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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1527R-3 |
| Symbol |
RpS14b |
Full Name |
Ribosomal protein S14b |
| CG No |
CG1527 |
Old CG No |
CG1527 |
| Synonyms |
S14, CG1527, RpS14, RPS14B, rpS14B, anon-EST:Posey77, anon-EST:Posey154, anon-EST:Posey131, anon-EST:Posey115, RpS14B, RpS14b |
| Accession No (Link to NCBI) |
NM_080427.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTTCAGAAGGAGGAGGTTCAG |
| Primer Seq. 3' |
aatctagaggtaccCGGGTAGAGTCCGATGGGATA |
| Predicted Fragment Size |
401 |
| IR fragment full Seq |
0001 GTTCAGAAGG AGGAGGTTCA GGTCCAGCTG GGACCCCAAG TTCGCGACGG CGAGATCGTG
0061 TTCGGAGTGG CTCACATCTA CGCCAGCTTC AACGACACCT TCGTCCATGT CACGGATCTG
0121 TCCGGCCGTG AGACCATCGC TCGTGTCACC GGAGGCATGA AGGTGAAGGC CGATCGTGAT
0181 GAGGCTTCGC CCTAACGCCG CTATGTTGGC CGCTCAGGAT GTGGCTGAGA AGTGCAAGAC
0241 ANCTGGGCAT CACTGCCCTG CATATTAAGC TGCGTGCCAC CGGCGGCAAC AAGACCAAGA
0301 CCCCCGGACC CGGCGCCCAG TCCGCTCTGC GTGCGTTGGC CCGTTCGTCC ATGAAGATTG
0361 GCCGCATCGA GGATGTGACC CCTATCCCAT CGGACTCTAC CCG |
| in silico PCR Fragment |
0001 GTTCAGAAGG AGGAGGTTCA GGTCCAGCTG GGACCCCAAG TTCGCGACGG CGAGATCGTG
0061 TTCGGAGTGG CTCACATCTA CGCCAGCTTC AACGACACCT TCGTCCATGT CACGGATCTG
0121 TCCGGCCGTG AGACCATCGC TCGTGTCACC GGAGGCATGA AGGTGAAGGC CGATCGTGAT
0181 GAGGCTTCGC CCT-ACGCCG CTATGTTGGC CGCTCAGGAT GTGGCTGAGA AGTGCAAGAC
0241 A-CTGGGCAT CACTGCCCTG CATATTAAGC TGCGTGCCAC CGGCGGCAAC AAGACCAAGA
0301 CCCCCGGACC CGGCGCCCAG TCCGCTCTGC GTGCGTTGGC CCGTTCGTCC ATGAAGATTG
0361 GCCGCATCGA GGATGTGACC CCTATCCCAT CGGACTCTAC CCG |
| Assemble Data |
1527R-3.IR_full 1 GTTCAGAAGGAGGAGGTTCAGGTCCAGCTGGGACCCCAAGTTCGCGACGGCGAGATCGTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 1 GTTCAGAAGGAGGAGGTTCAGGTCCAGCTGGGACCCCAAGTTCGCGACGGCGAGATCGTG 60
1527R-3.IR_full 61 TTCGGAGTGGCTCACATCTACGCCAGCTTCAACGACACCTTCGTCCATGTCACGGATCTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 61 TTCGGAGTGGCTCACATCTACGCCAGCTTCAACGACACCTTCGTCCATGTCACGGATCTG 120
1527R-3.IR_full 121 TCCGGCCGTGAGACCATCGCTCGTGTCACCGGAGGCATGAAGGTGAAGGCCGATCGTGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 121 TCCGGCCGTGAGACCATCGCTCGTGTCACCGGAGGCATGAAGGTGAAGGCCGATCGTGAT 180
1527R-3.IR_full 181 GAGGCTTCGCCCTAACGCCGCTATGTTGGCCGCTCAGGATGTGGCTGAGAAGTGCAAGAC 240
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 181 GAGGCTTCGCCCT-ACGCCGCTATGTTGGCCGCTCAGGATGTGGCTGAGAAGTGCAAGAC 240
1527R-3.IR_full 241 ANCTGGGCATCACTGCCCTGCATATTAAGCTGCGTGCCACCGGCGGCAACAAGACCAAGA 300
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 241 A-CTGGGCATCACTGCCCTGCATATTAAGCTGCGTGCCACCGGCGGCAACAAGACCAAGA 300
1527R-3.IR_full 301 CCCCCGGACCCGGCGCCCAGTCCGCTCTGCGTGCGTTGGCCCGTTCGTCCATGAAGATTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 301 CCCCCGGACCCGGCGCCCAGTCCGCTCTGCGTGCGTTGGCCCGTTCGTCCATGAAGATTG 360
1527R-3.IR_full 361 GCCGCATCGAGGATGTGACCCCTATCCCATCGGACTCTACCCG 403
|||||||||||||||||||||||||||||||||||||||||||
1527R-3.in silico 361 GCCGCATCGAGGATGTGACCCCTATCCCATCGGACTCTACCCG 403
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
383 |
0 |
0 |
0 |
NM_080427.2 |
CG1527-RA (RpS14b), mRNA |
| 78.85 |
302 |
62 |
18 |
1 |
NM_080145.4 |
CG1524-RB, transcript variant B (RpS14a), mRNA |
| 78.85 |
302 |
62 |
18 |
1 |
NM_167137.1 |
CG1524-RA, transcript variant A (RpS14a), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_140688.2 |
CG7728-RA (CG7728), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_165249.1 |
CG5803-RB, transcript variant B (Fas3), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_165250.1 |
CG5803-RA, transcript variant A (Fas3), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169291.1 |
CG9381-RB, transcript variant B (mura), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169292.2 |
CG9381-RC, transcript variant C (mura), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_141645.2 |
CG9381-RA, transcript variant A (mura), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_176100.1 |
CG30492-RC, transcript variant C (CG30492), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_176101.1 |
CG30492-RE, transcript variant E (CG30492), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170613.2 |
CG30492-RA, transcript variant A (CG30492), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170614.2 |
CG30492-RB, transcript variant B (CG30492), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_137384.2 |
CG4878-RB, transcript variant B (eIF3-S9), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_166239.2 |
CG4878-RA, transcript variant A (eIF3-S9), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169654.2 |
CG31302-RC, transcript variant C (CG31302), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169652.2 |
CG31302-RA, transcript variant A (CG31302), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169653.2 |
CG31302-RB, transcript variant B (CG31302), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001031986.1 |
CG33719-RB, transcript variant B (Pif1A), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167027.2 |
CG6824-RC, transcript variant C (ovo), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_167026.2 |
CG6824-RB, transcript variant B (ovo), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_078603.2 |
CG10952-RA, transcript variant A (eag), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_001042810.1 |
CG10952-RB, transcript variant B (eag), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136879.2 |
CG8364-RA, transcript variant A (Rep3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136878.2 |
CG8364-RB, transcript variant B (Rep3), mRNA |
| 0 |
0 |
0 |
16 |
42 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
16 |
42 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_168738.1 |
CG32184-RA (CG32184), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
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|
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