NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14990R-2

Symbol CG14990 Full Name CG14990

CG No CG14990 Old CG No CG14990

Synonyms SPH97, CG14990

Accession No (Link to NCBI) NM_139599.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGCTTGGTGGCCAATGTGAA

Primer Seq. 3'

aatctagaggtaccTCAACTGATCCTGGCACTGCG

Predicted Fragment Size

IR fragment full Seq

0001 TGGCTTGGTG GCCAATGTGA AGGTGCCCAA AGATTACTCC ACACCTGGCC AATTTCCATG 
0061 GGTGGTTGCC CTTTTCAGTC AGGGAAAATA CTTTGGGGCC GGTTCTTTGA TTGTCCCCAG 
0121 AAGCTAGTCC TTACAGCGGC TAGCATTGTG GTGGGCAAGA CTGATGCGGA AATTGTGGTG 
0181 AGAGCAGGTC GAATGGAATA CGGGACAACG CAGTGAATTC CTACCGTCGG AGGATCGTCC 
0241 GGTGGCCAGC AGTTGTGCAA CACAGGGAGT TTTCGTATTT ATTGGGAGCC AATAACATTG 
0301 CCCTTCTGTT CCTAGCCAAC CCATTTGAGT TGAAAAGCCA CATCCGAACC ATCTGCTTGC 
0361 CCAGCAAGGG AAGGTCCTTC GATCAGAAGc GCTGCTTGGT GACCGGATGG GGAAAGGTGG 
0421 CATTCAACGA TGAAAATTAC TCAAATATCC AAAAGAAAAT CGAACTGCCG ATGATTAACA 
0481 GGGCGCAGTG CCAGGATCAG TTGA

in silico PCR Fragment

0001 TGGCTTGGTG GCCAATGTGA AGGTGCCCAA AGATTACTCC ACACCTGGCC AATTTCCATG 
0061 GGTGGTTGCC CTTTTCAGTC AGGGAAAATA CTTTGGGGCC GGTTCTTTGA TTG-CCCCAG 
0121 AAG-TAGTCC TTACAGCGGC TAGCATTGTG GTGGGCAAGA CTGATGCGGA AATTGTGGTG 
0181 AGAGCAGG-C GAATGGAATA CGGGACAACG CAGTGAATTC CTACCGTCGG AGGATCGTCC 
0241 GGTGGCCAG- AGTTGTGCAA CACAGGGAGT TTTCGTATTT ATTGGGAGCC AATAACATTG 
0301 CCCTTCTGTT CCTAGCCAAC CCATTTGAGT TGAAAAGCCA CATCCGAACC ATCTGCTTGC 
0361 CCAGCCAGGG AAGGTCCTTC GATCAGAAGC GCTGCTTGGT GACCGGATGG GGAAAGGTGG 
0421 CATTCAACGA TGAAAATTAC TCAAATATCC AAAAGAAAAT CGAACTGCCG ATGATTAACA 
0481 GGGCGCAGTG CCAGGATCAG TTGA

Assemble Data


14990R-2.IR_full       1   TGGCTTGGTGGCCAATGTGAAGGTGCCCAAAGATTACTCCACACCTGGCCAATTTCCATG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     1   TGGCTTGGTGGCCAATGTGAAGGTGCCCAAAGATTACTCCACACCTGGCCAATTTCCATG 60


14990R-2.IR_full       61  GGTGGTTGCCCTTTTCAGTCAGGGAAAATACTTTGGGGCCGGTTCTTTGATTGTCCCCAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
14990R-2.in silico     61  GGTGGTTGCCCTTTTCAGTCAGGGAAAATACTTTGGGGCCGGTTCTTTGATTG-CCCCAG 120


14990R-2.IR_full       121 AAGCTAGTCCTTACAGCGGCTAGCATTGTGGTGGGCAAGACTGATGCGGAAATTGTGGTG 180
                           ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     121 AAG-TAGTCCTTACAGCGGCTAGCATTGTGGTGGGCAAGACTGATGCGGAAATTGTGGTG 180


14990R-2.IR_full       181 AGAGCAGGTCGAATGGAATACGGGACAACGCAGTGAATTCCTACCGTCGGAGGATCGTCC 240
                           |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     181 AGAGCAGG-CGAATGGAATACGGGACAACGCAGTGAATTCCTACCGTCGGAGGATCGTCC 240


14990R-2.IR_full       241 GGTGGCCAGCAGTTGTGCAACACAGGGAGTTTTCGTATTTATTGGGAGCCAATAACATTG 300
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     241 GGTGGCCAG-AGTTGTGCAACACAGGGAGTTTTCGTATTTATTGGGAGCCAATAACATTG 300


14990R-2.IR_full       301 CCCTTCTGTTCCTAGCCAACCCATTTGAGTTGAAAAGCCACATCCGAACCATCTGCTTGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     301 CCCTTCTGTTCCTAGCCAACCCATTTGAGTTGAAAAGCCACATCCGAACCATCTGCTTGC 360


14990R-2.IR_full       361 CCAGCAAGGGAAGGTCCTTCGATCAGAAGCGCTGCTTGGTGACCGGATGGGGAAAGGTGG 420
                           ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     361 CCAGCCAGGGAAGGTCCTTCGATCAGAAGCGCTGCTTGGTGACCGGATGGGGAAAGGTGG 420


14990R-2.IR_full       421 CATTCAACGATGAAAATTACTCAAATATCCAAAAGAAAATCGAACTGCCGATGATTAACA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14990R-2.in silico     421 CATTCAACGATGAAAATTACTCAAATATCCAAAAGAAAATCGAACTGCCGATGATTAACA 480


14990R-2.IR_full       481 GGGCGCAGTGCCAGGATCAGTTGA 504
                           ||||||||||||||||||||||||
14990R-2.in silico     481 GGGCGCAGTGCCAGGATCAGTTGA 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139599.1	CG14990-RA (CG14990), mRNA
0	0	2	3	2	NM_078992.2	CG8581-RA, transcript variant A (fra), mRNA
0	0	2	3	2	NM_165913.1	CG8581-RB, transcript variant B (fra), mRNA
0	0	1	7	4	NM_134680.1	CG13691-RA (BBS8), mRNA
0	0	1	6	12	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	1	6	2	NM_130505.2	CG13359-RA (CG13359), mRNA
0	0	1	3	4	NM_057638.3	CG14472-RA (poe), mRNA
0	0	1	3	2	NM_140321.1	CG10660-RA (CG10660), mRNA
0	0	1	3	2	NM_078759.2	CG11020-RA, transcript variant A (nompC), mRNA
0	0	1	3	2	NM_205912.1	CG11020-RB, transcript variant B (nompC), mRNA
0	0	1	2	2	NM_143427.2	CG1458-RA (CG1458), mRNA
0	0	1	2	2	NM_136534.2	CG2121-RA (CG2121), mRNA
0	0	1	1	2	NM_001043302.1	CG11958-RD, transcript variant D (Cnx99A), mRNA
0	0	1	1	2	NM_001043301.1	CG11958-RC, transcript variant C (Cnx99A), mRNA
0	0	1	1	2	NM_057809.4	CG11958-RB, transcript variant B (Cnx99A), mRNA
0	0	1	1	2	NM_170407.2	CG11958-RA, transcript variant A (Cnx99A), mRNA
0	0	0	12	2	NM_135530.2	CG5390-RA (CG5390), mRNA
0	0	0	6	5	NM_206533.1	CG5911-RB, transcript variant B (ETHR), mRNA
0	0	0	6	5	NM_142703.2	CG5911-RA, transcript variant A (ETHR), mRNA
0	0	0	5	2	NM_136000.2	CG6453-RA (CG6453), mRNA
0	0	0	4	13	NM_134877.1	CG18557-RA (CG18557), mRNA
0	0	0	4	9	NM_134801.1	CG4259-RA (CG4259), mRNA
0	0	0	4	6	NM_079750.3	CG5436-RA (Hsp68), mRNA
0	0	0	4	3	NM_001015108.1	CG41049-PA.3 (CG41049), mRNA
0	0	0	3	7	NM_079700.1	CG3723-RA (Dhc93AB), mRNA
0	0	0	3	4	NM_078653.1	CG18572-RA, transcript variant A (r), mRNA
0	0	0	3	4	NM_138154.2	CG32475-RA (mthl8), mRNA
0	0	0	3	3	NM_058104.4	CG3400-RB, transcript variant B (Pfrx), mRNA
0	0	0	3	3	NM_058103.2	CG3400-RG, transcript variant G (Pfrx), mRNA
0	0	0	3	3	NM_168837.1	CG17233-RC, transcript variant C (CG17233), mRNA

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