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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14997R-2 
 Symbol CG14997  Full Name CG14997 
 CG No CG14997  Old CG No CG14997 
 Synonyms BcDNA:GH04863, CG14997 
 Accession No (Link to NCBI) NM_139620.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGAACCGTCGCCTTCCAGGA 
 Primer Seq. 3' 
aatctagaggtaccTTACTGTCGGGGGTCTCCAGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGAACCGTC GCCTTCCAGG AACCATCAGC CAGTGCCAGA AGCTTCTCCT AGCCACCCAC 
0061 AACCATGCTC CAACGGCCTT CTCCACCAGA TTCCTGTCCA CATCTCGAGT GAACCGCGAG 
0121 CGGCAAGAAT GCCAAGTGCT GGTTGTGGGC GGTGGCACCG GTGGGTGTGC CATGGCCGCC 
0181 AAGTTGTCCT CACGTTTGGG CAGCGACAAG GTGGTCGTCC TGGAACCGGA AGATAAACAT 
0241 TACTACCAAC CCATGTTCAC CCTAATAGGA GGTGGTATGA AGAGATTGGA TCAGAGCCAC 
0301 CGCCAAATGG CCGATGTGCT GCCCACAAAG GCCAAATGGG TGAAGGAGAA GGCGCTGGAG 
0361 TTCGATCCGG ATAATAATAC GGTTAGCACT TCTGGTGGCA AGACCATCAA GTACGACTTC 
0421 CTGATCATCG CCACCGGACT GCAGCTCAAT TATGGAAAGA TACCCGGCCT GGTGGAGGCC 
0481 CTGGAGACCC CCGACAGTAA  
 in silico PCR Fragment 
0001 ATGAACCGTC GCCTTCCAGG AACCATCAGC CAGTGCCAGA AGCTTCTCCT AGCCACCCAC 
0061 AACCATGCTC CAACGGCCTT CTCCACCAGA TTCCTGTCCA CATCTCGAGT GAACCGCGAG 
0121 CGGCAAGAAT GCCAAGTGCT GGTTGTGGGC GGTGGCACCG GTGGGTGTGC CATGGCCGCC 
0181 AAGTTGTCCT CACGTTTGGG CAGCGACAAG GTGGTCGTCC TGGAACCGGA AGATAAACAT 
0241 TACTACCAAC CCATGTTCAC CCTAATAGGA GGTGGTATGA AGAGATTGGA TCAGAGCCAC 
0301 CGCCAAATGG CCGATGTGCT GCCCACAAAG GCCAAATGGG TGAAGGAGAA GGCGCTGGAG 
0361 TTCGATCCGG ATAATAATAC GGTTAGCACT TCTGGTGGCA AGACCATCAA GTACGACTTC 
0421 CTGATCATCG CCACCGGACT GCAGCTCAAT TATGGAAAGA TACCTGGCCT GGTGGAGGCC 
0481 CTGGAGACCC CCGACAGTAA  
 Assemble Data 

14997R-2.IR_full       1   ATGAACCGTCGCCTTCCAGGAACCATCAGCCAGTGCCAGAAGCTTCTCCTAGCCACCCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     1   ATGAACCGTCGCCTTCCAGGAACCATCAGCCAGTGCCAGAAGCTTCTCCTAGCCACCCAC 60


14997R-2.IR_full       61  AACCATGCTCCAACGGCCTTCTCCACCAGATTCCTGTCCACATCTCGAGTGAACCGCGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     61  AACCATGCTCCAACGGCCTTCTCCACCAGATTCCTGTCCACATCTCGAGTGAACCGCGAG 120


14997R-2.IR_full       121 CGGCAAGAATGCCAAGTGCTGGTTGTGGGCGGTGGCACCGGTGGGTGTGCCATGGCCGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     121 CGGCAAGAATGCCAAGTGCTGGTTGTGGGCGGTGGCACCGGTGGGTGTGCCATGGCCGCC 180


14997R-2.IR_full       181 AAGTTGTCCTCACGTTTGGGCAGCGACAAGGTGGTCGTCCTGGAACCGGAAGATAAACAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     181 AAGTTGTCCTCACGTTTGGGCAGCGACAAGGTGGTCGTCCTGGAACCGGAAGATAAACAT 240


14997R-2.IR_full       241 TACTACCAACCCATGTTCACCCTAATAGGAGGTGGTATGAAGAGATTGGATCAGAGCCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     241 TACTACCAACCCATGTTCACCCTAATAGGAGGTGGTATGAAGAGATTGGATCAGAGCCAC 300


14997R-2.IR_full       301 CGCCAAATGGCCGATGTGCTGCCCACAAAGGCCAAATGGGTGAAGGAGAAGGCGCTGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     301 CGCCAAATGGCCGATGTGCTGCCCACAAAGGCCAAATGGGTGAAGGAGAAGGCGCTGGAG 360


14997R-2.IR_full       361 TTCGATCCGGATAATAATACGGTTAGCACTTCTGGTGGCAAGACCATCAAGTACGACTTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14997R-2.in silico     361 TTCGATCCGGATAATAATACGGTTAGCACTTCTGGTGGCAAGACCATCAAGTACGACTTC 420


14997R-2.IR_full       421 CTGATCATCGCCACCGGACTGCAGCTCAATTATGGAAAGATACCCGGCCTGGTGGAGGCC 480
                           |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
14997R-2.in silico     421 CTGATCATCGCCACCGGACTGCAGCTCAATTATGGAAAGATACCTGGCCTGGTGGAGGCC 480


14997R-2.IR_full       481 CTGGAGACCCCCGACAGTAA 500
                           ||||||||||||||||||||
14997R-2.in silico     481 CTGGAGACCCCCGACAGTAA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168071.1  CG14997-RB, transcript variant B (CG14997), mRNA 
100   482  0  0  0  NM_139620.1  CG14997-RA, transcript variant A (CG14997), mRNA 
0   0  3  4  4  NM_132072.1  CG14446-RA (CG14446), mRNA 
0   0  3  3  5  NM_139549.1  CG11526-RB, transcript variant B (CG11526), mRNA 
0   0  3  3  5  NM_168023.1  CG11526-RA, transcript variant A (CG11526), mRNA 
0   0  2  5  12  NM_134961.1  CG10019-RA (CG10019), mRNA 
0   0  2  2  3  NM_168743.2  CG32177-RA (CG32177), mRNA 
0   0  2  2  3  NM_137119.2  CG10105-RA (CG10105), mRNA 
0   0  2  2  2  NM_168210.1  CG8582-RA, transcript variant A (Sh3beta), mRNA 
0   0  2  2  2  NM_139855.2  CG8582-RB, transcript variant B (Sh3beta), mRNA 
0   0  1  4  3  NM_138027.2  CG11388-RA (CG11388), mRNA 
0   0  1  3  2  NM_001038982.1  CG33970-RA, transcript variant A (CG33970), mRNA 
0   0  1  2  12  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   0  1  2  5  NM_165342.3  CG9326-RB, transcript variant B (CG9326), mRNA 
0   0  1  2  5  NM_165343.2  CG9326-RC, transcript variant C (CG9326), mRNA 
0   0  1  2  5  NM_206011.2  CG9326-RD, transcript variant D (CG9326), mRNA 
0   0  1  2  4  NM_057328.3  CG3127-RA (Pgk), mRNA 
0   0  1  2  4  NM_136193.1  CG2493-RA (CG2493), mRNA 
0   0  1  2  3  NM_132310.2  CG2194-RB, transcript variant B (Reg-3), mRNA 
0   0  1  2  3  NM_167182.1  CG2194-RC, transcript variant C (Reg-3), mRNA 
0   0  1  2  2  NM_168549.1  CG10089-RB, transcript variant B (CG10089), mRNA 
0   0  1  2  2  NM_168548.1  CG10089-RA, transcript variant A (CG10089), mRNA 
0   0  1  2  2  NM_140397.1  CG10089-RD, transcript variant D (CG10089), mRNA 
0   0  0  6  22  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  6  5  NM_142065.1  CG9927-RA (Art6), mRNA 
0   0  0  6  3  NM_140862.1  CG9452-RA (CG9452), mRNA 
0   0  0  5  11  NM_205940.1  CG10595-RB, transcript variant B (d), mRNA 
0   0  0  5  11  NM_205939.1  CG10595-RC, transcript variant C (d), mRNA 
0   0  0  5  11  NM_175991.1  CG10595-RA, transcript variant A (d), mRNA 
0   0  0  5  5  NM_136158.2  CG10538-RA (CdGAPr), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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