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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14625R-2 
 Symbol CG14625  Full Name CG14625 
 CG No CG14625  Old CG No CG14625 
 Synonyms EG:BACR42I17.8, CG14625 
 Accession No (Link to NCBI) NM_130536.1 
 Inserted Chr. lll 
 Insertional Mutation  2 lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGAGAAGGCCTTTCCCGATCA 
 Primer Seq. 3' 
aatctagaggtaccAGGAGGACGACGATCCAGACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGAGAAGGCC TTTCCCGATC AACTGGCTAT GGCAGCTAGC CGTAGGGGCT CCGACGATCG 
0061 TGGTTACTCC AGGCGGGGAT CGGTTACACA AAGCAGATTA AAGGAGGCGC CCAGCAAAGA 
0121 TTACAGCTTT GGCCAGGTGG AATTAAATCT CGTTCGCCCA GAGCACGAAT CGCATCCAAA 
0181 GCAGACCGCA GAATCCGGAT CCGGAGCGTA TGTCAACCTA GAAGACTCCA AATCGGCAGC 
0241 CGAGTATGTG GCCAATGCCA TCAAGTTGGC CTACAAACAG CCACCGACTC AACTGCGAGG 
0301 TCAAAGCCAA GTTCAGAACC CTAGCAACAA CCACATCCAG GCGCAAAAGG AACAGCAACC 
0361 TACCACCCAG AGAAGTTACC AGGAACAGAG AAGGGATAGG GAGATCCTGG AGATGCAATT 
0421 GCAGAAGGAA CTGCATCAGC aGGCCCTGCT CCTGTCCTCA GGACCCTCGT CGCCATCCTC 
0481 GTCTGGATCG TCGTCCTCCT  
 in silico PCR Fragment 
0001 GGAGAAGGCC TTTCCCGATC AACTGGCTAT GGCAGCTAGC CGTAGGGGCT CCGACGATCG 
0061 TGGTTACTCC AGGCGGGGAT CGGTTACACA AAGCAGATTA AAGGAGGCGC CCAGCAAAGA 
0121 TTACAGCTTT GGCCAGGTGG AATTAAATCT CGTTCGCCCA GAGCACGAAT CGCATCCAAA 
0181 GCAGACCGCA GAATCCGGAT CCGGAGCGTA TGTCAACCTA GAAGACTCTA AATCGGCAGC 
0241 CGAGTATGTG GCCAATGCCA TCAAGTTGGC CTACAAACAG CCACCGACTC AACTGCGAGG 
0301 TCAAAGCCAA GTTCAGAACC CTAGCAACAA CCACATCCAG GCGCAAAAGG AACAGCAACC 
0361 TACCACCCAG AGAAGTTACC AGGAACAGAG AAGGGATAGG GAGATCCTGG AGATGCAATT 
0421 GCAGAAGGAA CTGCATCAGC AGGCCCTGCT CCTGTCCTCA GGACCCTCGT CGCCATCCTC 
0481 GTCTGGATCG TCGTCCTCCT  
 Assemble Data 

14625R-2.IR_full       1   GGAGAAGGCCTTTCCCGATCAACTGGCTATGGCAGCTAGCCGTAGGGGCTCCGACGATCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     1   GGAGAAGGCCTTTCCCGATCAACTGGCTATGGCAGCTAGCCGTAGGGGCTCCGACGATCG 60


14625R-2.IR_full       61  TGGTTACTCCAGGCGGGGATCGGTTACACAAAGCAGATTAAAGGAGGCGCCCAGCAAAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     61  TGGTTACTCCAGGCGGGGATCGGTTACACAAAGCAGATTAAAGGAGGCGCCCAGCAAAGA 120


14625R-2.IR_full       121 TTACAGCTTTGGCCAGGTGGAATTAAATCTCGTTCGCCCAGAGCACGAATCGCATCCAAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     121 TTACAGCTTTGGCCAGGTGGAATTAAATCTCGTTCGCCCAGAGCACGAATCGCATCCAAA 180


14625R-2.IR_full       181 GCAGACCGCAGAATCCGGATCCGGAGCGTATGTCAACCTAGAAGACTCCAAATCGGCAGC 240
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
14625R-2.in silico     181 GCAGACCGCAGAATCCGGATCCGGAGCGTATGTCAACCTAGAAGACTCTAAATCGGCAGC 240


14625R-2.IR_full       241 CGAGTATGTGGCCAATGCCATCAAGTTGGCCTACAAACAGCCACCGACTCAACTGCGAGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     241 CGAGTATGTGGCCAATGCCATCAAGTTGGCCTACAAACAGCCACCGACTCAACTGCGAGG 300


14625R-2.IR_full       301 TCAAAGCCAAGTTCAGAACCCTAGCAACAACCACATCCAGGCGCAAAAGGAACAGCAACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     301 TCAAAGCCAAGTTCAGAACCCTAGCAACAACCACATCCAGGCGCAAAAGGAACAGCAACC 360


14625R-2.IR_full       361 TACCACCCAGAGAAGTTACCAGGAACAGAGAAGGGATAGGGAGATCCTGGAGATGCAATT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     361 TACCACCCAGAGAAGTTACCAGGAACAGAGAAGGGATAGGGAGATCCTGGAGATGCAATT 420


14625R-2.IR_full       421 GCAGAAGGAACTGCATCAGCAGGCCCTGCTCCTGTCCTCAGGACCCTCGTCGCCATCCTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14625R-2.in silico     421 GCAGAAGGAACTGCATCAGCAGGCCCTGCTCCTGTCCTCAGGACCCTCGTCGCCATCCTC 480


14625R-2.IR_full       481 GTCTGGATCGTCGTCCTCCT 500
                           ||||||||||||||||||||
14625R-2.in silico     481 GTCTGGATCGTCGTCCTCCT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_130536.1  CG14625-RB (CG14625), mRNA 
0   0  4  2  2  NM_165541.1  CG30498-RA (boca), mRNA 
0   0  3  2  5  NM_135604.2  CG12299-RA (CG12299), mRNA 
0   0  2  3  4  NM_168816.1  CG8522-RB, transcript variant B (HLH106), mRNA 
0   0  2  3  4  NM_168815.1  CG8522-RA, transcript variant A (HLH106), mRNA 
0   0  2  3  4  NM_079442.2  CG8522-RC, transcript variant C (HLH106), mRNA 
0   0  2  2  10  NM_141002.1  CG11451-RA (CG11451), mRNA 
0   0  2  2  8  NM_142524.2  CG6040-RA (CG6040), mRNA 
0   0  2  2  5  NM_079761.2  CG13626-RA (Syx18), mRNA 
0   0  2  2  3  NM_139626.1  CG1273-RB (CG1273), mRNA 
0   0  2  2  2  NM_132317.1  CG12121-RA (CG12121), mRNA 
0   0  1  9  6  NM_132984.1  CG8661-RA (CG8661), mRNA 
0   0  1  6  19  NM_169696.1  CG3992-RA, transcript variant A (srp), mRNA 
0   0  1  6  19  NM_169694.1  CG3992-RB, transcript variant B (srp), mRNA 
0   0  1  4  4  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  1  4  4  NM_001043112.1  CG33484-RB, transcript variant B (zormin), mRNA 
0   0  1  4  4  NM_001043113.1  CG33484-RC, transcript variant C (zormin), mRNA 
0   0  1  4  4  NM_206240.1  CG33484-RA, transcript variant A (zormin), mRNA 
0   0  1  4  3  NM_130652.2  CG8310-RA (CG8310), mRNA 
0   0  1  2  8  NM_166057.1  CG8787-RA, transcript variant A (Asx), mRNA 
0   0  1  2  8  NM_001014527.1  CG8787-RB, transcript variant B (Asx), mRNA 
0   0  1  2  6  NM_132413.1  CG15295-RA (CG15295), mRNA 
0   0  1  2  6  NM_132908.2  CG4453-RA (Nup153), mRNA 
0   0  1  2  3  NM_138249.2  CG9139-RA (CG9139), mRNA 
0   0  1  2  3  NM_135895.2  CG15269-RA (CG15269), mRNA 
0   0  1  2  2  NM_136191.2  CG31688-RA (CG31688), mRNA 
0   0  1  2  2  NM_080245.3  CG17437-RA (wds), mRNA 
0   0  1  2  2  NM_001043270.1  CG5621-RB, transcript variant B (CG5621), mRNA 
0   0  1  2  2  NM_142670.2  CG5621-RA, transcript variant A (CG5621), mRNA 
0   0  1  1  4  NM_165196.1  CG31739-RA (CG31739), mRNA 

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