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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15262R-1 |
| Symbol |
CG15262 |
Full Name |
CG15262 |
| CG No |
CG15262 |
Old CG No |
CG15262 |
| Synonyms |
BG:DS07851.5, CG15262 |
| Accession No (Link to NCBI) |
NM_135906.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACAATACTGGGGATGACCAGC |
| Primer Seq. 3' |
aatctagaggtaccTAAACGGAACGGATTGCCGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACAATACTGG GGATGACCAG CAGGAGGTGG AGAGCTCTCC AAGTCCGCCG CCTCCTCCGC
0061 CTTTCCAATC GCCATTATCA TCGCCCGAGT CTTTGCAGAC CCCGGATGGA TATCCCATTT
0121 TGTACGTGGA TGTGGTAAAG AATAAGCCCA AACAGGAGTT GCCGCCGTCC GCATCACCGT
0181 CCCAACCACT TGCGCCGGAG TTCAGCATCT TTCGCGAAGA TTTTCCGGCT CTGCCAGGAT
0241 CATTACCATC TGCATTACCA ACCGCATTAC CAACGACATC GAGCCCTGTG GTGCCCGACG
0301 ACTGGACAGC CATGATGAGC AACAGCGAGC AGGTCGAACT CGCCAAATTT CGAAATACAG
0361 TTATCCGTAA TTCCAGTGAT CCGTGCGTTA ACGCTAACGG CAACGAGGTG CTCCGTCAGC
0421 CAACCCATGC TCCCGAGAAG CGCCAGAATA TGGAGTTGCT GCCCCATATG TACGGTTATC
0481 TCGGCAATCC GTTCCGTTTA |
| in silico PCR Fragment |
0001 ACAATACTGG GGATGACCAG CAGGAGGTGG AGAGCTCTCC AAGTCCGCCG CCTCCTCCGC
0061 CTTTCCAATC GCCATTATCA TCGCCCGAGT CTTTGCAGAC CCCGGATGGA TATCCCATTT
0121 TGTACGTGGA TGTGGTAAAG AATAAGCCCA AACAGGAGTT GCCGCCGTCC GCATCACCGT
0181 CCCAACCACT TGCGCCGGAG TTCAGCATCT TTCGCGAAGA TTTTCCGGCT CTGCCAGGAT
0241 CATTACCATC TGCATTACCA ACCGCATTAC CAACGACATC GAGCCCTGTG GTGCCCGACG
0301 ACTGGACAGC CATGATGAGC AACAGCGAGC AGGTCGAACT CGCCAAATTT CGAAATACAG
0361 TTATCCGTAA TTCCAGTGAT CCGTGCGTTA ACGCTAACGG CAACGAGGTG CTCCGTCAGC
0421 CAACCCATGC TCCCGAGAAG CGCCAGAATA TGGAGTTGCT GCCCCATATG TACGGTTATC
0481 TCGGCAATCC GTTCCGTTTA |
| Assemble Data |
15262R-1.IR_full 1 ACAATACTGGGGATGACCAGCAGGAGGTGGAGAGCTCTCCAAGTCCGCCGCCTCCTCCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 1 ACAATACTGGGGATGACCAGCAGGAGGTGGAGAGCTCTCCAAGTCCGCCGCCTCCTCCGC 60
15262R-1.IR_full 61 CTTTCCAATCGCCATTATCATCGCCCGAGTCTTTGCAGACCCCGGATGGATATCCCATTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 61 CTTTCCAATCGCCATTATCATCGCCCGAGTCTTTGCAGACCCCGGATGGATATCCCATTT 120
15262R-1.IR_full 121 TGTACGTGGATGTGGTAAAGAATAAGCCCAAACAGGAGTTGCCGCCGTCCGCATCACCGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 121 TGTACGTGGATGTGGTAAAGAATAAGCCCAAACAGGAGTTGCCGCCGTCCGCATCACCGT 180
15262R-1.IR_full 181 CCCAACCACTTGCGCCGGAGTTCAGCATCTTTCGCGAAGATTTTCCGGCTCTGCCAGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 181 CCCAACCACTTGCGCCGGAGTTCAGCATCTTTCGCGAAGATTTTCCGGCTCTGCCAGGAT 240
15262R-1.IR_full 241 CATTACCATCTGCATTACCAACCGCATTACCAACGACATCGAGCCCTGTGGTGCCCGACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 241 CATTACCATCTGCATTACCAACCGCATTACCAACGACATCGAGCCCTGTGGTGCCCGACG 300
15262R-1.IR_full 301 ACTGGACAGCCATGATGAGCAACAGCGAGCAGGTCGAACTCGCCAAATTTCGAAATACAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 301 ACTGGACAGCCATGATGAGCAACAGCGAGCAGGTCGAACTCGCCAAATTTCGAAATACAG 360
15262R-1.IR_full 361 TTATCCGTAATTCCAGTGATCCGTGCGTTAACGCTAACGGCAACGAGGTGCTCCGTCAGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 361 TTATCCGTAATTCCAGTGATCCGTGCGTTAACGCTAACGGCAACGAGGTGCTCCGTCAGC 420
15262R-1.IR_full 421 CAACCCATGCTCCCGAGAAGCGCCAGAATATGGAGTTGCTGCCCCATATGTACGGTTATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15262R-1.in silico 421 CAACCCATGCTCCCGAGAAGCGCCAGAATATGGAGTTGCTGCCCCATATGTACGGTTATC 480
15262R-1.IR_full 481 TCGGCAATCCGTTCCGTTTA 500
||||||||||||||||||||
15262R-1.in silico 481 TCGGCAATCCGTTCCGTTTA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
10 |
8 |
NM_135906.2 |
CG15262-RA (CG15262), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_165508.1 |
CG11084-RA, transcript variant A (pk), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_165512.1 |
CG11084-RC, transcript variant C (pk), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_165509.1 |
CG11084-RB, transcript variant B (pk), mRNA |
| 0 |
0 |
3 |
4 |
8 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078887.1 |
CG8667-RA (dimm), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_134551.4 |
CG32506-RA (CG32506), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_164449.1 |
CG4244-RC, transcript variant C (Su(dx)), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_164448.1 |
CG4244-RA, transcript variant A (Su(dx)), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_057405.2 |
CG4244-RB, transcript variant B (Su(dx)), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_136690.2 |
CG1513-RA (CG1513), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132794.2 |
CG18210-RA (CG18210), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_144390.1 |
CG18744-RA (CG18744), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_079113.2 |
CG4012-RA (gek), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_131988.1 |
CG3309-RA (CG3309), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136186.1 |
CG16798-RA (CG16798), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142316.1 |
CG14906-RA (CG14906), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138115.2 |
CG30421-RA (CG30421), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130725.2 |
CG15239-RA (CG15239), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132154.1 |
CG4615-RA (CG4615), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167027.2 |
CG6824-RC, transcript variant C (ovo), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167026.2 |
CG6824-RB, transcript variant B (ovo), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079058.2 |
CG5186-RA, transcript variant A (slim), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166289.1 |
CG5186-RB, transcript variant B (slim), mRNA |
| 0 |
0 |
1 |
2 |
11 |
NM_168575.1 |
CG32138-RA, transcript variant A (CG32138), mRNA |
| 0 |
0 |
1 |
2 |
11 |
NM_168576.1 |
CG32138-RB, transcript variant B (CG32138), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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