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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14782R-1 
 Symbol CG14782  Full Name CG14782 
 CG No CG14782  Old CG No CG14782 
 Synonyms EG:80H7.5, CG14782 
 Accession No (Link to NCBI) NM_130567.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTGTCGCAAGCGGCCCAAG 
 Primer Seq. 3' 
aatctagaggtaccAAGCGCTCGTAGCAGGCGTCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGTGTCGCA AGCGGCCCAA GTCGCGCCAG TTCTTCCTGT TCAACGACAT CCTGGTGTAC 
0061 GGAAACATAG TGATTGGCAA GAAGAAGTAC AACAAGCAGC ATATCATGCC GCTGGAGGAG 
0121 GTCTCGCTGG AATCGATTGC CGACAACCAG ACGTACAGAA ACGGCTGGTA CATCCGCACC 
0181 ACCACCAAGT CGTTTGTGGT GTTTGCGGCC ACCAGCACCG AGAAGCAGGA GTGGATGGCT 
0241 CACATCAACA AGTGCGTGGA GGACCTGCTG CGCAAGAGCG GCAAGAAGCC GGTGGAGAAC 
0301 CACGCCGCCG TTTGGGTGCC CGACACCGAC GCCAGCGTCT GCATGCACTG CAAGAAGACG 
0361 CAGTTTACTT TCATCCAGCG CCGGCATCAC TGCCGCAACT GCGgCGCTGT TGTCTGCGCC 
0421 GGCTGCTCGG CCAAGAAGTT CCTGCTTCCG CAGCAGAGCA CCAAGGCGTT GCGCGTCTGT 
0481 GACGCCTGCT ACGAGCGCTT  
 in silico PCR Fragment 
0001 ATGTGTCGCA AGCGGCCCAA GTCGCGCCAG TTCTTCCTGT TCAACGACAT CCTGGTGTAC 
0061 GGAAACATAG TGATTGGCAA GAAGAAGTAC AACAAGCAGC ATATCATGCC GCTGGAGGAG 
0121 GTCTCGCTGG AATCGATTGC CGACAACCAG ACGTACAGAA ACGGCTGGTA CATCCGCACC 
0181 ACCACCAAGT CGTTTGTGGT GTTTGCGGCC ACCAGCACCG AGAAGCAGGA GTGGATGGCT 
0241 CACATCAACA AGTGCGTGGA GGACCTGCTG CGCAAGAGCG GCAAGAAGCC GGTGGAGAAC 
0301 CACGCCGCCG TTTGGGTGCC CGACACCGAC GCCAGCGTCT GCATGCACTG CAAGAAGACG 
0361 CAGTTTACTT TCATCCAGCG CCGGCATCAC TGCCGCAACT GCGGCGCTGT TGTCTGCGCC 
0421 GGCTGCTCGG CCAAGAAGTT CCTGCTTCCG CAGCAGAGCA CCAAGGCGTT GCGCGTCTGT 
0481 GACGCCTGCT ACGAGCGCTT  
 Assemble Data 

14782R-1.IR_full       1   ATGTGTCGCAAGCGGCCCAAGTCGCGCCAGTTCTTCCTGTTCAACGACATCCTGGTGTAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     1   ATGTGTCGCAAGCGGCCCAAGTCGCGCCAGTTCTTCCTGTTCAACGACATCCTGGTGTAC 60


14782R-1.IR_full       61  GGAAACATAGTGATTGGCAAGAAGAAGTACAACAAGCAGCATATCATGCCGCTGGAGGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     61  GGAAACATAGTGATTGGCAAGAAGAAGTACAACAAGCAGCATATCATGCCGCTGGAGGAG 120


14782R-1.IR_full       121 GTCTCGCTGGAATCGATTGCCGACAACCAGACGTACAGAAACGGCTGGTACATCCGCACC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     121 GTCTCGCTGGAATCGATTGCCGACAACCAGACGTACAGAAACGGCTGGTACATCCGCACC 180


14782R-1.IR_full       181 ACCACCAAGTCGTTTGTGGTGTTTGCGGCCACCAGCACCGAGAAGCAGGAGTGGATGGCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     181 ACCACCAAGTCGTTTGTGGTGTTTGCGGCCACCAGCACCGAGAAGCAGGAGTGGATGGCT 240


14782R-1.IR_full       241 CACATCAACAAGTGCGTGGAGGACCTGCTGCGCAAGAGCGGCAAGAAGCCGGTGGAGAAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     241 CACATCAACAAGTGCGTGGAGGACCTGCTGCGCAAGAGCGGCAAGAAGCCGGTGGAGAAC 300


14782R-1.IR_full       301 CACGCCGCCGTTTGGGTGCCCGACACCGACGCCAGCGTCTGCATGCACTGCAAGAAGACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     301 CACGCCGCCGTTTGGGTGCCCGACACCGACGCCAGCGTCTGCATGCACTGCAAGAAGACG 360


14782R-1.IR_full       361 CAGTTTACTTTCATCCAGCGCCGGCATCACTGCCGCAACTGCGGCGCTGTTGTCTGCGCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     361 CAGTTTACTTTCATCCAGCGCCGGCATCACTGCCGCAACTGCGGCGCTGTTGTCTGCGCC 420


14782R-1.IR_full       421 GGCTGCTCGGCCAAGAAGTTCCTGCTTCCGCAGCAGAGCACCAAGGCGTTGCGCGTCTGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14782R-1.in silico     421 GGCTGCTCGGCCAAGAAGTTCCTGCTTCCGCAGCAGAGCACCAAGGCGTTGCGCGTCTGT 480


14782R-1.IR_full       481 GACGCCTGCTACGAGCGCTT 500
                           ||||||||||||||||||||
14782R-1.in silico     481 GACGCCTGCTACGAGCGCTT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_130567.2  CG14782-RA (CG14782), mRNA 
0   0  5  2  7  NM_080148.2  CG2522-RA (Gtp-bp), mRNA 
0   0  4  2  4  NM_140308.2  CG4300-RB, transcript variant B (CG4300), mRNA 
0   0  4  2  4  NM_168496.1  CG4300-RA, transcript variant A (CG4300), mRNA 
0   0  3  6  2  NM_135450.1  CG17855-RA (CG17855), mRNA 
0   0  3  2  4  NM_079503.2  CG9761-RA (Nep2), mRNA 
0   0  2  7  2  NM_079749.4  CG5405-RA, transcript variant A (KrT95D), mRNA 
0   0  2  7  2  NM_170108.2  CG5405-RB, transcript variant B (KrT95D), mRNA 
0   0  2  7  2  NM_170110.2  CG5405-RC, transcript variant C (KrT95D), mRNA 
0   0  2  5  20  NM_078604.3  CG32592-RA (hiw), mRNA 
0   0  2  5  7  NM_134770.1  CG17657-RA (CG17657), mRNA 
0   0  2  3  9  NM_166315.1  CG12758-RB, transcript variant B (sano), mRNA 
0   0  2  3  9  NM_166314.1  CG12758-RA, transcript variant A (sano), mRNA 
0   0  2  3  9  NM_166316.1  CG12758-RC, transcript variant C (sano), mRNA 
0   0  2  3  9  NM_079064.2  CG12758-RD, transcript variant D (sano), mRNA 
0   0  2  3  6  NM_132268.1  CG12772-RA (CG12772), mRNA 
0   0  2  3  5  NM_168932.1  CG32446-RA (CG32446), mRNA 
0   0  2  3  5  NM_141069.2  CG7611-RA, transcript variant A (CG7611), mRNA 
0   0  2  3  3  NM_168915.1  CG7611-RD, transcript variant D (CG7611), mRNA 
0   0  2  3  3  NM_168914.1  CG7611-RC, transcript variant C (CG7611), mRNA 
0   0  2  3  3  NM_168919.1  CG7611-RH, transcript variant H (CG7611), mRNA 
0   0  2  3  3  NM_168913.1  CG7611-RB, transcript variant B (CG7611), mRNA 
0   0  2  3  3  NM_168918.1  CG7611-RG, transcript variant G (CG7611), mRNA 
0   0  2  3  3  NM_168917.1  CG7611-RF, transcript variant F (CG7611), mRNA 
0   0  2  3  3  NM_168916.1  CG7611-RE, transcript variant E (CG7611), mRNA 
0   0  1  6  2  NM_141322.1  CG1236-RA (CG1236), mRNA 
0   0  1  5  2  NM_170324.1  CG31064-RB, transcript variant B (CG31064), mRNA 
0   0  1  4  9  NM_166992.2  CG2904-RA (ec), mRNA 
0   0  1  4  8  NM_167922.1  CG1828-RB, transcript variant B (dre4), mRNA 
0   0  1  4  8  NM_057262.2  CG1828-RA, transcript variant A (dre4), mRNA 

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