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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
14763R-1 |
| Symbol |
CG14763 |
Full Name |
CG14763 |
| CG No |
CG14763 |
Old CG No |
CG14763 |
| Synonyms |
CG14763 |
| Accession No (Link to NCBI) |
NM_136496.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAAACTGGCAAGGGCAAAAA |
| Primer Seq. 3' |
aatctagaggtaccCGTAGTACAGGTAAAAGGTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAAACTGGC AAGGGCAAAA AGGAGAAGAA AGAAAAGTCG GCCGAGGGCA CTAAGCCGAG
0061 GCCGGCAAGC AGCCAGCGCG GTATCGCTGA CCAAGTCCCA TATGGGCGTT TCCTTGCCGA
0121 TGGGTGCTCC GGCAGCCAAG CCCACCATGC GGTTCATGCC CACCTACCGC CTGGAGTCCA
0181 AGAATCCGCT CAACAAGGAA CGCGTGGAGA ACATCATAAA GGCGGTGATG AACCGACACT
0241 ACAACGATGA GTACATGTTC CATCCCAAGC ATTCCCTCCA CATGGCCGCG CAAGTGAGCG
0301 AGGAAATCAA GAACCGCATC AAACTCGACA ACTACGACAG ATATCGCTAC ATAGTGCTGG
0361 TCACTGTGGG CGAGTTCCTG ATGCAGGGCC TCTACTCCAT GGTCAACTTC CTGTGGGATG
0421 CTGAAAAAGA CGGATTTGTG ACCTACAGCG TGGAGAGACC CTCGTACTTT GCAGTCTGCA
0481 CCACCTTTTA CCTGTACTAC G |
| in silico PCR Fragment |
0001 GGAAACTGGC AAGGGCAAAA AGGAGAAGAA AGAAAAGTCG GCCGAGGGCA CTAAGCCGAG
0061 GCCGGCAAGC AGCCAGCGCG G-ATCGCTGA CCAAGTCCCA TATGGGCGTT TCCTTGCCGA
0121 TGGGTGCTCC GGCAGCCAAG CCCACCATGC GGTTCATGCC CACCTACCGC CTGGAGTCCA
0181 AGAATCCGCT CAACAAGGAA CGCGTGGAGA ACATCATAAA GGCGGTGATG AACCGACACT
0241 ACAACGATGA GTACATGTTC CATCCCAAGC ATTCCCTCCA CATGGCCGCG CAAGTGAGCG
0301 AGGAAATCAA GAACCGCATC AAACTCGACA ACTACGACAG ATATCGCTAC ATAGTGCTGG
0361 TCACTGTGGG CGAGTTCCTG ATGCAGGGCC TCTACTCCAT GGTCAACTTC CTGTGGGATG
0421 CTGAAAAAGA CGGATTTGTG ACCTACAGCG TGGAGAGACC CTCGTACTTT GCAGTCTGCA
0481 CCACCTTTTA CCTGTACTAC G |
| Assemble Data |
14763R-1.IR_full 1 GGAAACTGGCAAGGGCAAAAAGGAGAAGAAAGAAAAGTCGGCCGAGGGCACTAAGCCGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 1 GGAAACTGGCAAGGGCAAAAAGGAGAAGAAAGAAAAGTCGGCCGAGGGCACTAAGCCGAG 60
14763R-1.IR_full 61 GCCGGCAAGCAGCCAGCGCGGTATCGCTGACCAAGTCCCATATGGGCGTTTCCTTGCCGA 120
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 61 GCCGGCAAGCAGCCAGCGCGG-ATCGCTGACCAAGTCCCATATGGGCGTTTCCTTGCCGA 120
14763R-1.IR_full 121 TGGGTGCTCCGGCAGCCAAGCCCACCATGCGGTTCATGCCCACCTACCGCCTGGAGTCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 121 TGGGTGCTCCGGCAGCCAAGCCCACCATGCGGTTCATGCCCACCTACCGCCTGGAGTCCA 180
14763R-1.IR_full 181 AGAATCCGCTCAACAAGGAACGCGTGGAGAACATCATAAAGGCGGTGATGAACCGACACT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 181 AGAATCCGCTCAACAAGGAACGCGTGGAGAACATCATAAAGGCGGTGATGAACCGACACT 240
14763R-1.IR_full 241 ACAACGATGAGTACATGTTCCATCCCAAGCATTCCCTCCACATGGCCGCGCAAGTGAGCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 241 ACAACGATGAGTACATGTTCCATCCCAAGCATTCCCTCCACATGGCCGCGCAAGTGAGCG 300
14763R-1.IR_full 301 AGGAAATCAAGAACCGCATCAAACTCGACAACTACGACAGATATCGCTACATAGTGCTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 301 AGGAAATCAAGAACCGCATCAAACTCGACAACTACGACAGATATCGCTACATAGTGCTGG 360
14763R-1.IR_full 361 TCACTGTGGGCGAGTTCCTGATGCAGGGCCTCTACTCCATGGTCAACTTCCTGTGGGATG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 361 TCACTGTGGGCGAGTTCCTGATGCAGGGCCTCTACTCCATGGTCAACTTCCTGTGGGATG 420
14763R-1.IR_full 421 CTGAAAAAGACGGATTTGTGACCTACAGCGTGGAGAGACCCTCGTACTTTGCAGTCTGCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14763R-1.in silico 421 CTGAAAAAGACGGATTTGTGACCTACAGCGTGGAGAGACCCTCGTACTTTGCAGTCTGCA 480
14763R-1.IR_full 481 CCACCTTTTACCTGTACTACG 501
|||||||||||||||||||||
14763R-1.in silico 481 CCACCTTTTACCTGTACTACG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_136496.2 |
CG14763-RA (CG14763), mRNA |
| 0.62 |
3 |
3 |
2 |
3 |
NM_134830.1 |
CG3597-RA (CG3597), mRNA |
| 0 |
0 |
6 |
3 |
4 |
NM_136669.2 |
CG10459-RA (CG10459), mRNA |
| 0 |
0 |
3 |
7 |
2 |
NM_079769.2 |
CG11783-RA (Hr96), mRNA |
| 0 |
0 |
3 |
3 |
7 |
NM_080014.2 |
CG3064-RB (futsch), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_137538.1 |
CG15100-RA (CG15100), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_079273.2 |
CG4183-RA (Hsp26), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_206222.1 |
CG7036-RB, transcript variant B (rno), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_138163.1 |
CG7036-RA, transcript variant A (rno), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_167401.1 |
CG1517-RB, transcript variant B (na), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_167402.1 |
CG1517-RC, transcript variant C (na), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_137690.2 |
CG10543-RA, transcript variant A (CG10543), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_164765.2 |
CG7075-RB, transcript variant B (CG7075), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_134512.4 |
CG12701-RA, transcript variant A (CG12701), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167685.1 |
CG12701-RB, transcript variant B (CG12701), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_143361.3 |
CG1866-RB, transcript variant B (Moca-cyp), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_170367.1 |
CG1866-RA, transcript variant A (Moca-cyp), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079575.1 |
CG9470-RA (MtnA), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141818.2 |
CG6744-RA (CG6744), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_138136.2 |
CG30427-RB, transcript variant B (CG30427), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_080305.2 |
CG10755-RA (Cyp4ae1), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001032406.1 |
CG33956-RB, transcript variant B (kay), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_133037.2 |
CG7536-RA, transcript variant A (CG7536), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_167599.1 |
CG7536-RB, transcript variant B (CG7536), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_139930.1 |
CG7375-RA (CG7375), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_134988.1 |
CG15432-RA (CG15432), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137150.2 |
CG10209-RA (CG10209), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_140370.1 |
CG11274-RA (SRm160), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134700.2 |
CG3561-RA (CG3561), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137439.1 |
CG5767-RA (CG5767), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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