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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14670R-2 
 Symbol CG14670  Full Name CG14670 
 CG No CG14670  Old CG No CG14670 
 Synonyms NEST:bs27h05, CG14670 
 Accession No (Link to NCBI) NM_141285.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGCAGTCCTGGCGCATTCAAA 
 Primer Seq. 3' 
aatctagaggtaccCACCGCCAGGTGGGAATCTAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGCAGTCCTG GCGCATTCAA AAGGCGTGCT CCAAGATCGC CGAGCACCTC GCGCAGCCCT 
0061 CGAGCATCGC CTTCTATACC CTCCAATCGG GCAGCGATGA CGGATTCGAT CCCGCCCTGG 
0121 CCAGCTCGGA GTTGTGCTGC AATCGGAACG CGGCCAAGGT CACGGACATC CTGTGGCTGC 
0181 ACGCCAACCA GCGGGGTTGC TGCCTGCGTC CGCTGCAAAC GCTACACATC ACGCCTTGGA 
0241 TCAGCTTTCC GCCGGCGCCC AGTCTGCTTC CCTTCTCCTA TGCCGCCGAC ACGTTAACGC 
0301 CGGCGTCCAC GCCCACGGAG GCGGACGTCC CGCGCCAACA ACGGGTTTCG CTCTCTGCCC 
0361 AAGGGGAGGA GCGGATGCAG CTGCTCCTTG AGGCCGACAT AGAGCCACTA CAGCGGCCCA 
0421 GCTCCGAGGA CACATCAGCA GTACGGCTGG AAGATTACGG TAAGCTAATT GCTTGGAAAA 
0481 TAGATTCCCA CCTGGCGGTG  
 in silico PCR Fragment 
0001 TGCAGTCCTG GCGCATTCAA AAGGCGTGCT CCAAGATCGC CGAGCACCTC GCGCAGCCCT 
0061 CGAGCATCGC CTTCTATACC CTCCAATCGG GCAGCGATGA CGGATTCGAT CCCGCCCTGG 
0121 CCAGCTCGGA GTTGTGCTGC AATCGGAACG CGGCCAAGGT CACGGACATC CTGTGGCTGC 
0181 ACGCCAACCA GCGGGGTTGC TGCCTGCGTC CGCTGCAAAC GCTACACATC ACGCCTTGGA 
0241 TCAGCTTTCC GCCGGCGCCC AGTCTGCTTC CCTTCTCCTA TGCCGCCGAC ACGTTAACGC 
0301 CGGCGTCCAC GCCCACGGAG GCGGACGTCC CGCGCCAACA ACGGGTTTCG CTCTCTGCCC 
0361 AAGGGGAGGA GCGGATGCAG CTGCTCCTTG AGGCCGACAT AGAGCCACTA CAGCGGCCCA 
0421 GCTCCGAGGA CACATCAGCA GTACGGCTGG AAGATTACGG TAAGCTAATT GCTTGGAAAA 
0481 TAGATTCCCA CCTGGCGGTG  
 Assemble Data 

14670R-2.IR_full       1   TGCAGTCCTGGCGCATTCAAAAGGCGTGCTCCAAGATCGCCGAGCACCTCGCGCAGCCCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     1   TGCAGTCCTGGCGCATTCAAAAGGCGTGCTCCAAGATCGCCGAGCACCTCGCGCAGCCCT 60


14670R-2.IR_full       61  CGAGCATCGCCTTCTATACCCTCCAATCGGGCAGCGATGACGGATTCGATCCCGCCCTGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     61  CGAGCATCGCCTTCTATACCCTCCAATCGGGCAGCGATGACGGATTCGATCCCGCCCTGG 120


14670R-2.IR_full       121 CCAGCTCGGAGTTGTGCTGCAATCGGAACGCGGCCAAGGTCACGGACATCCTGTGGCTGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     121 CCAGCTCGGAGTTGTGCTGCAATCGGAACGCGGCCAAGGTCACGGACATCCTGTGGCTGC 180


14670R-2.IR_full       181 ACGCCAACCAGCGGGGTTGCTGCCTGCGTCCGCTGCAAACGCTACACATCACGCCTTGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     181 ACGCCAACCAGCGGGGTTGCTGCCTGCGTCCGCTGCAAACGCTACACATCACGCCTTGGA 240


14670R-2.IR_full       241 TCAGCTTTCCGCCGGCGCCCAGTCTGCTTCCCTTCTCCTATGCCGCCGACACGTTAACGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     241 TCAGCTTTCCGCCGGCGCCCAGTCTGCTTCCCTTCTCCTATGCCGCCGACACGTTAACGC 300


14670R-2.IR_full       301 CGGCGTCCACGCCCACGGAGGCGGACGTCCCGCGCCAACAACGGGTTTCGCTCTCTGCCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     301 CGGCGTCCACGCCCACGGAGGCGGACGTCCCGCGCCAACAACGGGTTTCGCTCTCTGCCC 360


14670R-2.IR_full       361 AAGGGGAGGAGCGGATGCAGCTGCTCCTTGAGGCCGACATAGAGCCACTACAGCGGCCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     361 AAGGGGAGGAGCGGATGCAGCTGCTCCTTGAGGCCGACATAGAGCCACTACAGCGGCCCA 420


14670R-2.IR_full       421 GCTCCGAGGACACATCAGCAGTACGGCTGGAAGATTACGGTAAGCTAATTGCTTGGAAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14670R-2.in silico     421 GCTCCGAGGACACATCAGCAGTACGGCTGGAAGATTACGGTAAGCTAATTGCTTGGAAAA 480


14670R-2.IR_full       481 TAGATTCCCACCTGGCGGTG 500
                           ||||||||||||||||||||
14670R-2.in silico     481 TAGATTCCCACCTGGCGGTG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141285.1  CG14670-RA (CG14670), mRNA 
0   0  3  2  3  NM_001014673.1  CG6354-RG, transcript variant G (Rb97D), mRNA 
0   0  3  2  3  NM_079796.3  CG6354-RB, transcript variant B (Rb97D), mRNA 
0   0  3  2  3  NM_001014674.1  CG6354-RF, transcript variant F (Rb97D), mRNA 
0   0  3  2  3  NM_170293.2  CG6354-RA, transcript variant A (Rb97D), mRNA 
0   0  3  2  3  NM_001014677.1  CG6354-RC, transcript variant C (Rb97D), mRNA 
0   0  3  2  3  NM_001014672.1  CG6354-RH, transcript variant H (Rb97D), mRNA 
0   0  3  2  3  NM_001014676.1  CG6354-RD, transcript variant D (Rb97D), mRNA 
0   0  3  2  3  NM_001014675.1  CG6354-RE, transcript variant E (Rb97D), mRNA 
0   0  3  2  3  NM_001014671.1  CG6354-RI, transcript variant I (Rb97D), mRNA 
0   0  2  3  3  NM_206053.1  CG18654-RD, transcript variant D (Dgk), mRNA 
0   0  2  3  3  NM_078930.2  CG18654-RA, transcript variant A (Dgk), mRNA 
0   0  2  3  3  NM_165568.1  CG18654-RB, transcript variant B (Dgk), mRNA 
0   0  2  2  4  NM_134871.1  CG3131-RA (Duox), mRNA 
0   0  1  4  6  NM_143997.2  CG14545-RA (CG14545), mRNA 
0   0  1  4  6  NM_001014547.1  CG3333-RB, transcript variant B (Nop60B), mRNA 
0   0  1  4  6  NM_080381.2  CG3333-RA, transcript variant A (Nop60B), mRNA 
0   0  1  3  2  NM_134538.1  CG1504-RA (CG1504), mRNA 
0   0  1  3  2  NM_078734.3  CG9880-RA (Or23a), mRNA 
0   0  1  2  7  NM_001043282.1  CG4467-RB, transcript variant B (CG4467), mRNA 
0   0  1  2  6  NM_142876.3  CG4467-RA, transcript variant A (CG4467), mRNA 
0   0  1  2  2  NM_141988.1  CG14383-RA (CG14383), mRNA 
0   0  0  5  7  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  0  5  5  NM_141771.1  CG14694-RA (CG14694), mRNA 
0   0  0  5  3  NM_132725.2  CG1461-RA (CG1461), mRNA 
0   0  0  4  6  NM_175962.1  CG3294-RB, transcript variant B (CG3294), mRNA 
0   0  0  4  6  NM_135013.2  CG3294-RA, transcript variant A (CG3294), mRNA 
0   0  0  4  5  NM_169143.1  CG10272-RD, transcript variant D (gpp), mRNA 
0   0  0  4  5  NM_141398.1  CG10272-RA, transcript variant A (gpp), mRNA 
0   0  0  4  5  NM_169142.1  CG10272-RB, transcript variant B (gpp), mRNA 

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