NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14671R-3

Symbol CG14671 Full Name CG14671

CG No CG14671 Old CG No CG14671

Synonyms CG14671

Accession No (Link to NCBI) NM_141293.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGGCCAGCTACTGGATGACT

Primer Seq. 3'

aatctagaggtaccGCTAAGCAGGGCACTCCTTCG

Predicted Fragment Size

IR fragment full Seq

0001 CGGGCCAGCT ACTGGATGAC TTGTACCTGG ACATGTTCCA CCTCGTTGAG GAGCACACCC 
0061 AGTGTCGGAT AAATTTGGAG CGATGTAACG CCAGTGGNGG CGATNTCTCC TGGCCCGCAC 
0121 TAGATTTCAG CACGGAGGCA GCCAGTGCGT NGTCCACGGC CCAGATTCCC ACGGAGAACA 
0181 GTGCCGAGTT TAACGCCCTC TGCCGGGTCG TGGACTCCAC GGNATGGCGT CTGCATCGAG 
0241 CGACAGGCGG TTGACAAGTC CAAGGGCTTC GTGGAGCCCC TGCATTGGTT CTCGGTACTG 
0301 CCACCGATGA GCTTGCGCNA ATGCTGTTAA CAAGTTCAAG GACTGCATAG AGCTGGTCGC 
0361 CGAGAGCACT AATCTGCAGC GGCAGCTAGG AGAAGCGCTG GATTCGATCA CAAAGCTGCG 
0421 AAGGAGTGCC CTGCTTAGC

in silico PCR Fragment

0001 CGGGCCAGCT ACTGGATGAC TTGTACCTGG ACATGTTCCA CCTCGTTGAG GAGCACACCC 
0061 AGTGTCGGAT AAATTTGGAG CGATGTAACG CCAGTGG-GG CGAT-TCTCC TGGCCCGCAC 
0121 TAGATTTCAG CACGGAGGCA GCCAGTGCGT -GTCCACGGC CCAGATTCCC ACGGAGAACA 
0181 GTGCCGAGTT TAACGCCCTC TGCCGGGTCG TGGACTCCAC GG-ATGGCGT CTGCATCGAG 
0241 CGACAGGCGG TTGACAAGTC CAAGGGCTTC GTGGAGCCCC TGCATTGGTT CTCGGTACTG 
0301 CCACCGATGA GCTTGCGC-A ATGCTGTTAA CAAGTTCAAG GACTGCATAG AGCTGGTCGC 
0361 CGAGAGCACT AATCTGCAGC GGCAGCTAGG AGAAGCGCTG GATTCGATCA CAAAGCTGCG 
0421 AAGGAGTGCC CTGCTTAGC

Assemble Data


14671R-3.IR_full       1   CGGGCCAGCTACTGGATGACTTGTACCTGGACATGTTCCACCTCGTTGAGGAGCACACCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14671R-3.in silico     1   CGGGCCAGCTACTGGATGACTTGTACCTGGACATGTTCCACCTCGTTGAGGAGCACACCC 60


14671R-3.IR_full       61  AGTGTCGGATAAATTTGGAGCGATGTAACGCCAGTGGNGGCGATNTCTCCTGGCCCGCAC 120
                           ||||||||||||||||||||||||||||||||||||| |||||| |||||||||||||||
14671R-3.in silico     61  AGTGTCGGATAAATTTGGAGCGATGTAACGCCAGTGG-GGCGAT-TCTCCTGGCCCGCAC 120


14671R-3.IR_full       121 TAGATTTCAGCACGGAGGCAGCCAGTGCGTNGTCCACGGCCCAGATTCCCACGGAGAACA 180
                           |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
14671R-3.in silico     121 TAGATTTCAGCACGGAGGCAGCCAGTGCGT-GTCCACGGCCCAGATTCCCACGGAGAACA 180


14671R-3.IR_full       181 GTGCCGAGTTTAACGCCCTCTGCCGGGTCGTGGACTCCACGGNATGGCGTCTGCATCGAG 240
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
14671R-3.in silico     181 GTGCCGAGTTTAACGCCCTCTGCCGGGTCGTGGACTCCACGG-ATGGCGTCTGCATCGAG 240


14671R-3.IR_full       241 CGACAGGCGGTTGACAAGTCCAAGGGCTTCGTGGAGCCCCTGCATTGGTTCTCGGTACTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14671R-3.in silico     241 CGACAGGCGGTTGACAAGTCCAAGGGCTTCGTGGAGCCCCTGCATTGGTTCTCGGTACTG 300


14671R-3.IR_full       301 CCACCGATGAGCTTGCGCNAATGCTGTTAACAAGTTCAAGGACTGCATAGAGCTGGTCGC 360
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
14671R-3.in silico     301 CCACCGATGAGCTTGCGC-AATGCTGTTAACAAGTTCAAGGACTGCATAGAGCTGGTCGC 360


14671R-3.IR_full       361 CGAGAGCACTAATCTGCAGCGGCAGCTAGGAGAAGCGCTGGATTCGATCACAAAGCTGCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14671R-3.in silico     361 CGAGAGCACTAATCTGCAGCGGCAGCTAGGAGAAGCGCTGGATTCGATCACAAAGCTGCG 420


14671R-3.IR_full       421 AAGGAGTGCCCTGCTTAGC 439
                           |||||||||||||||||||
14671R-3.in silico     421 AAGGAGTGCCCTGCTTAGC 439

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	416	0	0	4	NM_141293.1	CG14671-RA (CG14671), mRNA
0	0	3	3	2	NM_136439.2	CG11127-RA (CG11127), mRNA
0	0	2	2	4	NM_165880.1	CG8841-RC, transcript variant C (CG8841), mRNA
0	0	2	2	4	NM_165879.1	CG8841-RB, transcript variant B (CG8841), mRNA
0	0	2	2	4	NM_136916.2	CG8841-RA, transcript variant A (CG8841), mRNA
0	0	1	5	5	NM_143127.2	CG10560-RA (CG10560), mRNA
0	0	1	4	5	NM_079356.2	CG18492-RA (Tak1), mRNA
0	0	1	4	5	NM_136086.4	CG31793-RA (CG31793), mRNA
0	0	1	3	7	NM_142581.1	CG4562-RA (CG4562), mRNA
0	0	1	3	2	NM_141145.1	CG14455-RA (CG14455), mRNA
0	0	1	2	4	NM_137617.3	CG11209-RA (ppk6), mRNA
0	0	1	2	4	NM_142188.2	CG6276-RA (CG6276), mRNA
0	0	1	2	3	NM_135371.3	CG7627-RA (CG7627), mRNA
0	0	0	6	4	NM_137962.2	CG3941-RA, transcript variant A (pita), mRNA
0	0	0	5	4	NM_132050.1	CG3011-RA (CG3011), mRNA
0	0	0	5	3	NM_144414.1	CG18811-RA (CG18811), mRNA
0	0	0	4	5	NM_079203.2	CG11348-RA, transcript variant A (nAcRbeta-64B), mRNA
0	0	0	4	5	NM_141729.1	CG12817-RA, transcript variant A (CG12817), mRNA
0	0	0	4	5	NM_001043236.1	CG12817-RB, transcript variant B (CG12817), mRNA
0	0	0	4	3	NM_135309.1	CG7134-RA (CG7134), mRNA
0	0	0	4	3	NM_079261.4	CG4999-RA, transcript variant A (Tsp66E), mRNA
0	0	0	4	3	NM_168299.1	CG4999-RC, transcript variant C (Tsp66E), mRNA
0	0	0	4	3	NM_168298.1	CG4999-RB, transcript variant B (Tsp66E), mRNA
0	0	0	4	3	NM_080325.3	CG6450-RC (lva), mRNA
0	0	0	4	2	NM_141319.1	CG14674-RA (CG14674), mRNA
0	0	0	3	6	NM_001031947.1	CG33926-RA (CG33926), mRNA
0	0	0	3	5	NM_206469.1	CG11870-RD, transcript variant D (CG11870), mRNA
0	0	0	3	5	NM_169337.2	CG11870-RB, transcript variant B (CG11870), mRNA
0	0	0	3	4	NM_140250.1	CG5964-RA (CG5964), mRNA
0	0	0	3	2	NM_137809.1	CG3292-RA (CG3292), mRNA

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