NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 15314R-1

Symbol CG15314 Full Name CG15314

CG No CG15314 Old CG No CG15314

Synonyms CG15314

Accession No (Link to NCBI) NM_132371.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTACCGCGAACGAACCACT

Primer Seq. 3'

aatctagaggtaccTTGTCAGCCGACGCGTTTGAA

Predicted Fragment Size

IR fragment full Seq

0001 ATGTACCGCG AACGAACCAC TTTTGACTAC CGACTGGCAG CTGCGACAAC CAACGACTGG 
0061 CAGCGTTTCG CGGCGACCGG TGTTGAGTGT CCGCCGGCGG ATTATCAACT CGCCGACGTC 
0121 GGCAGCGGTT GAAACCACCA CGCAGCAGCC AGCAGCAGAA CCACCGACGA CCTCCAAATA 
0181 CAGCCGACTG CGCAGCAGAC NCCTCCGCAA CAGCAACAGC AGCAGCGGCA GCAGCAACAA 
0241 CGACCACTGC AGCAGCAACA ACAGCTTTCC CAGCAGCAAC ATCTGCTCCC GGCGGCAGAA 
0301 CCACCAGCAA CATTTACCTG AGCAAGCTGA AAGCCAAGAG CGGTGCCGCA GCAGCAGCAG 
0361 CAGCATCCGG TGAGGCAGCA ACACTGACGC CAGCAACTAG CAACATCAGC AGCAGCAGCA 
0421 ATGACATCAC ACAAAAGCAG CACAAGTTCC AGCCCGCCAG TTTTGCTCTG CGCCGCCAAT 
0481 TTCAAACGCG TCGGCTGACA A

in silico PCR Fragment

0001 ATGTACCGCG AACGAACCAC TTTTGACTAC CGACTGGCAG CTGCGACAAC CAACGACTGG 
0061 CAGCGTTTCG CGGCGACCGG TGTTGAGTGT CCGCCGGCGG ATTATCAACT CGCCGACGTC 
0121 GGCAGCGGTT GAAACCACCA CGCAGCAGCC AGCAGCAGAA CCACCGACGA CCTCCAAATA 
0181 CAGCCGACTG CGCAGCAGAC -CCTCCGCAA CAGCAACAGC AGCAGCGGCA GCAGCAACAA 
0241 CGACCACTGC AGCAGCAACA ACAGCTTTCC CAGCAGCAAC ATCTGCTCCC GGCGGCAGAA 
0301 CCACCAGCAA CATTTACCTG AGCAAGCTGA AAGCCAAGAG CGGTGCCGCA GCAGCAGCAG 
0361 CAGCATCCGG TGAGGCAGCA ACACTGACGC CAGCAACTAG CAACATCAGC AGCAGCAGCA 
0421 ATGACATCAC ACAAAAGCAG CACAAGTTCC AGCCCGCCAG TTTTGCTCTG CGCCGCCAAT 
0481 TTCAAACGCG TCGGCTGACA A

Assemble Data


15314R-1.IR_full       1   ATGTACCGCGAACGAACCACTTTTGACTACCGACTGGCAGCTGCGACAACCAACGACTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     1   ATGTACCGCGAACGAACCACTTTTGACTACCGACTGGCAGCTGCGACAACCAACGACTGG 60


15314R-1.IR_full       61  CAGCGTTTCGCGGCGACCGGTGTTGAGTGTCCGCCGGCGGATTATCAACTCGCCGACGTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     61  CAGCGTTTCGCGGCGACCGGTGTTGAGTGTCCGCCGGCGGATTATCAACTCGCCGACGTC 120


15314R-1.IR_full       121 GGCAGCGGTTGAAACCACCACGCAGCAGCCAGCAGCAGAACCACCGACGACCTCCAAATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     121 GGCAGCGGTTGAAACCACCACGCAGCAGCCAGCAGCAGAACCACCGACGACCTCCAAATA 180


15314R-1.IR_full       181 CAGCCGACTGCGCAGCAGACNCCTCCGCAACAGCAACAGCAGCAGCGGCAGCAGCAACAA 240
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     181 CAGCCGACTGCGCAGCAGAC-CCTCCGCAACAGCAACAGCAGCAGCGGCAGCAGCAACAA 240


15314R-1.IR_full       241 CGACCACTGCAGCAGCAACAACAGCTTTCCCAGCAGCAACATCTGCTCCCGGCGGCAGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     241 CGACCACTGCAGCAGCAACAACAGCTTTCCCAGCAGCAACATCTGCTCCCGGCGGCAGAA 300


15314R-1.IR_full       301 CCACCAGCAACATTTACCTGAGCAAGCTGAAAGCCAAGAGCGGTGCCGCAGCAGCAGCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     301 CCACCAGCAACATTTACCTGAGCAAGCTGAAAGCCAAGAGCGGTGCCGCAGCAGCAGCAG 360


15314R-1.IR_full       361 CAGCATCCGGTGAGGCAGCAACACTGACGCCAGCAACTAGCAACATCAGCAGCAGCAGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     361 CAGCATCCGGTGAGGCAGCAACACTGACGCCAGCAACTAGCAACATCAGCAGCAGCAGCA 420


15314R-1.IR_full       421 ATGACATCACACAAAAGCAGCACAAGTTCCAGCCCGCCAGTTTTGCTCTGCGCCGCCAAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15314R-1.in silico     421 ATGACATCACACAAAAGCAGCACAAGTTCCAGCCCGCCAGTTTTGCTCTGCGCCGCCAAT 480


15314R-1.IR_full       481 TTCAAACGCGTCGGCTGACAA 501
                           |||||||||||||||||||||
15314R-1.in silico     481 TTCAAACGCGTCGGCTGACAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	36	142	NM_132371.1	CG15314-RA (CG15314), mRNA
1.86	9	24	132	462	NM_168361.1	CG16711-RB, transcript variant B (CG16711), mRNA
1.86	9	24	132	462	NM_140090.1	CG16711-RA, transcript variant A (CG16711), mRNA
1.65	8	36	138	371	NM_001043298.1	CG33553-RH, transcript variant H (Doa), mRNA
1.65	8	36	138	371	NM_001014681.1	CG33553-RD, transcript variant D (Doa), mRNA
1.65	8	36	137	354	NM_001014679.1	CG33553-RC, transcript variant C (Doa), mRNA
1.45	7	70	309	877	NM_132288.1	CG15365-RA (CG15365), mRNA
1.45	7	16	110	349	NM_001043260.1	CG34157-RD, transcript variant D (Dys), mRNA
1.45	7	13	131	500	NM_206622.2	CG32776-RB, transcript variant B (CG32776), mRNA
1.45	7	13	131	500	NM_001038740.1	CG32776-RD, transcript variant D (CG32776), mRNA
1.45	7	8	30	108	NM_080015.2	CG5462-RD, transcript variant D (scrib), mRNA
1.45	7	8	30	108	NM_170276.1	CG5462-RB, transcript variant B (scrib), mRNA
1.45	7	8	30	108	NM_001043296.1	CG5462-RG, transcript variant G (scrib), mRNA
1.45	7	8	30	108	NM_170275.1	CG5462-RA, transcript variant A (scrib), mRNA
1.45	7	8	30	108	NM_001014670.2	CG5462-RH, transcript variant H (scrib), mRNA
1.24	6	94	367	644	NM_079218.2	CG10491-RA, transcript variant A (vn), mRNA
1.24	6	94	367	644	NM_001014565.1	CG10491-RB, transcript variant B (vn), mRNA
1.24	6	71	355	787	NM_176592.1	CG12071-RB, transcript variant B (CG12071), mRNA
1.24	6	71	355	787	NM_143575.2	CG12071-RA, transcript variant A (CG12071), mRNA
1.24	6	59	223	619	NM_132235.2	CG32717-RB, transcript variant B (sdt), mRNA
1.24	6	59	216	558	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
1.24	6	59	216	558	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
1.24	6	59	216	558	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
1.24	6	59	216	558	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
1.24	6	43	206	469	NM_139495.1	CG9973-RA (CG9973), mRNA
1.24	6	43	202	464	NM_079902.2	CG6741-RB, transcript variant B (a), mRNA
1.24	6	43	202	464	NM_166514.1	CG6741-RA, transcript variant A (a), mRNA
1.24	6	43	202	464	NM_001014543.1	CG6741-RC, transcript variant C (a), mRNA
1.24	6	41	180	495	NM_080362.2	CG31868-RA (Samuel), mRNA
1.24	6	32	180	388	NM_168037.1	CG32264-RA, transcript variant A (CG32264), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance
+

Back