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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14609R-2 
 Symbol CG14609  Full Name CG14609 
 CG No CG14609  Old CG No CG14609 
 Synonyms CG14609 
 Accession No (Link to NCBI) NM_141447.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATGGACCCCCAATTCCCTAA 
 Primer Seq. 3' 
aatctagaggtaccGTTGTTGATCGTCTTGATCGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATGGACCCC CAATTCCCTA ACCGAGCAGT TCAAGGGAAT GACCGACTTG TTGACGCGTC 
0061 GTTTGGTGTT CAAGAACCAT GAGTCGAAGG CGAATCGCCA GCGGNTATTT TCGAGAGAAC 
0121 AAGCGCCTGA AGTTGCAANT GCCGCGATGG TCGCGCAAAA TTACAGAATA TTCTGATCAA 
0181 TGACAATACC CATAAGATCC GCAACTTTCT CATCAACCAT AANGCCGCTG CAGAGACTGT 
0241 ACCAGAAGAT GCCAATTCAC TTGGTCGTGG ACAACATCAA TCAGCGAACT TTTGTGATGA 
0301 GAAAGGAGCG GGATCGTTTG GAGTTTCGCT TGGATCAGTT GAAGCAGCAT TACAAGGAAC 
0361 AGCTGCTGCG TAGAGCACTT TTGCAGAATC GCATTAAGTA CCAGAACGAA TTTATTCTCG 
0421 ACGAGGAGTT GAAGTCACGA GTTTTTCTTA AGAAGATTGA AAATTCGAAT GTGCNCCTAA 
0481 AGGCGATCAA GACGATCAAC AAC 
 in silico PCR Fragment 
0001 GATGGACCCC CAATTCCCTA ACCGAGCAGT TCAAGGGAAT GACCGACTTG TTGACGCGTC 
0061 GTTTGGTGTT CAAGAACCAT GAGTCGAAGG CGAATCGCCA GCGG-TATTT TCGAGAGAAC 
0121 AAGCGCCTGA AGTTGCAA-T GCCGCGATGG TCGCGCAAAA TTACAGAATA TTCTGATCAA 
0181 TGACAATACC CATAAGATCC GCAACTTTCT CATCAACCAT AA-GCCGCTG CAGAGACTGT 
0241 ACCAGAAGAT GCCAATTCAC TTGGTCGTGG ACAACATCAA TCAGCGAACT TTTGTGATGA 
0301 GAAAGGAGCG GGATCGTTTG GAGTTTCGCT TGGATCAGTT GAAGCAGCAT TACAAGGAAC 
0361 AGCTGCTGCG TAGAGCACTT TTGCAGAATC GCATTAAGTA CCAGAACGAA TTTATTCTCG 
0421 ACGAGGAGTT GAAGTCACGA GTTTTTCTTA AGAAGATTGA AAATTCGAAT GTGCGCCTAA 
0481 AGGCGATCAA GACGATCAAC AAC 
 Assemble Data 

14609R-2.IR_full        1   ATGGACCCCCAATTCCCTAACCGAGCAGTTCAAGGGAATGACCGACTTGTTGACGCGTC 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14609R-2.in silico      1   ATGGACCCCCAATTCCCTAACCGAGCAGTTCAAGGGAATGACCGACTTGTTGACGCGTC 59


14609R-2.IR_full       61  GTTTGGTGTTCAAGAACCATGAGTCGAAGGCGAATCGCCAGCGGNTATTTTCGAGAGAAC 119
                           |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
14609R-2.in silico     61  GTTTGGTGTTCAAGAACCATGAGTCGAAGGCGAATCGCCAGCGG-TATTTTCGAGAGAAC 119


14609R-2.IR_full       121 AAGCGCCTGAAGTTGCAANTGCCGCGATGGTCGCGCAAAATTACAGAATATTCTGATCAA 179
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
14609R-2.in silico     121 AAGCGCCTGAAGTTGCAA-TGCCGCGATGGTCGCGCAAAATTACAGAATATTCTGATCAA 179


14609R-2.IR_full       181 TGACAATACCCATAAGATCCGCAACTTTCTCATCAACCATAANGCCGCTGCAGAGACTGT 239
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
14609R-2.in silico     181 TGACAATACCCATAAGATCCGCAACTTTCTCATCAACCATAA-GCCGCTGCAGAGACTGT 239


14609R-2.IR_full       241 ACCAGAAGATGCCAATTCACTTGGTCGTGGACAACATCAATCAGCGAACTTTTGTGATGA 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14609R-2.in silico     241 ACCAGAAGATGCCAATTCACTTGGTCGTGGACAACATCAATCAGCGAACTTTTGTGATGA 299


14609R-2.IR_full       301 GAAAGGAGCGGGATCGTTTGGAGTTTCGCTTGGATCAGTTGAAGCAGCATTACAAGGAAC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14609R-2.in silico     301 GAAAGGAGCGGGATCGTTTGGAGTTTCGCTTGGATCAGTTGAAGCAGCATTACAAGGAAC 359


14609R-2.IR_full       361 AGCTGCTGCGTAGAGCACTTTTGCAGAATCGCATTAAGTACCAGAACGAATTTATTCTCG 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14609R-2.in silico     361 AGCTGCTGCGTAGAGCACTTTTGCAGAATCGCATTAAGTACCAGAACGAATTTATTCTCG 419


14609R-2.IR_full       421 ACGAGGAGTTGAAGTCACGAGTTTTTCTTAAGAAGATTGAAAATTCGAATGTGCNCCTAA 479
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
14609R-2.in silico     421 ACGAGGAGTTGAAGTCACGAGTTTTTCTTAAGAAGATTGAAAATTCGAATGTGCGCCTAA 479


14609R-2.IR_full       481 AGGCGATCAAGACGATCAACAAC 502
                           |||||||||||||||||||||||
14609R-2.in silico     481 AGGCGATCAAGACGATCAACAAC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141447.2  CG14609-RA (CG14609), mRNA 
0   0  2  2  1  NM_169735.1  CG11648-RC, transcript variant C (Abd-B), mRNA 
0   0  1  3  5  NM_176244.1  CG9696-RE, transcript variant E (dom), mRNA 
0   0  1  3  4  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
0   0  1  3  4  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
0   0  1  3  4  NM_135325.2  CG7227-RA (CG7227), mRNA 
0   0  1  2  5  NM_141569.3  CG8202-RA (CG8202), mRNA 
0   0  1  2  4  NM_168003.2  CG32270-RA, transcript variant A (CG32270), mRNA 
0   0  1  2  4  NM_137566.2  CG9834-RA, transcript variant A (endoB), mRNA 
0   0  1  2  4  NM_139531.3  CG32269-RA, transcript variant A (CG32269), mRNA 
0   0  1  2  4  NM_166340.1  CG9834-RB, transcript variant B (endoB), mRNA 
0   0  1  2  3  NM_142327.2  CG8913-RA (Irc), mRNA 
0   0  1  2  2  NM_166412.1  CG8994-RC, transcript variant C (exu), mRNA 
0   0  1  2  2  NM_166410.1  CG8994-RA, transcript variant A (exu), mRNA 
0   0  1  2  2  NM_166411.1  CG8994-RB, transcript variant B (exu), mRNA 
0   0  1  2  2  NM_130732.1  CG6428-RA (CG6428), mRNA 
0   0  1  2  2  NM_165019.2  CG5792-RB, transcript variant B (CG5792), mRNA 
0   0  0  5  6  NM_170037.1  CG31151-RA, transcript variant A (wge), mRNA 
0   0  0  5  6  NM_170038.1  CG31151-RB, transcript variant B (wge), mRNA 
0   0  0  5  6  NM_001043281.1  CG31151-RC, transcript variant C (wge), mRNA 
0   0  0  5  2  NM_138985.2  CG8947-RA (26-29-p), mRNA 
0   0  0  4  3  NM_136103.2  CG10563-RA (CG10563), mRNA 
0   0  0  4  3  NM_176148.1  CG33145-RB, transcript variant B (CG33145), mRNA 
0   0  0  4  3  NM_176149.1  CG33145-RA, transcript variant A (CG33145), mRNA 
0   0  0  3  8  NM_001038958.1  CG33959-RA, transcript variant A (Octbeta3R), mRNA 
0   0  0  3  8  NM_001038955.1  CG33959-RC, transcript variant C (Octbeta3R), mRNA 
0   0  0  3  8  NM_001038956.1  CG33959-RE, transcript variant E (Octbeta3R), mRNA 
0   0  0  3  8  NM_001038957.1  CG33959-RB, transcript variant B (Octbeta3R), mRNA 
0   0  0  3  8  NM_001038954.1  CG33959-RD, transcript variant D (Octbeta3R), mRNA 
0   0  0  3  7  NM_170523.2  CG1322-RA, transcript variant A (zfh1), mRNA 

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