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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1499R-4 
 Symbol CG1499  Full Name CG1499 
 CG No CG1499  Old CG No CG1499 
 Synonyms clone 2.47, anon-EST:Liang-2.47, CG1499 
 Accession No (Link to NCBI) NM_143614.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTACTGCTGCTGATCTGCCTAA 
 Primer Seq. 3' 
aatctagaggtaccGGTCGTCTTGAACAGCACACA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TACTGCTGCT GATCTGCCTA ATTGCGAGTG ACGCCGCCAG CGCCGCACGC AAAACAAAGC 
0061 GTCCCGTCAA GCCGGTCAAG CAGACCAATC CGCGCACCAA TGTAACGCCT TCGCCGCCgG 
0121 NTGTCGCCTC ATCGGGTAGT TCCACACCCG CCAGCACCAG CAGCACCACC ACCACGGGAA 
0181 GGAAGCGGCG ACCAGGAGGT GGCCACAGTC GGATCTGCAG CTCTAGCCGG CGGCGGGGAA 
0241 CTGCCCAAGC CTCTGGTCAC GcCCATCGAT NGCGCAGCCG GAGGCTAAGA CGGACAAGGC 
0301 ACCAGCTCTG GAGGAGGAGT GCGATCCAGA TATGATTGGC TTTGAGATTA TTACCGGCTA 
0361 CGTGCTTTCG GCTCCCTCGA AAATGCTGGA CACTCTGCCC GGCNACGCTG ATGCTAACCG 
0421 ACTGCTTGGA GGCCTGCCAA AATAACGAGT CCTGCAGCGC TGTCAACTAC GAAACCGGAT 
0481 TGTGTGTGCT GTTCAAGACG ACC 
 in silico PCR Fragment 
0001 TACTGCTGCT GATCTGCCTA ATTGCGAGTG ACGCCGCCAG CGCCGCACGC AAAACAAAGC 
0061 GTCCCGTCAA GCCGGTCAAG CAGACCAATC CGCGCACCAA TGTAACGCCT TCGCCGCCGG 
0121 CTGTCGCCTC ATCGGGTAGT TCCACACCCG CCAGCACCAG CAGCACCACC ACCAC-GGAA 
0181 GGAAGCGGCG ACCAGGAGGT GGCCACAGTC GGATCTGCAG CTCTAGCCGG CGGCGGGGAA 
0241 CTGCCCAAGC CTCTGGTCAC GCCCATCGAG -GCGCAGCCG GAGGCTAAGA CGGACAAGGC 
0301 ACCAGCTCTG GAGGAGGAGT GCGATCCAGA TATGATTGGC TTTGAGATTA TTACCGGCTA 
0361 CGTGCTTTCG GCTCCCTCGA AAATGCTGGA CACTCTGCCC GGC-ACGCTG ATGCTAACCG 
0421 ACTGCTTGGA GGCCTGCCAA AGTAACGAGT CCTGCAGCGC TGTCAACTAC GAAACCGGAT 
0481 TGTGTGTGCT GTTCAAGACG ACC 
 Assemble Data 

1499R-4.IR_full       1   TACTGCTGCTGATCTGCCTAATTGCGAGTGACGCCGCCAGCGCCGCACGCAAAACAAAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1499R-4.in silico     1   TACTGCTGCTGATCTGCCTAATTGCGAGTGACGCCGCCAGCGCCGCACGCAAAACAAAGC 60


1499R-4.IR_full       61  GTCCCGTCAAGCCGGTCAAGCAGACCAATCCGCGCACCAATGTAACGCCTTCGCCGCCGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1499R-4.in silico     61  GTCCCGTCAAGCCGGTCAAGCAGACCAATCCGCGCACCAATGTAACGCCTTCGCCGCCGG 120


1499R-4.IR_full        121 TGTCGCCTCATCGGGTAGTTCCACACCCGCCAGCACCAGCAGCACCACCACCACGGGAA 179
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
1499R-4.in silico      121 TGTCGCCTCATCGGGTAGTTCCACACCCGCCAGCACCAGCAGCACCACCACCAC-GGAA 179


1499R-4.IR_full       181 GGAAGCGGCGACCAGGAGGTGGCCACAGTCGGATCTGCAGCTCTAGCCGGCGGCGGGGAA 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1499R-4.in silico     181 GGAAGCGGCGACCAGGAGGTGGCCACAGTCGGATCTGCAGCTCTAGCCGGCGGCGGGGAA 239


1499R-4.IR_full       241 CTGCCCAAGCCTCTGGTCACGCCCATCGATNGCGCAGCCGGAGGCTAAGACGGACAAGGC 299
                          |||||||||||||||||||||||||||||  |||||||||||||||||||||||||||||
1499R-4.in silico     241 CTGCCCAAGCCTCTGGTCACGCCCATCGAG-GCGCAGCCGGAGGCTAAGACGGACAAGGC 299


1499R-4.IR_full       301 ACCAGCTCTGGAGGAGGAGTGCGATCCAGATATGATTGGCTTTGAGATTATTACCGGCTA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1499R-4.in silico     301 ACCAGCTCTGGAGGAGGAGTGCGATCCAGATATGATTGGCTTTGAGATTATTACCGGCTA 359


1499R-4.IR_full       361 CGTGCTTTCGGCTCCCTCGAAAATGCTGGACACTCTGCCCGGCNACGCTGATGCTAACCG 419
                          ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
1499R-4.in silico     361 CGTGCTTTCGGCTCCCTCGAAAATGCTGGACACTCTGCCCGGC-ACGCTGATGCTAACCG 419


1499R-4.IR_full       421 ACTGCTTGGAGGCCTGCCAAAATAACGAGTCCTGCAGCGCTGTCAACTACGAAACCGGAT 479
                          ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
1499R-4.in silico     421 ACTGCTTGGAGGCCTGCCAAAGTAACGAGTCCTGCAGCGCTGTCAACTACGAAACCGGAT 479


1499R-4.IR_full       481 TGTGTGTGCTGTTCAAGACGACC 502
                          |||||||||||||||||||||||
1499R-4.in silico     481 TGTGTGTGCTGTTCAAGACGACC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  11  NM_143614.1  CG1499-RA, transcript variant A (CG1499), mRNA 
100   482  0  0  11  NM_170552.1  CG1499-RB, transcript variant B (CG1499), mRNA 
0.62   3  7  15  38  NM_134519.1  CG11940-RA, transcript variant A (CG11940), mRNA 
0.62   3  7  15  37  NM_167686.1  CG11940-RB, transcript variant B (CG11940), mRNA 
0.62   3  5  8  79  NM_079224.3  CG10037-RA (vvl), mRNA 
0.41   2  5  5  20  NM_139548.2  CG12016-RA, transcript variant A (CG12016), mRNA 
0.41   2  5  5  20  NM_168022.1  CG12016-RB, transcript variant B (CG12016), mRNA 
0.41   2  3  2  14  NM_142297.2  CG14900-RA (Cad89D), mRNA 
0.2   1  11  21  31  NM_206440.1  CG31481-RC, transcript variant C (pb), mRNA 
0.2   1  11  21  31  NM_206439.1  CG31481-RD, transcript variant D (pb), mRNA 
0.2   1  11  21  31  NM_057321.4  CG31481-RA, transcript variant A (pb), mRNA 
0.2   1  11  21  31  NM_206441.1  CG31481-RB, transcript variant B (pb), mRNA 
0.2   1  5  14  81  NM_001014630.1  CG33547-RA (Rim), mRNA 
0.2   1  4  7  15  NM_057438.3  CG6172-RA, transcript variant A (vnd), mRNA 
0.2   1  4  6  13  NM_001042788.1  CG6172-RB, transcript variant B (vnd), mRNA 
0.2   1  3  8  61  NM_132114.1  CG14442-RA (CG14442), mRNA 
0.2   1  3  3  11  NM_205956.1  CG33302-RA (CG33302), mRNA 
0.2   1  2  6  11  NM_136859.2  CG8991-RA (Sobp), mRNA 
0.2   1  2  4  9  NM_136511.2  CG2158-RA (CG2158), mRNA 
0   0  10  19  42  NM_143613.2  CG12063-RA (CG12063), mRNA 
0   0  9  39  72  NM_080120.2  CG14560-RA (msopa), mRNA 
0   0  9  22  46  NM_167647.2  CG12199-RB, transcript variant B (kek5), mRNA 
0   0  9  22  46  NM_133154.2  CG12199-RA, transcript variant A (kek5), mRNA 
0   0  9  9  19  NM_141158.3  CG7369-RA (CG7369), mRNA 
0   0  8  21  35  NM_170366.1  CG14066-RB, transcript variant B (larp), mRNA 
0   0  8  21  35  NM_170365.1  CG14066-RC, transcript variant C (larp), mRNA 
0   0  8  21  35  NM_080259.1  CG14066-RA, transcript variant A (larp), mRNA 
0   0  8  13  14  NM_144180.1  CG14148-RA (CG14148), mRNA 
0   0  7  26  58  NM_136646.1  CG13953-RA (CG13953), mRNA 
0   0  7  26  38  NM_136593.1  CG8181-RA (CG8181), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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