCA CCTGCCAACC AGGTTACCTG GGATCTCACT G-CGAACTGG ATGTCGCTGT
0121 CTGCGAGACA GG-AACTGGT GCTCGTTGCC AGCACGGTGG TGAGTGCATC GAGGGTCCTG
0181 GACTGGAGTT CACCTGCGAC TGTCCGGCGG GCTGGCATGG TCGGATTTGC CAGGAGGAGA
0241 TTAACGAGTG CGCCTCGTCG CCCTGCCAAA ATGGAGGCGT CTGCGTGGAC AAGCTGGCGG
0301 CCTATGCCTG CGCCTGTCCC ATGGGATATA CGGGCATCAA CTGCGAGGAG GAGATCCTCA
0361 TCTGTGCGGA CAATCCGTGT CAGAATAATG CCTTGTGCTT GATGGAGGAG GGTGTACCCA
0421 CGTGCTATTG TGTACCCGAC TATCATGGCG AGAAGTGTGA GTTCCAGTAC GACGAATGCC
0481 AATTGGGACC GCGGTGCATG AA |
| Assemble Data |
15388R-4.IR_full 1 ACGAATGCATGTCGAATCCCTGCCAGAACGGCGGACTCTGCCGGGACAGAACCAATGGC 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15388R-4.in silico 1 ACGAATGCATGTCGAATCCCTGCCAGAACGGCGGACTCTGCCGGGACAGAACCAATGGC 59
15388R-4.IR_full 61 TACATATGCACCTGCCAACCAGGTTACCTGGGATCTCACTGCCGAACTGGATGTCGCTGT 119
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
15388R-4.in silico 61 TACATATGCACCTGCCAACCAGGTTACCTGGGATCTCACTG-CGAACTGGATGTCGCTGT 119
15388R-4.IR_full 121 CTGCGAGACAGGNAACTGGTGCTCGTTGCCAGCACGGTGGTGAGTGCATCGAGGGTCCTG 179
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
15388R-4.in silico 121 CTGCGAGACAGG-AACTGGTGCTCGTTGCCAGCACGGTGGTGAGTGCATCGAGGGTCCTG 179
15388R-4.IR_full 181 GACTGGAGTTCACCTGCGACTGTCCGGCGGGATGGCATGGTCGGATTTGCCAGGAGGAGA 239
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
15388R-4.in silico 181 GACTGGAGTTCACCTGCGACTGTCCGGCGGGCTGGCATGGTCGGATTTGCCAGGAGGAGA 239
15388R-4.IR_full 241 TTAACGAGTGCGCCTCGTCGCCCTGCCAAAATGGAGGCGTCTGCGTGGACAAGCTGGCGG 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15388R-4.in silico 241 TTAACGAGTGCGCCTCGTCGCCCTGCCAAAATGGAGGCGTCTGCGTGGACAAGCTGGCGG 299
15388R-4.IR_full 301 CCTATGCCTGCGCCTGTCCCATGGGATATACGGGCATCAACTGCGAAGAGGAGATTCTCA 359
|||||||||||||||||||||||||||||||||||||||||||||| |||||||| ||||
15388R-4.in silico 301 CCTATGCCTGCGCCTGTCCCATGGGATATACGGGCATCAACTGCGAGGAGGAGATCCTCA 359
15388R-4.IR_full 361 TCTGTGCGGACAATCCGTGTCAGAATAATGCCTTGTGCTTGATGGAGGAGGGTGTGCCCA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
15388R-4.in silico 361 TCTGTGCGGACAATCCGTGTCAGAATAATGCCTTGTGCTTGATGGAGGAGGGTGTACCCA 419
15388R-4.IR_full 421 CGTGCTATTGTGTACCCGACTACCATGGCGAGAAGTGTGAGTTTCAGTACGACGAATGCC 479
|||||||||||||||||||||| |||||||||||||||||||| ||||||||||||||||
15388R-4.in silico 421 CGTGCTATTGTGTACCCGACTATCATGGCGAGAAGTGTGAGTTCCAGTACGACGAATGCC 479
15388R-4.IR_full 481 AATTGGGACCGCGGTGCATGAA 501
||||||||||||||||||||||
15388R-4.in silico 481 AATTGGGACCGCGGTGCATGAA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
11 |
NM_001032399.1 |
CG33955-RB (eys), mRNA |
| 0 |
0 |
4 |
10 |
35 |
NM_135247.2 |
CG9138-RA (SP1070), mRNA |
| 0 |
0 |
4 |
8 |
96 |
NM_057511.3 |
CG3936-RA (N), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_132719.2 |
CG32604-RA, transcript variant A (l(1)G0007), mRNA |
| 0 |
0 |
2 |
16 |
39 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0 |
0 |
2 |
16 |
39 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0 |
0 |
2 |
4 |
10 |
NM_079803.2 |
CG6127-RA (Ser), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166608.1 |
CG30184-RA (CG30184), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_078937.3 |
CG2411-RA (ptc), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_206573.1 |
CG31094-RB, transcript variant B (LpR1), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_170240.2 |
CG31094-RA, transcript variant A (LpR1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079252.2 |
CG5962-RA (Arr2), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167911.1 |
CG2086-RB, transcript variant B (drpr), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164542.3 |
CG3234-RB, transcript variant B (tim), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164540.2 |
CG3234-RD, transcript variant D (tim), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038783.1 |
CG3234-RF, transcript variant F (tim), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014463.1 |
CG3234-RE, transcript variant E (tim), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078744.3 |
CG3234-RA, transcript variant A (tim), mRNA |
| 0 |
0 |
0 |
9 |
15 |
NM_168478.1 |
CG32094-RA (CG32094), mRNA |
| 0 |
0 |
0 |
7 |
12 |
NM_132335.2 |
CG12139-RB (CG12139), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_136731.1 |
CG12908-RA, transcript variant A (Ndg), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_206074.1 |
CG12908-RB, transcript variant B (Ndg), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_169965.1 |
CG31233-RA (CG31233), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137942.1 |
CG13550-RA (CG13550), mRNA |
| 0 |
0 |
0 |
4 |
47 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
4 |
22 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_079395.2 |
CG9695-RA (Dab), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_134606.2 |
CG1486-RA, transcript variant A (CG1486), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_167743.1 |
CG1486-RB, transcript variant B (CG1486), mRNA |
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