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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 15008R-1 
 Symbol Cpr64Ac  Full Name Cuticular protein 64Ac 
 CG No CG15008  Old CG No CG15008 
 Synonyms Cpr64Ac, CG15008 
 Accession No (Link to NCBI) NM_139617.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGATTGCCCAGACTCTGTTC 
 Primer Seq. 3' 
aatctagaggtaccTTCTTCTCCACGACCGCATTG 
 Predicted Fragment Size 
452 
 IR fragment full Seq 
0001 ATGATTGCCC AGACTCTGTT CGTTTTGGGA CTGATCCTGT CCGCCGTTGT GGCCATTCCC 
0061 ATTGATCCCT ATGGACTTTC GGCCCCTGGA CTCACCTATG CGGCTCCCAA GTTGCTTGCT 
0121 GCTCCTGCCA TTTCCTATGC TGCTCCCAAA CTCCTGGCGG CTCCTGCCAT CTCGTATGCC 
0181 GCTCCTGCCA TCTCCTATGC TCCCAAGGTC CTGGCTGCCC CCGTCGCTGT GGCCAAGGTG 
0241 GCNTGTTNGC CGAGCCCTAC GATCCTAATC CGCAGTACAG CTTCTCGTAC GGAGTAACCG 
0301 ATCATCACAC CGGTGACTCC AAGCAGCAGG AGGAGACCCT GGTCAACGGA GTCGTCCACG 
0361 GCAGCTACTC CCTGGCCGAA CCCGATGGCA CTATTCGCAA GGTCACCTAC ACCGCCGACA 
0421 AGGTCAACGG ATTCAATGCG GTCGTGGAGA AGAA 
 in silico PCR Fragment 
0001 ATGATTGCCC AGACTCTGTT CGTTTTGGGA CTGATCCTGT CCGCCGTTGT GGCCATTCCC 
0061 ATTGATCCCT ATGGACTTTC GGCCCCTGGA CTCACCTATG CGGCTCCCAA GTTGCTTGCT 
0121 GCTCCTGCCA TTTCCTATGC TGCTCCCAAA CTCCTGGCGG CTCCTGCCAT CTCGTATGCC 
0181 GCTCCTGCCA TCTCCTATGC TCCCAAGGTC CTGGCTGCCC CCGTCGCTGT GGCCAAGGTG 
0241 GC-TGTT-GC CGAGCCCTAC GATCCCAATC CGCAGTACAG CTTCTCGTAC GGAGTAACCG 
0301 ATCATCACAC CGGTGACTCC AAGCAGCAGG AGGAGACCCT GGTCAACGGA GTCGTCCACG 
0361 GCAGCTACTC CCTGGCCGAA CCCGATGGCA CTATTCGCAA GGTCACCTAC ACCGCCGACA 
0421 AGGTCAACGG ATTCAATGCG GTCGTGGAGA AGAA 
 Assemble Data 

15008R-1.IR_full       1   ATGATTGCCCAGACTCTGTTCGTTTTGGGACTGATCCTGTCCGCCGTTGTGGCCATTCCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     1   ATGATTGCCCAGACTCTGTTCGTTTTGGGACTGATCCTGTCCGCCGTTGTGGCCATTCCC 60


15008R-1.IR_full       61  ATTGATCCCTATGGACTTTCGGCCCCTGGACTCACCTATGCGGCTCCCAAGTTGCTTGCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     61  ATTGATCCCTATGGACTTTCGGCCCCTGGACTCACCTATGCGGCTCCCAAGTTGCTTGCT 120


15008R-1.IR_full       121 GCTCCTGCCATTTCCTATGCTGCTCCCAAACTCCTGGCGGCTCCTGCCATCTCGTATGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     121 GCTCCTGCCATTTCCTATGCTGCTCCCAAACTCCTGGCGGCTCCTGCCATCTCGTATGCC 180


15008R-1.IR_full       181 GCTCCTGCCATCTCCTATGCTCCCAAGGTCCTGGCTGCCCCCGTCGCTGTGGCCAAGGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     181 GCTCCTGCCATCTCCTATGCTCCCAAGGTCCTGGCTGCCCCCGTCGCTGTGGCCAAGGTG 240


15008R-1.IR_full       241 GCNTGTTNGCCGAGCCCTACGATCCTAATCCGCAGTACAGCTTCTCGTACGGAGTAACCG 300
                           || |||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||
15008R-1.in silico     241 GC-TGTT-GCCGAGCCCTACGATCCCAATCCGCAGTACAGCTTCTCGTACGGAGTAACCG 300


15008R-1.IR_full       301 ATCATCACACCGGTGACTCCAAGCAGCAGGAGGAGACCCTGGTCAACGGAGTCGTCCACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     301 ATCATCACACCGGTGACTCCAAGCAGCAGGAGGAGACCCTGGTCAACGGAGTCGTCCACG 360


15008R-1.IR_full       361 GCAGCTACTCCCTGGCCGAACCCGATGGCACTATTCGCAAGGTCACCTACACCGCCGACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15008R-1.in silico     361 GCAGCTACTCCCTGGCCGAACCCGATGGCACTATTCGCAAGGTCACCTACACCGCCGACA 420


15008R-1.IR_full       421 AGGTCAACGGATTCAATGCGGTCGTGGAGAAGAA 454
                           ||||||||||||||||||||||||||||||||||
15008R-1.in silico     421 AGGTCAACGGATTCAATGCGGTCGTGGAGAAGAA 454



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   434  10  24  53  NM_139617.1  CG15008-RA (CG15008), mRNA 
0.23   1  4  6  25  NM_205956.1  CG33302-RA (CG33302), mRNA 
0   0  4  3  13  NM_132038.1  CG4052-RA (CG4052), mRNA 
0   0  4  3  3  NM_169060.1  CG4052-RA (CG4052), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA 
0   0  4  3  3  NM_169061.1  CG4052-RA (CG4052), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA 
0   0  3  14  44  NM_140866.1  CG9299-RA (CG9299), mRNA 
0   0  2  11  18  NM_139615.2  CG15006-RA (CG15006), mRNA 
0   0  2  6  2  NM_206670.1  CG33224-RA (CG33224), mRNA 
0   0  2  3  10  NM_140613.1  CG13042-RA (CG13042), mRNA 
0   0  2  2  2  NM_168816.1  CG8522-RB, transcript variant B (HLH106), mRNA 
0   0  2  2  2  NM_168815.1  CG8522-RA, transcript variant A (HLH106), mRNA 
0   0  2  2  2  NM_079442.2  CG8522-RC, transcript variant C (HLH106), mRNA 
0   0  1  9  15  NM_140601.2  CG13065-RA (CG13065), mRNA 
0   0  1  5  7  NM_139616.1  CG15007-RA (CG15007), mRNA 
0   0  1  4  20  NM_142556.2  CG6240-RA (CG6240), mRNA 
0   0  1  4  7  NM_164492.1  CG31690-RB, transcript variant B (CG31690), mRNA 
0   0  1  4  7  NM_205893.1  CG31690-RA, transcript variant A (CG31690), mRNA 
0   0  1  4  4  NM_137024.2  CG10814-RA (CG10814), mRNA 
0   0  1  3  6  NM_132990.2  CG8557-RA, transcript variant A (CG8557), mRNA 
0   0  1  3  6  NM_176751.1  CG8557-RB, transcript variant B (CG8557), mRNA 
0   0  1  3  2  NM_135887.2  CG3491-RA (CG3491), mRNA 
0   0  1  3  2  NM_140438.2  CG32139-RA (Sox21b), mRNA 
0   0  1  2  12  NM_140605.1  CG13047-RA (CG13047), mRNA 
0   0  1  2  7  NM_078878.2  CG10123-RA (Top3alpha), mRNA 
0   0  1  2  5  NM_164944.1  CG31869-RA (CG31869), mRNA 
0   0  1  2  3  NM_166296.1  CG30332-RA (CG30332), mRNA 
0   0  1  2  3  NM_132989.2  CG8568-RA (CG8568), mRNA 
0   0  0  27  73  NM_137799.1  CG13504-RA (CG13504), mRNA 
0   0  0  8  13  NM_057558.3  CG16963-RA (Cry), mRNA 
0   0  0  6  16  NM_164879.1  CG31876-RA (CG31876), mRNA 

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