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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
15035R-1 |
| Symbol |
CG15035 |
Full Name |
CG15035 |
| CG No |
CG15035 |
Old CG No |
CG15035 |
| Synonyms |
CG15035 |
| Accession No (Link to NCBI) |
NM_132168.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTATCGAAGAAATGCGCCGAAG |
| Primer Seq. 3' |
aatctagaggtaccCACAGGATCGCATTAGGGTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TATCGAAGAA ATGCGCCGAA GGATTCAGCA AGGCATCAAC AGATGCTGTG AACAACTGCT
0061 TGAAATAGCC ATGATTTTAA CCCATAAAGG TGGTCAGGTT TTAAACCGTT TGAAGGGAGC
0121 ATCATTCCCG GAGCTCGCCC AAAATAGTGA AAATATCGGT AATGGTTCAA TGTCCGAAAA
0181 TCAGAATCAA AGGCTCGATC CCATGACAAG TGCACAATAT ACTACTTTAG GATTTGGAAA
0241 CTTTGATGGC GAGGGGGACT ACTTGCGGCA GAAGAATAAA ATCAACATTC AACTTCCGCG
0301 TCTTGTGAGC GTGACTTGCG GCTTTGCCAA GGATCACATA AGATATCCGG AATATAATCG
0361 TGGAAAGTTT GGTGAGGATG CCTGGTTCAT GTCCTCCAGT CCACAGGCGT GCATAATGGG
0421 CGTGGCCGAT GGAGTGGGCG GTTGGCGAAA TTATGGCGTT GATCCCGGAA AATTCAGTAT
0481 GACCCTAATG CGATCCTGTG |
| in silico PCR Fragment |
0001 TATCGAAGAA ATGCGCCGAA GGATTCAGCA AGGCATCAAC AGATGCTGTG AACAACTGCT
0061 TGAAATAGCC ATGATTTTAA CCCATAAAGG TGGTCAGGTT TTAAACCGTT TGAAGGGAGC
0121 ATCATTCCCG GAGCTCGCCC AAAATAGTGA AAATATCGGT AATGGTTCAA TGTCCGAAAA
0181 TCAGAATCAA AGGCTCGATC CCATGACAAG TGCACAATAT ACTACTTTAG GATTTGGAAA
0241 CTTTGATGGC GAGGGGGACT ACTTGCGGCA GAAGAATAAA ATCAACATTC AACTTCCGCG
0301 TCTTGTGAGC GTGACTTGCG GCTTTGCCAA GGATCACATA AGATATCCGG AATATAATCG
0361 TGGAAAGTTT GGTGAGGATG CCTGGTTCAT GTCCTCCAGT CCACAGGCGT GCATAATGGG
0421 CGTGGCCGAT GGAGTGGGCG GTTGGCGAAA TTATGGCGTT GATCCCGGAA AATTCAGTAT
0481 GACCCTAATG CGATCCTGTG |
| Assemble Data |
15035R-1.IR_full 1 TATCGAAGAAATGCGCCGAAGGATTCAGCAAGGCATCAACAGATGCTGTGAACAACTGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 1 TATCGAAGAAATGCGCCGAAGGATTCAGCAAGGCATCAACAGATGCTGTGAACAACTGCT 60
15035R-1.IR_full 61 TGAAATAGCCATGATTTTAACCCATAAAGGTGGTCAGGTTTTAAACCGTTTGAAGGGAGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 61 TGAAATAGCCATGATTTTAACCCATAAAGGTGGTCAGGTTTTAAACCGTTTGAAGGGAGC 120
15035R-1.IR_full 121 ATCATTCCCGGAGCTCGCCCAAAATAGTGAAAATATCGGTAATGGTTCAATGTCCGAAAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 121 ATCATTCCCGGAGCTCGCCCAAAATAGTGAAAATATCGGTAATGGTTCAATGTCCGAAAA 180
15035R-1.IR_full 181 TCAGAATCAAAGGCTCGATCCCATGACAAGTGCACAATATACTACTTTAGGATTTGGAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 181 TCAGAATCAAAGGCTCGATCCCATGACAAGTGCACAATATACTACTTTAGGATTTGGAAA 240
15035R-1.IR_full 241 CTTTGATGGCGAGGGGGACTACTTGCGGCAGAAGAATAAAATCAACATTCAACTTCCGCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 241 CTTTGATGGCGAGGGGGACTACTTGCGGCAGAAGAATAAAATCAACATTCAACTTCCGCG 300
15035R-1.IR_full 301 TCTTGTGAGCGTGACTTGCGGCTTTGCCAAGGATCACATAAGATATCCGGAATATAATCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 301 TCTTGTGAGCGTGACTTGCGGCTTTGCCAAGGATCACATAAGATATCCGGAATATAATCG 360
15035R-1.IR_full 361 TGGAAAGTTTGGTGAGGATGCCTGGTTCATGTCCTCCAGTCCACAGGCGTGCATAATGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 361 TGGAAAGTTTGGTGAGGATGCCTGGTTCATGTCCTCCAGTCCACAGGCGTGCATAATGGG 420
15035R-1.IR_full 421 CGTGGCCGATGGAGTGGGCGGTTGGCGAAATTATGGCGTTGATCCCGGAAAATTCAGTAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15035R-1.in silico 421 CGTGGCCGATGGAGTGGGCGGTTGGCGAAATTATGGCGTTGATCCCGGAAAATTCAGTAT 480
15035R-1.IR_full 481 GACCCTAATGCGATCCTGTG 500
||||||||||||||||||||
15035R-1.in silico 481 GACCCTAATGCGATCCTGTG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_132168.1 |
CG15035-RA (CG15035), mRNA |
| 0 |
0 |
5 |
3 |
4 |
NM_136289.3 |
CG3651-RA (CG3651), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_176119.2 |
CG33135-RB, transcript variant B (KCNQ), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_176120.2 |
CG33135-RA, transcript variant A (KCNQ), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132961.2 |
CG4955-RA (CG4955), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164473.1 |
CG31681-RA (CG31681), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079491.2 |
CG5723-RB (Ten-m), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141993.2 |
CG7518-RB, transcript variant B (CG7518), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169489.1 |
CG7518-RA, transcript variant A (CG7518), mRNA |
| 0 |
0 |
0 |
15 |
6 |
NM_143467.2 |
CG7615-RA (fig), mRNA |
| 0 |
0 |
0 |
10 |
24 |
NM_139362.2 |
CG12091-RA (CG12091), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_139823.1 |
CG14825-RA (BBS1), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_135095.1 |
CG14014-RA (CG14014), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_140007.1 |
CG5747-RA (mfr), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_057816.3 |
CG13263-RA (Cyt-c-d), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_138002.2 |
CG5339-RA (CG5339), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_164458.1 |
CG10874-RB, transcript variant B (CG10874), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_134808.2 |
CG10874-RA, transcript variant A (CG10874), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_176385.1 |
CG33214-RA (CG33214), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141697.1 |
CG9461-RA (CG9461), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_142857.2 |
CG17121-RA (CG17121), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_079436.1 |
CG17121-RA (CG17121), mRNA, small, or homeotic discs 1 CG8887-RA (ash1), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_135124.1 |
CG14001-RA (bchs), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_136623.1 |
CG8014-RA (Rme-8), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_165900.1 |
CG8830-RB, transcript variant B (CG8830), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_136940.1 |
CG8830-RA, transcript variant A (CG8830), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_142354.1 |
CG4090-RA (CG4090), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_144407.2 |
CG18787-RA (CG18787), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_164981.1 |
CG18789-RA (CG18789), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_140458.1 |
CG17177-RA (CG17177), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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