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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14450R-3 
 Symbol CG14450  Full Name CG14450 
 CG No CG14450  Old CG No CG14450 
 Synonyms CG14450 
 Accession No (Link to NCBI) NM_141154.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgACCGCTGCAGGAACTCAGCGA 
 Primer Seq. 3'
aatctagaggtaccGGGCCATCAAGGAGAAGCTCA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 ACCGCTGCaG GANCTCAGCG ACATCTCTGT ATGTGGAGGG ATTCGGCACT GAATGGTGAC 
0061 CACCAATGTT CTTTANAGAG TCTGCTTGCG CCGCCGAGCA GTGGGAGCGC ACAAGTAGGG 
0121 GAAAATAGAC CAAAGACTGC ACGAGTCGCG CCCTTTATAA CACCGGCCAT GGACGAGGGA 
0181 CAGCGTTCAC GTATTGTATC TGCTGTAGGG GTGCGCATCG GAGTGACCAG TGTAAGCTGG 
0241 GCGCGATTTA AAGATCGGAA GCAACGAAGC TCCCTGTCTG CTCACGCACT GGCAGCACCA 
0301 TGAGCTGAAC GACAAGAAGC TGCATTTGTC AGAACTTTCC CGCCGATGCC TCTATGTATC 
0361 TCACCAGATA CCCGTGGCCG ATTGCTACGT CATGGAGAGT CCACAAATGG CCCAGGCCAG 
0421 CAGCAACCCA GGGAGCATCG ATCAACAGAA CGTGAACATC CAGAAGGCCC AGGTTTCCGC 
0481 AATCATGAGC TTCTCCTTGA TGGCCC 
 in silico PCR Fragment
0001 ACCGCTGCAG GAACTCAGCG ACATC-CTGT ATGTGGAGGG ATTCGG-ACT GAA-GGTGAC 
0061 CACCAA-GTT CTTTA-AGAG TCTGCTTGCG CCGCCGAGCA GTGGGAGCGC ACAAGTAGGG 
0121 GAAAATAGAC CAAAGACTGC ACGAGTCGCG CCCTTTATAA CACCGGCCAT GGACGAGGGA 
0181 CAGCGTTCAC GTATTGTATC TGCTGTAGGG GTGCGCATCG GAGTGACCAG TGTAAGCTGG 
0241 GCGCGA-TTA AAGATCGGAA GCAACGAAGC TCCCTGTCTG CTCACGCACT GGCAGCACCA 
0301 TGAGCTGAAC GACAAGAAGC TGCATTTGTC AGAACTTTCC CGCCGATGCC TCTATGTATC 
0361 TCACCAGATA CCCGTGGCCG ATTGCTACGT CATGGAGAGT CCACAAATGG CCCAGGCCAG 
0421 CAGCAACCCA GGGAGCATCG ATCAACAGAA CGTGAACATC CAGAAGGCCC AGGTTTCCGC 
0481 AATCATGAGC TTCTCCTTGA TGGCCC 
 Assemble Data

14450R-3.IR_full       1   ACCGCTGCAGGANCTCAGCGACATCTCTGTATGTGGAGGGATTCGGCACTGAATGGTGAC 60
                           |||||||||||| |||||||||||| |||||||||||||||||||| |||||| ||||||
14450R-3.in silico     1   ACCGCTGCAGGAACTCAGCGACATC-CTGTATGTGGAGGGATTCGG-ACTGAA-GGTGAC 60


14450R-3.IR_full       61  CACCAATGTTCTTTANAGAGTCTGCTTGCGCCGCCGAGCAGTGGGAGCGCACAAGTAGGG 120
                           |||||| |||||||| ||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     61  CACCAA-GTTCTTTA-AGAGTCTGCTTGCGCCGCCGAGCAGTGGGAGCGCACAAGTAGGG 120


14450R-3.IR_full       121 GAAAATAGACCAAAGACTGCACGAGTCGCGCCCTTTATAACACCGGCCATGGACGAGGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     121 GAAAATAGACCAAAGACTGCACGAGTCGCGCCCTTTATAACACCGGCCATGGACGAGGGA 180


14450R-3.IR_full       181 CAGCGTTCACGTATTGTATCTGCTGTAGGGGTGCGCATCGGAGTGACCAGTGTAAGCTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     181 CAGCGTTCACGTATTGTATCTGCTGTAGGGGTGCGCATCGGAGTGACCAGTGTAAGCTGG 240


14450R-3.IR_full       241 GCGCGATTTAAAGATCGGAAGCAACGAAGCTCCCTGTCTGCTCACGCACTGGCAGCACCA 300
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     241 GCGCGA-TTAAAGATCGGAAGCAACGAAGCTCCCTGTCTGCTCACGCACTGGCAGCACCA 300


14450R-3.IR_full       301 TGAGCTGAACGACAAGAAGCTGCATTTGTCAGAACTTTCCCGCCGATGCCTCTATGTATC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     301 TGAGCTGAACGACAAGAAGCTGCATTTGTCAGAACTTTCCCGCCGATGCCTCTATGTATC 360


14450R-3.IR_full       361 TCACCAGATACCCGTGGCCGATTGCTACGTCATGGAGAGTCCACAAATGGCCCAGGCCAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     361 TCACCAGATACCCGTGGCCGATTGCTACGTCATGGAGAGTCCACAAATGGCCCAGGCCAG 420


14450R-3.IR_full       421 CAGCAACCCAGGGAGCATCGATCAACAGAACGTGAACATCCAGAAGGCCCAGGTTTCCGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14450R-3.in silico     421 CAGCAACCCAGGGAGCATCGATCAACAGAACGTGAACATCCAGAAGGCCCAGGTTTCCGC 480


14450R-3.IR_full       481 AATCATGAGCTTCTCCTTGATGGCCC 506
                           ||||||||||||||||||||||||||
14450R-3.in silico     481 AATCATGAGCTTCTCCTTGATGGCCC 506

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_141154.2  CG14450-RA (CG14450), mRNA 
0.41   NM_165232.1  CG5674-RB, transcript variant B (CG5674), mRNA 
0.41   NM_136012.4  CG5674-RA, transcript variant A (CG5674), mRNA 
0.41   NM_165233.1  CG5674-RC, transcript variant C (CG5674), mRNA 
0.41   NM_140093.1  CG18178-RA (CG18178), mRNA 
0   NM_170435.1  CG31009-RA (Cad99C), mRNA 
0   NM_164443.1  CG31665-RA, transcript variant A (CG31665), mRNA 
0   NM_205889.1  CG31665-RB, transcript variant B (CG31665), mRNA 
0   NM_145106.1  CG6964-RA, transcript variant A (Gug), mRNA 
0   NM_079249.2  CG6964-RB, transcript variant B (Gug), mRNA 
0   NM_176300.1  CG6964-RC, transcript variant C (Gug), mRNA 
0   NM_206303.1  CG6964-RD, transcript variant D (Gug), mRNA 
0   NM_001043070.1  CG34146-RA (brp), mRNA 
0   NM_080148.2  CG2522-RA (Gtp-bp), mRNA 
0   NM_143279.2  CG6070-RA (CG6070), mRNA 
0   NM_132687.1  CG9941-RA (CG9941), mRNA 
0   NM_136472.3  CG1399-RB, transcript variant B (CG1399), mRNA 
0   NM_165549.2  CG1399-RA, transcript variant A (CG1399), mRNA 
0   NM_080018.1  CG6890-RA (Tollo), mRNA 
0   NM_134926.2  CG3304-RA, transcript variant A (CG3304), mRNA 
0   NM_139864.1  CG8556-RA (Rac2), mRNA 
0   NM_205901.1  CG3304-RC, transcript variant C (CG3304), mRNA 
0   NM_143637.3  CG1937-RA (sip3), mRNA 
0   NM_138238.1  CG13913-RA (CG13913), mRNA 
0   NM_169115.1  CG2017-RB, transcript variant B (CG2017), mRNA 
0   NM_169116.1  CG2017-RC, transcript variant C (CG2017), mRNA 
0   NM_168611.1  CG12316-RB, transcript variant B (CG12316), mRNA 
0   NM_169117.1  CG2017-RD, transcript variant D (CG2017), mRNA 
0   NM_141346.2  CG2017-RA, transcript variant A (CG2017), mRNA 
0   NM_135223.2  CG11199-RA, transcript variant A (Liprin-alpha), mRNA 

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