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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 15335R-3 
 Symbol CG15335  Full Name CG15335 
 CG No CG15335  Old CG No CG15335 
 Synonyms CG15338, CG15335 
 Accession No (Link to NCBI) NM_132214.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACGTCAGCTGTGGCGTGCAAC 
 Primer Seq. 3' 
aatctagaggtaccTTGCGCCGATTCGCTGCTGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACGTCAGCTG TGGCGTGCAA CTTTGCAAGG GGAAGTGTCC GACTCTAAAT TGTCGCCTAA 
0061 AAGACGCCTC CGCCGGCGTT GAGCGCCGAG CAGCATCTGG CACGCATCGA GGTTTTCAAT 
0121 TCGCTGGCCG TGACAGCACC CCAAATCGAG GTGGACCGCC TGCGCTACGA TAGACGCCAC 
0181 AACATGAGCG GCGAGGATTA TGCGCCGCAT GTGAGGGGTC AAGTGATGCC CGGCGAGGGA 
0241 ACGCTCTGTC TGTCCATCTC CAAGCTGGCC ATCTCGTTCT GCGTTCTGGG CCTGATCTTT 
0301 CTGGTGGCCG TGGTGGTGGC CATCTTTTCG CTGATTCGAT NCGCGTCGCC GGGAGCGTCG 
0361 TCATGGAGCT GGCTTGAGTC TGGGTCTGGG AGTAGGACTG GGCATCGCCG GGACGAGCAC 
0421 GTCGAACAGC AGCAGTCGAT TGCATCGGGA TCGGGCCGAG ATTTGCACCT CGATGTTCTC 
0481 ATCCAGCAGC GAATCGGCGC AA 
 in silico PCR Fragment 
0001 ACGTCAGCTG TGGCGTGCAA CTTTGCAAGG GGAAGTGTCC GACTCTAAAT TGTCGCCT-A 
0061 AAGACGCCTC CGCCGGCGTT GAGCGCCGAG CAGCATCTGG CACGCATCGA GGTTTTCAAT 
0121 TCGCTGGCCG TGACAGCACC CCAAATCGAG GTGGACCGCC TGCGCTACGA TAGGCGGCAC 
0181 AACATGAGCG GCGAGGATTA TGCGCCGCAT GTGAGGGGTC AAGTGATGCC CGGCGAGGGA 
0241 ACGCTCTGTC TGTCCATCTC CAAGCTGGCC ATCTCGTTCT GCGTTCTGGG CCTGATCTTT 
0301 CTGGTGGCCG TGGTGGTGGC CATCTTTTCG CTGATTCGAT -CGCGTCGCC GGGAGCGTCG 
0361 TCATGGAGCT GGCTTGAGTC TGGGTCTGGG AGTAGGACTG GGCATCGCCG GGACGAGCAC 
0421 GTCGAACAGC AGCAGTCGAT TGCATCGGGA TCGGGCCGAG ATTTGCACCT CGATGTTCTC 
0481 ATCCAGCAGC GAATCGGCGC AA 
 Assemble Data 

15335R-3.IR_full       1   ACGTCAGCTGTGGCGTGCAACTTTGCAAGGGGAAGTGTCCGACTCTAAATTGTCGCCTAA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
15335R-3.in silico     1   ACGTCAGCTGTGGCGTGCAACTTTGCAAGGGGAAGTGTCCGACTCTAAATTGTCGCCT-A 60


15335R-3.IR_full       61  AAGACGCCTCCGCCGGCGTTGAGCGCCGAGCAGCATCTGGCACGCATCGAGGTTTTCAAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15335R-3.in silico     61  AAGACGCCTCCGCCGGCGTTGAGCGCCGAGCAGCATCTGGCACGCATCGAGGTTTTCAAT 120


15335R-3.IR_full       121 TCGCTGGCCGTGACAGCACCCCAAATCGAGGTGGACCGCCTGCGCTACGATAGACGCCAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| || |||
15335R-3.in silico     121 TCGCTGGCCGTGACAGCACCCCAAATCGAGGTGGACCGCCTGCGCTACGATAGGCGGCAC 180


15335R-3.IR_full       181 AACATGAGCGGCGAGGATTATGCGCCGCATGTGAGGGGTCAAGTGATGCCCGGCGAGGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15335R-3.in silico     181 AACATGAGCGGCGAGGATTATGCGCCGCATGTGAGGGGTCAAGTGATGCCCGGCGAGGGA 240


15335R-3.IR_full       241 ACGCTCTGTCTGTCCATCTCCAAGCTGGCCATCTCGTTCTGCGTTCTGGGCCTGATCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15335R-3.in silico     241 ACGCTCTGTCTGTCCATCTCCAAGCTGGCCATCTCGTTCTGCGTTCTGGGCCTGATCTTT 300


15335R-3.IR_full       301 CTGGTGGCCGTGGTGGTGGCCATCTTTTCGCTGATTCGATNCGCGTCGCCGGGAGCGTCG 360
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
15335R-3.in silico     301 CTGGTGGCCGTGGTGGTGGCCATCTTTTCGCTGATTCGAT-CGCGTCGCCGGGAGCGTCG 360


15335R-3.IR_full       361 TCATGGAGCTGGCTTGAGTCTGGGTCTGGGAGTAGGACTGGGCATCGCCGGGACGAGCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15335R-3.in silico     361 TCATGGAGCTGGCTTGAGTCTGGGTCTGGGAGTAGGACTGGGCATCGCCGGGACGAGCAC 420


15335R-3.IR_full       421 GTCGAACAGCAGCAGTCGATTGCATCGGGATCGGGCCGAGATTTGCACCTCGATGTTCTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15335R-3.in silico     421 GTCGAACAGCAGCAGTCGATTGCATCGGGATCGGGCCGAGATTTGCACCTCGATGTTCTC 480


15335R-3.IR_full       481 ATCCAGCAGCGAATCGGCGCAA 502
                           ||||||||||||||||||||||
15335R-3.in silico     481 ATCCAGCAGCGAATCGGCGCAA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  14  NM_132214.1  CG15335-RB (CG15335), mRNA 
0.41   2  2  3  2  NM_136138.2  CG10165-RA, transcript variant A (CG10165), mRNA 
0.41   2  2  3  2  NM_165309.1  CG10165-RB, transcript variant B (CG10165), mRNA 
0.2   1  3  2  2  NM_170161.1  CG31125-RA (CG31125), mRNA 
0   0  3  4  5  NM_143775.2  CG8946-RA, transcript variant A (Sply), mRNA 
0   0  3  4  5  NM_166215.1  CG8946-RB, transcript variant B (Sply), mRNA 
0   0  3  2  14  NM_057977.3  CG9239-RA, transcript variant A (B4), mRNA 
0   0  3  2  11  NM_165046.1  CG9239-RB, transcript variant B (B4), mRNA 
0   0  3  2  5  NM_130720.2  CG12632-RB (fd3F), mRNA 
0   0  3  2  2  NM_136372.2  CG3274-RA (Bap170), mRNA 
0   0  2  4  3  NM_164537.1  CG8844-RB, transcript variant B (Pdsw), mRNA 
0   0  2  4  3  NM_143715.2  CG8844-RA, transcript variant A (Pdsw), mRNA 
0   0  2  4  2  NM_170019.1  CG5315-RC, transcript variant C (CG5315), mRNA 
0   0  2  4  2  NM_142804.1  CG5315-RA, transcript variant A (CG5315), mRNA 
0   0  2  3  13  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  2  3  8  NM_001043113.1  CG33484-RC, transcript variant C (zormin), mRNA 
0   0  2  3  8  NM_206240.1  CG33484-RA, transcript variant A (zormin), mRNA 
0   0  2  3  8  NM_001043112.1  CG33484-RB, transcript variant B (zormin), mRNA 
0   0  2  3  2  NM_137681.2  CG9235-RA (CG9235), mRNA 
0   0  1  5  3  NM_176780.1  CG14616-RE, transcript variant E (l(1)G0196), mRNA 
0   0  1  5  3  NM_176779.1  CG14616-RA, transcript variant A (l(1)G0196), mRNA 
0   0  1  5  3  NM_176778.1  CG14616-RD, transcript variant D (l(1)G0196), mRNA 
0   0  1  5  3  NM_176777.1  CG14616-RC, transcript variant C (l(1)G0196), mRNA 
0   0  1  4  5  NM_141046.3  CG7605-RA (Rab26), mRNA 
0   0  1  3  8  NM_166017.1  CG18076-RE, transcript variant E (shot), mRNA 
0   0  1  3  8  NM_079009.2  CG18076-RA, transcript variant A (shot), mRNA 
0   0  1  3  8  NM_132729.1  CG9400-RA (CG9400), mRNA 
0   0  1  3  7  NM_139854.1  CG8583-RA (sec63), mRNA 
0   0  1  3  5  NM_166015.1  CG18076-RG, transcript variant G (shot), mRNA 
0   0  1  3  5  NM_166016.1  CG18076-RB, transcript variant B (shot), mRNA 

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