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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14427R-3 
 Symbol CG14427  Full Name CG14427 
 CG No CG14427  Old CG No CG14427 
 Synonyms CG14427 
 Accession No (Link to NCBI) NM_132151.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGTAGCAGCAGCTCCTCATCG 
 Primer Seq. 3' 
aatctagaggtaccCTCTTGGCCGCACTCTCCATC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GNTAGCAGCA GCTCCTCATC GTCCAGTCGC AGCATCTGGA GCCGCTCCAG CCAGCCGATT 
0061 TGCGATGATT TCGCAGAGCA ACCACAACTG GACAAATCCT GCGGTTACTA CACCTGCAGC 
0121 AGCGCCAATT CATCGCCGAG AAAGGGCCAG GAGAATGACG AGGAGATGGA GGGAGTGCTT 
0181 ATGCGCACGC AGGACATGAG TGCATCATCC CGAGACTCTA CACAATCCGA TCAAATGGTG 
0241 GGCAATCTGG AGTGGGAGAT GCACATGATG CAGCAGGCGC AGCGCATTAT GCCCAAGACC 
0301 TCGTCGCCGA TTTGCCAAAG AAGGGATAAT GCTCAGATGC CTCCCTCATC GTACCAGAAG 
0361 TGGAGAAACT ATCTGCAATC CACGCCAGCG CACATGCTGC ACAATGTCAC CCACATTCCG 
0421 GAGGCAATTG CCGGCCACTT TTGCCCCACC AGCTTTCCGG GCTTCAGCGA CCTGGAGGAG 
0481 ATGGAGAGTG CGGCCAAGAG  
 in silico PCR Fragment 
0001 GGTAGCAGCA GCTCCTCATC GTCCAGTCGC AGCATCTGGA GCCGCTCCAG CCAGCCGATT 
0061 TGCGATGATT TCGCAGAGCA ACCACAACTG GACAAATCCT GCGGTTACTA CACCTGCAGC 
0121 AGCGCCAATT CATCGCCGAG AAAGGGCCAG GAGAATGACG AGGAGATGGA GGGAGTGCTT 
0181 ATGCGCACGC AGGACATGAG TGCATCATCC CGAGACTCTA CACAATCCGA TCAAATGGTG 
0241 GGCAATCTGG AGTGGGAGAT GCACATGATG CAGCAGGCGC AGCGCATTAT GCCCAAGACC 
0301 TCGTCGCCGA TTTGCCAAAG AAGGGATAAT GCTCAGATGC CTCCCTCATC GTACCAGAAG 
0361 TGGAGAAACT ATCTGCAATC CACGCCAGCG CACATGCTGC ACAATGTCAC CCACATTCCG 
0421 GAGGCAATTG CCGGCCACTT TTGCCCCACC AGCTTTCCGG GCTTCAGCGA CCTGGAGGAG 
0481 ATGGAGAGTG CGGCCAAGAG  
 Assemble Data 

14427R-3.IR_full       1   GNTAGCAGCAGCTCCTCATCGTCCAGTCGCAGCATCTGGAGCCGCTCCAGCCAGCCGATT 60
                           | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     1   GGTAGCAGCAGCTCCTCATCGTCCAGTCGCAGCATCTGGAGCCGCTCCAGCCAGCCGATT 60


14427R-3.IR_full       61  TGCGATGATTTCGCAGAGCAACCACAACTGGACAAATCCTGCGGTTACTACACCTGCAGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     61  TGCGATGATTTCGCAGAGCAACCACAACTGGACAAATCCTGCGGTTACTACACCTGCAGC 120


14427R-3.IR_full       121 AGCGCCAATTCATCGCCGAGAAAGGGCCAGGAGAATGACGAGGAGATGGAGGGAGTGCTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     121 AGCGCCAATTCATCGCCGAGAAAGGGCCAGGAGAATGACGAGGAGATGGAGGGAGTGCTT 180


14427R-3.IR_full       181 ATGCGCACGCAGGACATGAGTGCATCATCCCGAGACTCTACACAATCCGATCAAATGGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     181 ATGCGCACGCAGGACATGAGTGCATCATCCCGAGACTCTACACAATCCGATCAAATGGTG 240


14427R-3.IR_full       241 GGCAATCTGGAGTGGGAGATGCACATGATGCAGCAGGCGCAGCGCATTATGCCCAAGACC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     241 GGCAATCTGGAGTGGGAGATGCACATGATGCAGCAGGCGCAGCGCATTATGCCCAAGACC 300


14427R-3.IR_full       301 TCGTCGCCGATTTGCCAAAGAAGGGATAATGCTCAGATGCCTCCCTCATCGTACCAGAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     301 TCGTCGCCGATTTGCCAAAGAAGGGATAATGCTCAGATGCCTCCCTCATCGTACCAGAAG 360


14427R-3.IR_full       361 TGGAGAAACTATCTGCAATCCACGCCAGCGCACATGCTGCACAATGTCACCCACATTCCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     361 TGGAGAAACTATCTGCAATCCACGCCAGCGCACATGCTGCACAATGTCACCCACATTCCG 420


14427R-3.IR_full       421 GAGGCAATTGCCGGCCACTTTTGCCCCACCAGCTTTCCGGGCTTCAGCGACCTGGAGGAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14427R-3.in silico     421 GAGGCAATTGCCGGCCACTTTTGCCCCACCAGCTTTCCGGGCTTCAGCGACCTGGAGGAG 480


14427R-3.IR_full       481 ATGGAGAGTGCGGCCAAGAG 500
                           ||||||||||||||||||||
14427R-3.in silico     481 ATGGAGAGTGCGGCCAAGAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132151.1  CG14427-RA (CG14427), mRNA 
0.2   1  3  5  4  NM_206605.1  CG11579-RE, transcript variant E (arm), mRNA 
0.2   1  3  5  4  NM_134273.2  CG11579-RB, transcript variant B (arm), mRNA 
0.2   1  3  5  4  NM_166912.2  CG11579-RC, transcript variant C (arm), mRNA 
0.2   1  3  5  4  NM_057318.3  CG11579-RA, transcript variant A (arm), mRNA 
0.2   1  3  5  4  NM_057317.3  CG11579-RD, transcript variant D (arm), mRNA 
0.2   1  2  3  4  NM_134658.2  CG11617-RA (CG11617), mRNA 
0   0  4  2  3  NM_079321.2  CG10571-RA (ara), mRNA 
0   0  2  5  7  NM_057685.3  CG11527-RA (Tig), mRNA 
0   0  2  5  2  NM_169578.1  CG3307-RC, transcript variant C (pr-set7), mRNA 
0   0  2  5  2  NM_169577.1  CG3307-RB, transcript variant B (pr-set7), mRNA 
0   0  2  5  2  NM_142097.2  CG3307-RA, transcript variant A (pr-set7), mRNA 
0   0  2  4  6  NM_078699.2  CG1692-RA (mal), mRNA 
0   0  2  3  7  NM_058149.3  CG3352-RA (ft), mRNA 
0   0  2  3  4  NM_136527.1  CG14755-RA (CG14755), mRNA 
0   0  2  2  8  NM_138213.2  CG13897-RA (CG13897), mRNA 
0   0  2  2  3  NM_136431.3  CG11145-RA (CG11145), mRNA 
0   0  2  2  3  NM_141985.1  CG7381-RA, transcript variant A (CG7381), mRNA 
0   0  2  2  3  NM_169485.1  CG7381-RB, transcript variant B (CG7381), mRNA 
0   0  2  2  3  NM_169486.1  CG7381-RC, transcript variant C (CG7381), mRNA 
0   0  2  2  2  NM_080013.2  CG6050-RA (EfTuM), mRNA 
0   0  1  6  3  NM_132380.1  CG15311-RA (CG15311), mRNA 
0   0  1  4  6  NM_140687.4  CG6664-RA, transcript variant A (CG6664), mRNA 
0   0  1  4  6  NM_168709.3  CG6664-RB, transcript variant B (CG6664), mRNA 
0   0  1  4  6  NM_168710.3  CG6664-RC, transcript variant C (CG6664), mRNA 
0   0  1  4  6  NM_168711.2  CG6664-RD, transcript variant D (CG6664), mRNA 
0   0  1  4  4  NM_143493.2  CG31040-RA (Cog7), mRNA 
0   0  1  4  3  NM_078869.4  CG15154-RB, transcript variant B (Socs36E), mRNA 
0   0  1  4  3  NM_165243.1  CG15154-RA, transcript variant A (Socs36E), mRNA 
0   0  1  4  2  NM_168738.1  CG32184-RA (CG32184), mRNA 

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