NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14532R-3

Symbol CG14532 Full Name CG14532

CG No CG14532 Old CG No CG14532

Synonyms CG14532

Accession No (Link to NCBI) NM_135321.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees male pupal lethal, female semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAAGCTGACAGCAGCCAAA

Primer Seq. 3'

aatctagaggtaccGCAAATATTGACGACGACCGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGAAGCTGA CAGCAGCCAA ACGACGAAGA TGTCGGTGCA ATGTTCAATG CATATGGGTC 
0061 GCTGCTCCAT TTCCACATGG ACGCCACTGC ATGCAGTATC CACTTTCGTA CTATCCACTA 
0121 TCCAGTGTCC CGAACCCCAA GCCCCAAAAC CAGCCTCATC AATTGAGGAA CATGCAAGAA 
0181 CAGCTGCAGC AAGCCGCANC ATCAGCAGGA CGACTCCGAT CTTTGGCTGC ATAATTGCCA 
0241 GTCTAAATCA ATGGGGCGCA AGTTGTTGCC GCTGTGCTGT GCTGTGCTGT GCATGTTTGC 
0301 TGTTGTTGGN GTTGCACCCA TACATGACTC GGTCGGACTT CATTGTCAAC GCCGTTTGCC 
0361 ATTGCCGTTT GcCGTCTCCA TCGGCATCTC TGCATCCACA TTCACATCCA ACTCCATCgN 
0421 CATCGCCATC gNCACCGCCA TCTCCATGTC CCATGTCCCA AGTCTTATGT CCATGTNCGT 
0481 CGCGGTCGTC GTCaATATTT CGC

in silico PCR Fragment

0001 ATGAAGCTGA CAGCAGCCAA ACGACGAAGA TGTCGGTGCA ATGTTCAATG CATATGGGTC 
0061 GCTGCTCCAT TTCCACATGG ACGCCACTGC ATGCAGTATC CACTTTCGTA CTATCCACTA 
0121 TCCAGTGTCC CGAACCCCAA GCCCCAAAAC CAGCCTCATC AATTGAGGAA CATGCAAGAA 
0181 -AGCTGCAGC -AGCCGCAGC ATCAGCAGGA CGACTCCGAT CTTTGGCTGC ATAATTGCCA 
0241 GTCTAAATCA ATGGGGCGCA AGTTGTTGCC GCTGTGCTGT GCTGTGCTGT GCATGTTTGC 
0301 TGTTGTTGGC GTTGCACCCA TACATGACTC GGTCGGACTT CATTGTCAAC GCCGTTTGCC 
0361 ATTGCCGTTT GCCGTCTCCA TCGGCATCTC TGCATCCACA TTCACATCCA ACTCCATCGC 
0421 CATCGCCATC GCCACCGCCA TCTCCATGTC CCATGTCCCA AGTCTTATGT CCATGTCCGT 
0481 CGCGGTCGTC GTCAATATTT -GC

Assemble Data


14532R-3.IR_full       1   ATGAAGCTGACAGCAGCCAAACGACGAAGATGTCGGTGCAATGTTCAATGCATATGGGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     1   ATGAAGCTGACAGCAGCCAAACGACGAAGATGTCGGTGCAATGTTCAATGCATATGGGTC 60


14532R-3.IR_full       61  GCTGCTCCATTTCCACATGGACGCCACTGCATGCAGTATCCACTTTCGTACTATCCACTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     61  GCTGCTCCATTTCCACATGGACGCCACTGCATGCAGTATCCACTTTCGTACTATCCACTA 120


14532R-3.IR_full       121 TCCAGTGTCCCGAACCCCAAGCCCCAAAACCAGCCTCATCAATTGAGGAACATGCAAGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     121 TCCAGTGTCCCGAACCCCAAGCCCCAAAACCAGCCTCATCAATTGAGGAACATGCAAGAA 180


14532R-3.IR_full       181 CAGCTGCAGCAAGCCGCANCATCAGCAGGACGACTCCGATCTTTGGCTGCATAATTGCCA 240
                            ||||||||| ||||||| |||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     181 -AGCTGCAGC-AGCCGCAGCATCAGCAGGACGACTCCGATCTTTGGCTGCATAATTGCCA 240


14532R-3.IR_full       241 GTCTAAATCAATGGGGCGCAAGTTGTTGCCGCTGTGCTGTGCTGTGCTGTGCATGTTTGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     241 GTCTAAATCAATGGGGCGCAAGTTGTTGCCGCTGTGCTGTGCTGTGCTGTGCATGTTTGC 300


14532R-3.IR_full       301 TGTTGTTGGNGTTGCACCCATACATGACTCGGTCGGACTTCATTGTCAACGCCGTTTGCC 360
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
14532R-3.in silico     301 TGTTGTTGGCGTTGCACCCATACATGACTCGGTCGGACTTCATTGTCAACGCCGTTTGCC 360


14532R-3.IR_full       361 ATTGCCGTTTGCCGTCTCCATCGGCATCTCTGCATCCACATTCACATCCAACTCCATCGN 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
14532R-3.in silico     361 ATTGCCGTTTGCCGTCTCCATCGGCATCTCTGCATCCACATTCACATCCAACTCCATCGC 420


14532R-3.IR_full       421 CATCGCCATCGNCACCGCCATCTCCATGTCCCATGTCCCAAGTCTTATGTCCATGTNCGT 480
                           ||||||||||| |||||||||||||||||||||||||||||||||||||||||||| |||
14532R-3.in silico     421 CATCGCCATCGCCACCGCCATCTCCATGTCCCATGTCCCAAGTCTTATGTCCATGTCCGT 480


14532R-3.IR_full       481 CGCGGTCGTCGTCAATATTTCGC 503
                           |||||||||||||||||||| ||
14532R-3.in silico     481 CGCGGTCGTCGTCAATATTT-GC 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	6	30	30	NM_135321.1	CG14532-RA (CG14532), mRNA
1.03	5	6	15	28	NM_137094.1	CG8394-RA (CG8394), mRNA
0.62	3	9	25	61	NM_169851.1	CG31475-RA (CG31475), mRNA
0.62	3	7	21	29	NM_134487.2	CG14222-RA (CG14222), mRNA
0.62	3	7	15	11	NM_136207.2	CG9316-RA (CG9316), mRNA
0.41	2	21	35	49	NM_140223.1	CG14137-RA (CG14137), mRNA
0.41	2	8	17	20	NM_001038758.1	CG9908-RB, transcript variant B (disco), mRNA
0.41	2	8	17	20	NM_078638.2	CG9908-RA, transcript variant A (disco), mRNA
0.41	2	2	16	75	NM_170524.1	CG12072-RA (wts), mRNA
0.2	1	15	42	125	NM_132457.1	CG11122-RA (CG11122), mRNA
0.2	1	7	13	12	NM_136365.1	CG11212-RA (Ptr), mRNA
0.2	1	6	14	35	NM_142710.2	CG16791-RA (CG16791), mRNA
0.2	1	6	4	16	NM_001043256.1	CG34157-RB, transcript variant B (Dys), mRNA
0.2	1	4	12	16	NM_137995.2	CG18426-RA (ytr), mRNA
0.2	1	4	8	15	NM_133042.1	CG12612-RA (CG12612), mRNA
0.2	1	2	11	74	NM_166057.1	CG8787-RA, transcript variant A (Asx), mRNA
0.2	1	2	11	74	NM_001014527.1	CG8787-RB, transcript variant B (Asx), mRNA
0.2	1	2	10	16	NM_168708.1	CG32167-RA (CG32167), mRNA
0.2	1	2	8	15	NM_169286.1	CG9363-RB, transcript variant B (CG9363), mRNA
0.2	1	2	8	15	NM_141638.2	CG9363-RA, transcript variant A (CG9363), mRNA
0.2	1	2	8	15	NM_206468.1	CG9363-RC, transcript variant C (CG9363), mRNA
0.2	1	2	3	10	NM_167777.1	CG14619-RC, transcript variant C (CG14619), mRNA
0	0	10	8	21	NM_144375.2	CG1652-RA (lectin-46Cb), mRNA
0	0	9	30	153	NM_143409.1	CG11873-RA (CG11873), mRNA
0	0	9	28	96	NM_206622.2	CG32776-RB, transcript variant B (CG32776), mRNA
0	0	9	28	96	NM_001038740.1	CG32776-RD, transcript variant D (CG32776), mRNA
0	0	8	40	135	NM_134527.1	CG15322-RA (CG15322), mRNA
0	0	8	7	36	NM_143355.1	CG12852-RA (CG12852), mRNA
0	0	7	18	83	NM_141240.1	CG14656-RA (CG14656), mRNA
0	0	7	12	32	NM_176037.1	CG32972-RB, transcript variant B (CG32972), mRNA

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