NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14401R-1

Symbol CG14401 Full Name CG14401

CG No CG14401 Old CG No CG14401

Synonyms CG14401

Accession No (Link to NCBI) NM_136228.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCAGCGCTTGCCTCGCTCTTT

Primer Seq. 3'

aatctagaggtaccGGTCGCCAGTAGGACAAGCAG

Predicted Fragment Size

IR fragment full Seq

0001 GCAGTCGCTT GCCTCGCTCT TTCTGCTCGT CACGCTGGCC AGCTCAGCCC GTGCCATTAC 
0061 CTGCTACGAA TGTGCATTCC GTCAATAATC CAGGGTGTGG GGAGCGATTT GTGGGCGATG 
0121 ACATATCCAC GACGGATTGC GATGTGGTGG CGAACATGCG ATCCCTGGGA GCGGAGGCCA 
0181 CTTGCCTTAC CAAGTACCAT GAGGGAATGC CAGGAGACAC GCGCTTCGTG AGGCGCTCCT 
0241 GTTACTTTGG CGATGCCTCC CCAATAGGCG TCAGTTGCGA CGATGGACCA GATCCTGTGG 
0301 TGCCCTTTAT GAACTTCCTC GGCTgNACAC TGTGCGATAC GGATTTGTGC ANACGCAGCC 
0361 GCTGGATTAT CCACACTACC CTTGGTCATT GCCCTTTCGA TACTCGGACT GCTTGTCCTA 
0421 CTgGNGACC

in silico PCR Fragment

0001 GCAG-CGCTT GCCTCGCTCT TTCTGCTCGT CACGCTGGCC AGCTCAGCCC GTGCCATTAC 
0061 CTGCTACGAA TGTG-ATTCC GTCAATAATC CAGGGTGTGG GGAGCGATTT GTGGGCGATG 
0121 ACATATCCAC GACGGATTGC GATGTGGTGG CGAACATGCG ATCCCTGGGA GCGGAGGCCA 
0181 CTTGCCTTAC CAAGTACCAT GAGGGAATGC CAGGAGACAC GCGCTTCGTG AGGCGCTCCT 
0241 GTTACTTTGG CGATGCCTCC CCAATAGGCG TCAGTTGCGA CGATGGACCA GATCCTGTGG 
0301 TGCCCTTTAT GAACTTCCTC GGCTGCACAC TGTGCGATAC GGATTTGTGC A-ACGCAGCC 
0361 GCTGGATTAT CCACACTACC CTTGGTCATT GCCCTTTCGA TACTCGGACT GCTTGTCCTA 
0421 CTGGCGACC

Assemble Data


14401R-1.IR_full       1   GCAGTCGCTTGCCTCGCTCTTTCTGCTCGTCACGCTGGCCAGCTCAGCCCGTGCCATTAC 60
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     1   GCAG-CGCTTGCCTCGCTCTTTCTGCTCGTCACGCTGGCCAGCTCAGCCCGTGCCATTAC 60


14401R-1.IR_full       61  CTGCTACGAATGTGCATTCCGTCAATAATCCAGGGTGTGGGGAGCGATTTGTGGGCGATG 120
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     61  CTGCTACGAATGTG-ATTCCGTCAATAATCCAGGGTGTGGGGAGCGATTTGTGGGCGATG 120


14401R-1.IR_full       121 ACATATCCACGACGGATTGCGATGTGGTGGCGAACATGCGATCCCTGGGAGCGGAGGCCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     121 ACATATCCACGACGGATTGCGATGTGGTGGCGAACATGCGATCCCTGGGAGCGGAGGCCA 180


14401R-1.IR_full       181 CTTGCCTTACCAAGTACCATGAGGGAATGCCAGGAGACACGCGCTTCGTGAGGCGCTCCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     181 CTTGCCTTACCAAGTACCATGAGGGAATGCCAGGAGACACGCGCTTCGTGAGGCGCTCCT 240


14401R-1.IR_full       241 GTTACTTTGGCGATGCCTCCCCAATAGGCGTCAGTTGCGACGATGGACCAGATCCTGTGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     241 GTTACTTTGGCGATGCCTCCCCAATAGGCGTCAGTTGCGACGATGGACCAGATCCTGTGG 300


14401R-1.IR_full       301 TGCCCTTTATGAACTTCCTCGGCTGNACACTGTGCGATACGGATTTGTGCANACGCAGCC 360
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||| ||||||||
14401R-1.in silico     301 TGCCCTTTATGAACTTCCTCGGCTGCACACTGTGCGATACGGATTTGTGCA-ACGCAGCC 360


14401R-1.IR_full       361 GCTGGATTATCCACACTACCCTTGGTCATTGCCCTTTCGATACTCGGACTGCTTGTCCTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14401R-1.in silico     361 GCTGGATTATCCACACTACCCTTGGTCATTGCCCTTTCGATACTCGGACTGCTTGTCCTA 420


14401R-1.IR_full       421 CTGGNGACC 429
                           |||| ||||
14401R-1.in silico     421 CTGGCGACC 429

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	408	0	0	0	NM_136228.1	CG14401-RA (CG14401), mRNA
0	0	2	2	6	NM_169601.1	CG7425-RA (eff), mRNA
0	0	2	2	2	NM_165989.1	CG30061-RA (CG30061), mRNA
0	0	1	4	2	NM_139499.2	CG2107-RA (CG2107), mRNA
0	0	1	3	5	NM_130557.2	CG14786-RA (CG14786), mRNA
0	0	1	3	2	NM_079470.2	CG10564-RA (Ac78C), mRNA
0	0	1	2	3	NM_141500.1	CG2767-RA (CG2767), mRNA
0	0	1	2	3	NM_140069.2	CG32037-RA (CG32037), mRNA
0	0	1	2	2	NM_167568.2	CG5445-RC, transcript variant C (CG5445), mRNA
0	0	1	2	2	NM_132985.3	CG5445-RA, transcript variant A (CG5445), mRNA
0	0	1	2	2	NM_176748.1	CG5445-RD, transcript variant D (CG5445), mRNA
0	0	1	2	2	NM_167567.1	CG5445-RB, transcript variant B (CG5445), mRNA
0	0	0	4	4	NM_135968.1	CG13280-RA (CG13280), mRNA
0	0	0	4	3	NM_138238.1	CG13913-RA (CG13913), mRNA
0	0	0	4	2	NM_132388.2	CG2909-RA (CG2909), mRNA
0	0	0	3	6	NM_132370.1	CG2989-RA (CG2989), mRNA
0	0	0	3	4	NM_133164.2	CG10142-RA (Ance-5), mRNA
0	0	0	3	4	NM_168310.1	CG5263-RC, transcript variant C (smg), mRNA
0	0	0	3	4	NM_140876.1	CG8798-RA, transcript variant A (CG8798), mRNA
0	0	0	3	4	NM_168806.1	CG8798-RB, transcript variant B (CG8798), mRNA
0	0	0	3	3	NM_132172.2	CG15478-RA (CG15478), mRNA
0	0	0	3	3	NM_137008.1	CG4670-RA (CG4670), mRNA
0	0	0	3	3	NM_057911.3	CG6382-RA (Elf), mRNA
0	0	0	3	3	NM_135553.2	CG5355-RA (CG5355), mRNA
0	0	0	3	2	NM_078952.2	CG4001-RA, transcript variant A (Pfk), mRNA
0	0	0	3	2	NM_165746.1	CG4001-RB, transcript variant B (Pfk), mRNA
0	0	0	3	2	NM_176685.1	CG33080-RA, transcript variant A (CG33080), mRNA
0	0	0	3	2	NM_165747.2	CG4001-RC, transcript variant C (Pfk), mRNA
0	0	0	3	1	NM_168060.1	CG14991-RA, transcript variant A (Fit1), mRNA
0	0	0	3	1	NM_139601.2	CG14991-RB, transcript variant B (Fit1), mRNA

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