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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15330R-1

Symbol CG15330 Full Name CG15330

CG No CG15330 Old CG No CG15330

Synonyms CG15330

Accession No (Link to NCBI) NM_132202.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACAACACTTTGGCGCTTGCAG

Primer Seq. 3'

aatctagaggtaccTCCAGTAGCTCGGCCACAAAT

Predicted Fragment Size

IR fragment full Seq

0001 ACAACACTTT GGCGCTTGCA GCAGGCGCAT CAGCGGCAAC TTAACGAGTG GGACAGGATT 
0061 GGTAGACGCC TCAAGCGGCT GACGGGCAAG AGGTCCAGCG ACTTGCTCAT CCACGATCCG 
0121 CTCCTGCAGC CACCGTTCGT CGCCCATCCT GGCCAACGGA TGAGCGGTGG TGGTCAGCAG 
0181 ACGCCGATGC CGTTGCCAAT GGCCAGGAAG CAGACAACGC AGATCAGACG CAATCATCCG 
0241 AATAAATCGG TTAAATTCAA AGCATCCAAT AATAACACCT TGGATCTACT TGTCGTGGGT 
0301 CANACAGCTG CAAATGCATC GTCCACCTGG ACGACGGCAT GTGCAGCTCC ACAGTCCCAT 
0361 GTCCTCTCAG CCCCGCCAGC ATATCGAAGA GTATGTCTAT TTCTATCCGG TCCACTACCA 
0421 TGAGACGGAC CACTGGCCAC CACACGGCCA GCCGGAACCA AATCTTGTGG TGGATGCACG 
0481 ATTTGTGNCC GAGCTACTGG A

in silico PCR Fragment

0001 ACAACACTTT GGCGCTTGCA GCAGGCGCAT CAGCGGCAAC TTAACGAGTG GGACAGGATT 
0061 GGTAGACGCC TCAAGCGGCT GACGGGCAAG AGGTCCAGCG ACTTGCTCAT CCACGATCCG 
0121 CTCCTGCAGC CACCGTTCGT CGCCCATCCT GGCCAACGGA TGAGCGGTGG TGGTCAGCAG 
0181 ACGCCGATGC CGTTGCCAAT GGCCAGGAAG CAGACAACGC AGATCAGACG CAATCATCCG 
0241 AATAAATCGG TTAAATTCAA AGCATCCAAT AATAACACCT TGGATCTACT TGTCGTGGGT 
0301 CA-ACAGCTG CAAATGCATC GTCCACCTGG ACGACGGCAT GTGCAGCTCC ACAGTCCCAT 
0361 GTCCTCTCAG CCCCGCCAGC ATATCGAAGA GTATGTCTAT TTCTATCCGG TCCACTACCA 
0421 TGAGACGGAC CACTGGCCAC CACACGGCCA GCCGGAACCA AATCTTGTGG TGGATGCACG 
0481 ATTTGTGGCC GAGCTACTGG A

Assemble Data


15330R-1.IR_full       1   ACAACACTTTGGCGCTTGCAGCAGGCGCATCAGCGGCAACTTAACGAGTGGGACAGGATT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     1   ACAACACTTTGGCGCTTGCAGCAGGCGCATCAGCGGCAACTTAACGAGTGGGACAGGATT 60


15330R-1.IR_full       61  GGTAGACGCCTCAAGCGGCTGACGGGCAAGAGGTCCAGCGACTTGCTCATCCACGATCCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     61  GGTAGACGCCTCAAGCGGCTGACGGGCAAGAGGTCCAGCGACTTGCTCATCCACGATCCG 120


15330R-1.IR_full       121 CTCCTGCAGCCACCGTTCGTCGCCCATCCTGGCCAACGGATGAGCGGTGGTGGTCAGCAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     121 CTCCTGCAGCCACCGTTCGTCGCCCATCCTGGCCAACGGATGAGCGGTGGTGGTCAGCAG 180


15330R-1.IR_full       181 ACGCCGATGCCGTTGCCAATGGCCAGGAAGCAGACAACGCAGATCAGACGCAATCATCCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     181 ACGCCGATGCCGTTGCCAATGGCCAGGAAGCAGACAACGCAGATCAGACGCAATCATCCG 240


15330R-1.IR_full       241 AATAAATCGGTTAAATTCAAAGCATCCAATAATAACACCTTGGATCTACTTGTCGTGGGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     241 AATAAATCGGTTAAATTCAAAGCATCCAATAATAACACCTTGGATCTACTTGTCGTGGGT 300


15330R-1.IR_full       301 CANACAGCTGCAAATGCATCGTCCACCTGGACGACGGCATGTGCAGCTCCACAGTCCCAT 360
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     301 CA-ACAGCTGCAAATGCATCGTCCACCTGGACGACGGCATGTGCAGCTCCACAGTCCCAT 360


15330R-1.IR_full       361 GTCCTCTCAGCCCCGCCAGCATATCGAAGAGTATGTCTATTTCTATCCGGTCCACTACCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     361 GTCCTCTCAGCCCCGCCAGCATATCGAAGAGTATGTCTATTTCTATCCGGTCCACTACCA 420


15330R-1.IR_full       421 TGAGACGGACCACTGGCCACCACACGGCCAGCCGGAACCAAATCTTGTGGTGGATGCACG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15330R-1.in silico     421 TGAGACGGACCACTGGCCACCACACGGCCAGCCGGAACCAAATCTTGTGGTGGATGCACG 480


15330R-1.IR_full       481 ATTTGTGNCCGAGCTACTGGA 501
                           ||||||| |||||||||||||
15330R-1.in silico     481 ATTTGTGGCCGAGCTACTGGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_132202.2	CG15330-RA (CG15330), mRNA
0	0	3	5	5	NM_168162.1	CG10173-RA (Best2), mRNA
0	0	2	3	2	NM_169442.1	CG12213-RA, transcript variant A (CG12213), mRNA
0	0	2	3	2	NM_141876.1	CG12213-RB, transcript variant B (CG12213), mRNA
0	0	2	2	2	NM_165177.1	CG17332-RB, transcript variant B (VhaSFD), mRNA
0	0	2	2	2	NM_165178.1	CG17332-RD, transcript variant D (VhaSFD), mRNA
0	0	2	2	2	NM_078861.2	CG17332-RA, transcript variant A (VhaSFD), mRNA
0	0	1	6	2	NM_132797.2	CG9198-RA, transcript variant A (shtd), mRNA
0	0	1	5	4	NM_079216.3	CG10541-RA (Tektin-C), mRNA
0	0	1	4	3	NM_140769.1	CG5290-RA (CG5290), mRNA
0	0	1	3	9	NM_134495.2	CG12234-RA (Ranbp21), mRNA
0	0	1	3	9	NM_170094.2	CG13598-RA, transcript variant A (sba), mRNA
0	0	1	3	9	NM_176549.1	CG13598-RB, transcript variant B (sba), mRNA
0	0	1	3	7	NM_142538.1	CG5237-RA (CG5237), mRNA
0	0	1	3	6	NM_134682.2	CG4133-RA (CG4133), mRNA
0	0	1	3	5	NM_136921.1	CG13162-RA (CG13162), mRNA
0	0	1	2	7	NM_140424.1	CG9007-RA (CG9007), mRNA
0	0	1	2	3	NM_166411.1	CG8994-RB, transcript variant B (exu), mRNA
0	0	1	2	3	NM_166623.1	CG4051-RA (egl), mRNA
0	0	1	2	3	NM_166410.1	CG8994-RA, transcript variant A (exu), mRNA
0	0	1	2	3	NM_166412.1	CG8994-RC, transcript variant C (exu), mRNA
0	0	1	1	3	NM_132685.1	CG9940-RA, transcript variant A (CG9940), mRNA
0	0	1	1	3	NM_167378.1	CG9940-RB, transcript variant B (CG9940), mRNA
0	0	0	6	17	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	0	6	8	NM_142597.2	CG12254-RA (MED25), mRNA
0	0	0	5	16	NM_165853.1	CG13188-RA, transcript variant A (CG13188), mRNA
0	0	0	5	8	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
0	0	0	5	8	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
0	0	0	5	8	NM_080105.2	CG31243-RF, transcript variant F (cpo), mRNA

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