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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15270R-2 |
| Symbol |
CG15270 |
Full Name |
CG15270 |
| CG No |
CG15270 |
Old CG No |
CG15270 |
| Synonyms |
BG:DS04929.1, CG15270 |
| Accession No (Link to NCBI) |
NM_135894.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAGGATGTACCGGACTCCAC |
| Primer Seq. 3' |
aatctagaggtaccCCCTCAAAGCACCGCCCAAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNAGNATGTA CCGGACTCCA CAGCGACAAC AACGGCGACT TCAACAGCCG GCGAGCTTGG
0061 TGGCGGCCCA AGGGATGGCG GCGAGGATGA TTTCACCGAT GACAACAACT TCGAATCGAA
0121 TTTGCGACGT CGCAAAGGCA AAATAATTTC CTGTGCCAAG GAGACCATCG AGAATGCTTC
0181 CCGCCAACTA CGCCGCAAAG TGCCCTACGC AGGTCATTTG ATGACTCCTC GTCGCCTTTG
0241 GCTCAATAAA ATTCCCACAC AATGTGATGT GGTTATTGAA TTTCCTGAGG ATGCACCAGA
0301 CGaANCNCTA CGCTgGCTCC TAGCCCGTAT CCGCTcNCAN CCGCCGGCTG GTCTGGGTCT
0361 ATTGGTCCAG GTCAAGGCGC ACGAGAGTAC GCgGNGAAAC GCGTTCTATG TCAGTGCTCC
0421 TGTTAATGTA CTGTTTAAAG CCGCTGAAGA AGCCCGCCTG CCAAAACGCC TGCGACCGGA
0481 TTTGGNCGGT GCTTTGAGGG |
| in silico PCR Fragment |
0001 GGAGGATGTA CCGGACTCCA CAGCGACAAC AACGGCGACT TCAACAGCCG GCGAGCTTGG
0061 TGGCGGCCCA AGGGATGGCG GCGAGGATGA TTTCACCGAT GACAACAACT TCGAATCGAA
0121 TTTGCGACGT CGCAAAGGCA AAATAATTTC CTGTGCCAAG GAGACCATCG AGAATGCTTC
0181 CCGCCAACTA CGCCGCAAAG TGCCCTACGC AGGTCATTTG ATGACTCCTC GTCGCCTTTG
0241 GCTCAATAAA ATTCCCACAC AATGTGATGT GGTTATTGAA TTTCCTGAGG ATGCACCAGA
0301 CGAAGCGCTA CGCTGGCTCC TAGCCCGTAT CCGCTCGCAG CCGCCGGCTG GTCTGGGTCT
0361 ATTGGTCCAG GTCAAGGCGC ACGAGAGTAC GCGGCGAAAC GCGTTCTATG TCAGTGCTCC
0421 TGTTAATGTA CTGTTTAAAG CCGCTGAAGA AGCCCGCCTG CCAAAACGCC TGCGACCGGA
0481 TTTGGGCGGT GCTTTGAGGG |
| Assemble Data |
15270R-2.IR_full 1 GNAGNATGTACCGGACTCCACAGCGACAACAACGGCGACTTCAACAGCCGGCGAGCTTGG 60
| || |||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 1 GGAGGATGTACCGGACTCCACAGCGACAACAACGGCGACTTCAACAGCCGGCGAGCTTGG 60
15270R-2.IR_full 61 TGGCGGCCCAAGGGATGGCGGCGAGGATGATTTCACCGATGACAACAACTTCGAATCGAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 61 TGGCGGCCCAAGGGATGGCGGCGAGGATGATTTCACCGATGACAACAACTTCGAATCGAA 120
15270R-2.IR_full 121 TTTGCGACGTCGCAAAGGCAAAATAATTTCCTGTGCCAAGGAGACCATCGAGAATGCTTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 121 TTTGCGACGTCGCAAAGGCAAAATAATTTCCTGTGCCAAGGAGACCATCGAGAATGCTTC 180
15270R-2.IR_full 181 CCGCCAACTACGCCGCAAAGTGCCCTACGCAGGTCATTTGATGACTCCTCGTCGCCTTTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 181 CCGCCAACTACGCCGCAAAGTGCCCTACGCAGGTCATTTGATGACTCCTCGTCGCCTTTG 240
15270R-2.IR_full 241 GCTCAATAAAATTCCCACACAATGTGATGTGGTTATTGAATTTCCTGAGGATGCACCAGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 241 GCTCAATAAAATTCCCACACAATGTGATGTGGTTATTGAATTTCCTGAGGATGCACCAGA 300
15270R-2.IR_full 301 CGAANCNCTACGCTGGCTCCTAGCCCGTATCCGCTCNCANCCGCCGGCTGGTCTGGGTCT 360
|||| | ||||||||||||||||||||||||||||| || ||||||||||||||||||||
15270R-2.in silico 301 CGAAGCGCTACGCTGGCTCCTAGCCCGTATCCGCTCGCAGCCGCCGGCTGGTCTGGGTCT 360
15270R-2.IR_full 361 ATTGGTCCAGGTCAAGGCGCACGAGAGTACGCGGNGAAACGCGTTCTATGTCAGTGCTCC 420
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
15270R-2.in silico 361 ATTGGTCCAGGTCAAGGCGCACGAGAGTACGCGGCGAAACGCGTTCTATGTCAGTGCTCC 420
15270R-2.IR_full 421 TGTTAATGTACTGTTTAAAGCCGCTGAAGAAGCCCGCCTGCCAAAACGCCTGCGACCGGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15270R-2.in silico 421 TGTTAATGTACTGTTTAAAGCCGCTGAAGAAGCCCGCCTGCCAAAACGCCTGCGACCGGA 480
15270R-2.IR_full 481 TTTGGNCGGTGCTTTGAGGG 500
||||| ||||||||||||||
15270R-2.in silico 481 TTTGGGCGGTGCTTTGAGGG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
8 |
NM_135894.2 |
CG15270-RA, transcript variant A (CG15270), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_205997.1 |
CG15270-RB, transcript variant B (CG15270), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057333.3 |
CG5711-RA (Arr1), mRNA |
| 0 |
0 |
1 |
6 |
9 |
NM_136646.1 |
CG13953-RA (CG13953), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_168681.2 |
CG3849-RB, transcript variant B (Lasp), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_140655.1 |
CG3849-RA, transcript variant A (Lasp), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136041.1 |
CG10231-RA (Pde11), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140048.2 |
CG4080-RA (CG4080), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132429.2 |
CG2202-RA (CG2202), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132120.1 |
CG14438-RA, transcript variant A (CG14438), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167090.1 |
CG14438-RB, transcript variant B (CG14438), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169576.1 |
CG31321-RB (CG31321), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001015147.1 |
CG40203-PA.3 (CG40203), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001015125.1 |
CG40235-PA.3 (CG40235), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079504.3 |
CG1133-RA (opa), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_136591.3 |
CG8213-RA (CG8213), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078772.2 |
CG13772-RA (neuroligin), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140859.1 |
CG9472-RA (CG9472), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141215.2 |
CG9804-RA (CG9804), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132481.1 |
CG1738-RA (CG1738), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_167922.1 |
CG1828-RB, transcript variant B (dre4), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_057262.2 |
CG1828-RA, transcript variant A (dre4), mRNA |
| 0 |
0 |
0 |
3 |
10 |
NM_169052.1 |
CG2666-RB, transcript variant B (kkv), mRNA |
| 0 |
0 |
0 |
3 |
10 |
NM_079509.1 |
CG2666-RA, transcript variant A (kkv), mRNA |
| 0 |
0 |
0 |
3 |
10 |
NM_206430.1 |
CG2666-RC, transcript variant C (kkv), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_137027.1 |
CG4744-RA (CG4744), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_137584.1 |
CG15120-RA (CG15120), mRNA |
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