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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14749R-1 
 Symbol CG14749  Full Name CG14749 
 CG No CG14749  Old CG No CG14749 
 Synonyms CG14749 
 Accession No (Link to NCBI) NM_136556.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93 [ PubMed ID = 26015542 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACATGATGCGGGCGATGGACT 
 Primer Seq. 3' 
aatctagaggtaccTGCCAATTGCTCGCGCTGCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACATGATGCG GGCGATGGAC TGTCTAAATA TGGCAGCGAC GTTGCGCAAC GCGGCTCTGG 
0061 CGTCCGCAAC GTGCACGGGC AGGACTTTGG GCGAGGATCG GGAACCTATA TGGGTGGAGG 
0121 GCAGTCGGAA GACACCTGAG CCACCATTAC CCGAAGAAAG CCCAGCTCCA GAGCCTAATA 
0181 ACGAGATTCC CCTGCTGCCC AAAATAGATT TTGAGCCTAA TATATCCTGC TTTCCCGACC 
0241 TACAGGCTAT CAACGCTAGC ATTGTTAGGC GGGAACTGGA GGAGGAGGGT AAGAGGTGTG 
0301 TCCGCCAGCA GCTAAAGGCG ATAAGGGACA AACAGGACGC CATGCGCCTG TCGCGAGAGA 
0361 CGCAGCAAAG GAAGGAGGAA CGCCAGCGCG ACCAGTTGCA ACAAAAGGCG CTGCGGGAAC 
0421 GCAATGAAAG TCTCCTTATC CAGAAAGCTG ACCAGATGAC TGCGGCACAA CTAGAGGCCC 
0481 AGCAGCGCGA GCAATTGGCA  
 in silico PCR Fragment 
0001 ACATGATGCG GGCGATGGAC TGTCTAAATA TGGCAGCGAC GTTGCGCAAC GCGGCTCTGG 
0061 CGTCCGCAAC GTGCACGGGC AGGACTTTGG GCGAGGATCG GGAACCTATA TGGGTGGAGG 
0121 GCAGTCGGAA GACACCTGAG CCACCATTAC CCGAAGAAAG CCCAGCTCCA GAGCCCAATA 
0181 ACGAGATTCC CCTGCTGCCC AAAATAGATT TTGAGCCTAA TATATCTTGC TTTCCCGACC 
0241 TACAGGCTAT CAACGCTAGC ATTGTTAGGC GGGAACTGGA GGAGGAGGGT AAGAGGTGTG 
0301 TCCGCCAGCA GCTAAAGGCG ATAAGGGACA AACAGGACGC CATGCGCCTG TCGCGAGAGA 
0361 CGCAGCAAAG GAAGGAGGAA CGCCAGCGCG ACCAGTTGCA ACAAAAGGCG CTGCGGGAAC 
0421 GCAATGAAAG TCTCCTTATC CAGAAAGCTG ACCAGATGAC TGCGGCACAA CTAGAGGCCC 
0481 AGCAGCGCGA GCAATTGGCA  
 Assemble Data 

14749R-1.IR_full       1   ACATGATGCGGGCGATGGACTGTCTAAATATGGCAGCGACGTTGCGCAACGCGGCTCTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     1   ACATGATGCGGGCGATGGACTGTCTAAATATGGCAGCGACGTTGCGCAACGCGGCTCTGG 60


14749R-1.IR_full       61  CGTCCGCAACGTGCACGGGCAGGACTTTGGGCGAGGATCGGGAACCTATATGGGTGGAGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     61  CGTCCGCAACGTGCACGGGCAGGACTTTGGGCGAGGATCGGGAACCTATATGGGTGGAGG 120


14749R-1.IR_full       121 GCAGTCGGAAGACACCTGAGCCACCATTACCCGAAGAAAGCCCAGCTCCAGAGCCTAATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
14749R-1.in silico     121 GCAGTCGGAAGACACCTGAGCCACCATTACCCGAAGAAAGCCCAGCTCCAGAGCCCAATA 180


14749R-1.IR_full       181 ACGAGATTCCCCTGCTGCCCAAAATAGATTTTGAGCCTAATATATCCTGCTTTCCCGACC 240
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
14749R-1.in silico     181 ACGAGATTCCCCTGCTGCCCAAAATAGATTTTGAGCCTAATATATCTTGCTTTCCCGACC 240


14749R-1.IR_full       241 TACAGGCTATCAACGCTAGCATTGTTAGGCGGGAACTGGAGGAGGAGGGTAAGAGGTGTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     241 TACAGGCTATCAACGCTAGCATTGTTAGGCGGGAACTGGAGGAGGAGGGTAAGAGGTGTG 300


14749R-1.IR_full       301 TCCGCCAGCAGCTAAAGGCGATAAGGGACAAACAGGACGCCATGCGCCTGTCGCGAGAGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     301 TCCGCCAGCAGCTAAAGGCGATAAGGGACAAACAGGACGCCATGCGCCTGTCGCGAGAGA 360


14749R-1.IR_full       361 CGCAGCAAAGGAAGGAGGAACGCCAGCGCGACCAGTTGCAACAAAAGGCGCTGCGGGAAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     361 CGCAGCAAAGGAAGGAGGAACGCCAGCGCGACCAGTTGCAACAAAAGGCGCTGCGGGAAC 420


14749R-1.IR_full       421 GCAATGAAAGTCTCCTTATCCAGAAAGCTGACCAGATGACTGCGGCACAACTAGAGGCCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14749R-1.in silico     421 GCAATGAAAGTCTCCTTATCCAGAAAGCTGACCAGATGACTGCGGCACAACTAGAGGCCC 480


14749R-1.IR_full       481 AGCAGCGCGAGCAATTGGCA 500
                           ||||||||||||||||||||
14749R-1.in silico     481 AGCAGCGCGAGCAATTGGCA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_136556.2  CG14749-RA (CG14749), mRNA 
0   0  3  3  2  NM_137209.2  CG8214-RA (CG8214), mRNA 
0   0  2  3  2  NM_141818.2  CG6744-RA (CG6744), mRNA 
0   0  2  3  2  NM_168254.1  CG7915-RB, transcript variant B (Ect4), mRNA 
0   0  2  3  2  NM_001043129.1  CG7915-RC, transcript variant C (Ect4), mRNA 
0   0  2  2  4  NM_167950.1  CG1146-RB, transcript variant B (CG1146), mRNA 
0   0  2  2  4  NM_167951.1  CG1146-RC, transcript variant C (CG1146), mRNA 
0   0  2  2  4  NM_139468.1  CG1146-RA, transcript variant A (CG1146), mRNA 
0   0  2  2  2  NM_165897.1  CG30050-RA (CG30050), mRNA 
0   0  1  4  2  NM_135023.1  CG11927-RA (CG11927), mRNA 
0   0  1  3  5  NM_137512.2  CG5469-RD, transcript variant D (Gint3), mRNA 
0   0  1  3  5  NM_166301.1  CG5469-RB, transcript variant B (Gint3), mRNA 
0   0  1  3  5  NM_166302.1  CG5469-RC, transcript variant C (Gint3), mRNA 
0   0  1  2  3  NM_143153.2  CG4719-RA (tankyrase), mRNA 
0   0  1  2  2  NM_164366.1  CG3164-RA, transcript variant A (CG3164), mRNA 
0   0  1  2  2  NM_205881.2  CG3164-RC, transcript variant C (CG3164), mRNA 
0   0  1  2  2  NM_001038776.1  CG3164-RD, transcript variant D (CG3164), mRNA 
0   0  1  2  2  NM_134650.2  CG3164-RB, transcript variant B (CG3164), mRNA 
0   0  1  2  2  NM_134722.1  CG14339-RA (CG14339), mRNA 
0   0  0  6  12  NM_078974.2  CG7776-RA, transcript variant A (E(Pc)), mRNA 
0   0  0  6  12  NM_165836.1  CG7776-RB, transcript variant B (E(Pc)), mRNA 
0   0  0  5  6  NM_166268.2  CG5119-RF, transcript variant F (pAbp), mRNA 
0   0  0  5  6  NM_057319.3  CG5119-RA, transcript variant A (pAbp), mRNA 
0   0  0  5  6  NM_206160.1  CG5119-RH, transcript variant H (pAbp), mRNA 
0   0  0  5  6  NM_166267.1  CG5119-RE, transcript variant E (pAbp), mRNA 
0   0  0  5  6  NM_166265.1  CG5119-RC, transcript variant C (pAbp), mRNA 
0   0  0  5  6  NM_166264.1  CG5119-RB, transcript variant B (pAbp), mRNA 
0   0  0  5  6  NM_166266.1  CG5119-RD, transcript variant D (pAbp), mRNA 
0   0  0  5  6  NM_166269.1  CG5119-RG, transcript variant G (pAbp), mRNA 
0   0  0  4  8  NM_001032253.1  CG7969-RB, transcript variant B (Menl-2), mRNA 

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