NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14745R-2

Symbol PGRP-SC2 Full Name PGRP-SC2

CG No CG14745 Old CG No CG14745

Synonyms PGRP-SC, CG14745, PGRP-SC2

Accession No (Link to NCBI) NM_136566.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Chen H, Zheng X, Zheng Y.
Age-associated loss of lamin-B leads to systemic inflammation and gut hyperplasia.
Cell (2014) 159(4) 829-43 [ PubMed ID = 25417159 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTTCTGGCCGTACTCTTCTGC

Primer Seq. 3'

aatctagaggtaccTTCCAGATGTTGGTGCCGGGG

Predicted Fragment Size

IR fragment full Seq

0001 NTTCTGGCCG TACTCTTCTG CGCCCAAGCC GTTCTCGGCG TGACCATCAT CTCCAAGTCG 
0061 GAGTGGGGCG GCCGTTCCNC CACGAGCAAG ACCTNCGCTG GCCAACTACC TGAGCTACGC 
0121 CGTGATCCAC CACACCGCTG GAAACTACTG CAGCACCAAG GCCGCCTGCA TCACACAGCT 
0181 GCAGAACATC CAGGCCTACC ACATGGACTC CCTGGGCTGG GCCGATATCG GCTACAACTT 
0241 CCTGATCGGC GGAGACGGCA ACGTGTACGA GGGTCGCGGC TGGAACGTTA TGGGTGCTCA 
0301 CGCCACTAAC TGGAACTCCA AGTCTATCGG CATCTCCTTC CTGGGCAACT ACAATACCAA 
0361 CACCCTCACC TCTGCTCAGA TCACCGCTGC CAAGGGTCTG CTCTCCGATG CGGTCAGTCG 
0421 CGGCCAGATC GTTTCCGGAT ACATCCTGTA NGGACATCGG CAGGTCGGCT CCACCGAgtN 
0481 CCCCGGCACC AACATCTGGA A

in silico PCR Fragment

0001 CTTCTGGCCG TACTCTTCTG CGCCCAAGCC GTTCTCGGCG TGACCATCAT CTCCAAGTCG 
0061 GAGTGGGGCG GCCGTTCCGC CACGAGCAAG ACCT-CGCTG GCCAACTACC TGAGCTACGC 
0121 CGTGATCCAC CACACCGCTG GAAACTACTG CAGCACCAAG GCCGCCTGCA TCACACAGCT 
0181 GCAGAACATC CAGGCCTACC ACATGGACTC CCTGGGCTGG GCCGATATCG GCTACAACTT 
0241 CCTGATCGGC GGAGACGGCA ACGTGTACGA GGGTCGCGGC TGGAACGTTA TGGGTGCTCA 
0301 CGCCACTAAC TGGAACTCCA AGTCTATCGG CATCTCCTTC CTGGGCAACT ACAATACCAA 
0361 CACCCTCACC TCTGCTCAGA TCACCGCTGC CAAGGGTCTG CTCTCCGATG CGGTCAGTCG 
0421 CGGCCAGATC GTTTCCGGAT ACATCCTGTA CGGACATCGG CAGGTCGGCT CCACCGAGTG 
0481 CCCCGGCACC AACATCTGGA A

Assemble Data


14745R-2.IR_full        1   TTCTGGCCGTACTCTTCTGCGCCCAAGCCGTTCTCGGCGTGACCATCATCTCCAAGTCG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico      1   TTCTGGCCGTACTCTTCTGCGCCCAAGCCGTTCTCGGCGTGACCATCATCTCCAAGTCG 59


14745R-2.IR_full       61  GAGTGGGGCGGCCGTTCCNCCACGAGCAAGACCTNCGCTGGCCAACTACCTGAGCTACGC 119
                           |||||||||||||||||| ||||||||||||||| |||||||||||||||||||||||||
14745R-2.in silico     61  GAGTGGGGCGGCCGTTCCGCCACGAGCAAGACCT-CGCTGGCCAACTACCTGAGCTACGC 119


14745R-2.IR_full       121 CGTGATCCACCACACCGCTGGAAACTACTGCAGCACCAAGGCCGCCTGCATCACACAGCT 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico     121 CGTGATCCACCACACCGCTGGAAACTACTGCAGCACCAAGGCCGCCTGCATCACACAGCT 179


14745R-2.IR_full       181 GCAGAACATCCAGGCCTACCACATGGACTCCCTGGGCTGGGCCGATATCGGCTACAACTT 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico     181 GCAGAACATCCAGGCCTACCACATGGACTCCCTGGGCTGGGCCGATATCGGCTACAACTT 239


14745R-2.IR_full       241 CCTGATCGGCGGAGACGGCAACGTGTACGAGGGTCGCGGCTGGAACGTTATGGGTGCTCA 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico     241 CCTGATCGGCGGAGACGGCAACGTGTACGAGGGTCGCGGCTGGAACGTTATGGGTGCTCA 299


14745R-2.IR_full       301 CGCCACTAACTGGAACTCCAAGTCTATCGGCATCTCCTTCCTGGGCAACTACAATACCAA 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico     301 CGCCACTAACTGGAACTCCAAGTCTATCGGCATCTCCTTCCTGGGCAACTACAATACCAA 359


14745R-2.IR_full       361 CACCCTCACCTCTGCTCAGATCACCGCTGCCAAGGGTCTGCTCTCCGATGCGGTCAGTCG 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14745R-2.in silico     361 CACCCTCACCTCTGCTCAGATCACCGCTGCCAAGGGTCTGCTCTCCGATGCGGTCAGTCG 419


14745R-2.IR_full       421 CGGCCAGATCGTTTCCGGATACATCCTGTANGGACATCGGCAGGTCGGCTCCACCGAGTN 479
                           |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| 
14745R-2.in silico     421 CGGCCAGATCGTTTCCGGATACATCCTGTACGGACATCGGCAGGTCGGCTCCACCGAGTG 479


14745R-2.IR_full       481 CCCCGGCACCAACATCTGGAA 500
                           |||||||||||||||||||||
14745R-2.in silico     481 CCCCGGCACCAACATCTGGAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136566.1	CG14745-RA (PGRP-SC2), mRNA
7.46	36	29	84	39	NM_136563.1	CG14746-RA (PGRP-SC1a), mRNA
7.46	36	23	84	45	NM_136565.1	CG8577-RA (PGRP-SC1b), mRNA
0	0	3	2	14	NM_132499.2	CG11709-RA (PGRP-SA), mRNA
0	0	2	7	8	NM_140660.1	CG9681-RA (PGRP-SB1), mRNA
0	0	2	3	4	NM_141550.2	CG9716-RA (Cyp313b1), mRNA
0	0	2	2	4	NM_140072.1	CG12525-RA (CG12525), mRNA
0	0	2	2	3	NM_138037.1	CG10339-RA (CG10339), mRNA
0	0	1	6	5	NM_057852.2	CG9976-RA (Lectin-galC1), mRNA
0	0	1	4	4	NM_132915.1	CG15865-RA (CG15865), mRNA
0	0	1	3	5	NM_057405.2	CG4244-RB, transcript variant B (Su(dx)), mRNA
0	0	1	3	5	NM_164448.1	CG4244-RA, transcript variant A (Su(dx)), mRNA
0	0	1	3	5	NM_164449.1	CG4244-RC, transcript variant C (Su(dx)), mRNA
0	0	1	3	3	NM_143704.1	CG2916-RB, transcript variant B (Sep5), mRNA
0	0	1	3	3	NM_165578.1	CG2916-RA, transcript variant A (Sep5), mRNA
0	0	1	3	3	NM_135029.2	CG3036-RA (CG3036), mRNA
0	0	1	3	2	NM_164674.1	CG31643-RA (CG31643), mRNA
0	0	1	2	9	NM_141101.1	CG14566-RA (CG14566), mRNA
0	0	1	2	4	NM_079111.2	CG2827-RA (Tal), mRNA
0	0	1	2	3	NM_136409.1	CG17002-RB (CG17002), mRNA
0	0	1	2	3	NM_167455.1	CG12708-RA (CG12708), mRNA
0	0	1	2	3	NM_143815.2	CG3953-RA (l(3)IX-14), mRNA
0	0	1	2	2	NM_136712.2	CG30011-RC, transcript variant C (gem), mRNA
0	0	1	2	2	NM_165750.2	CG30011-RA, transcript variant A (gem), mRNA
0	0	0	9	9	NM_140659.1	CG9697-RA (PGRP-SB2), mRNA
0	0	0	6	6	NM_141046.3	CG7605-RA (Rab26), mRNA
0	0	0	5	15	NM_132850.2	CG8995-RA (PGRP-LE), mRNA
0	0	0	5	6	NM_140041.2	CG4432-RB, transcript variant B (PGRP-LC), mRNA
0	0	0	5	5	NM_137510.2	CG30122-RB (CG30122), mRNA
0	0	0	5	4	NM_137192.1	CG12958-RA (CG12958), mRNA

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