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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14362R-1 
 Symbol CG14362  Full Name CG14362 
 CG No CG14362  Old CG No CG14362 
 Synonyms CG14362 
 Accession No (Link to NCBI) NM_142063.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCCTCAGGATGAATACAGCAC 
 Primer Seq. 3' 
aatctagaggtaccGGTCATGTCGAAGACTGCGAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCCTCAGGAT GAATACAGCA CTAAGTCGTA GTCAGATCAA GTATCTGTAT ATTCGATTCC 
0061 ACCAGTTTTC AGGAGGAGGC AAAGATCCCC CAAGTCATCT GCACAAGTAC AATTTCTATT 
0121 CAGGACTACT GCAACTGAAC CCCTTGCTGC CCACCATATT GAACTCCATG TTCGGGAATA 
0181 AAGTGACAAT TACCTTTGTG GATTTCGCTC TGTTTCTGAG CACCTTTCAG GCCCATTCTC 
0241 TTAAAACCTC CAATGAGCTA AAAAATGTGA TGATGGACAA GAAATTAAGG TTAATTTTCA 
0301 ACATGTACGA TAACAACAAG GACGGACGTA TTACCAAGTA TGACTTAGTT GTAGTTGTCC 
0361 ACAAGCTGTT CTCAAATCTA TTGGATCACG TGCAGATAAT GCGCATTGTG AATACCATAA 
0421 TGAAGGAAAT GGATCATACG GACTCGAACC AAATAATGTT CCAAGACTTC TGCAAAgCAT 
0481 TCGCAGTCTT CGACATGACC  
 in silico PCR Fragment 
0001 CCCTCAGGAT GAATACAGCA CTAAGTCGTA GTCAGATCAA GTATCTGTAT ATTCGATTCC 
0061 ACCAGTTTTC AGGAGGAGGC AAAGATCCCC CAAGTCATCT GCACAAGTAC AATTTCTATT 
0121 CAGGACTACT GCAACTAAAC CCCTTGCTGC CCACCATATT GAACTCCATG TTCGGGAATA 
0181 AAGTGACAAT TACCTTTGTG GATTTCGCTC TGTTTCTGAG CACCTTTCAG GCCCATTCTC 
0241 TTAAAACCTC CAATGAGCTA AAAAATGTGA TGATGGACAA GAAATTAAGG TTAATTTTCA 
0301 ACATGTACGA TAACAACAAG GACGGTCGTA TTACAAAGTA TGACTTGGTT GTAGTTGTCC 
0361 ACAAGCTGTT CTCAAATCTA TTGGATCACG TGCAGATAAT GCGCATTGTG AATACCATAA 
0421 TGAAGGAAAT GGATCATACG GACTCGAACC AAATAATGTT CCAAGACTTC TGCAAAGCAT 
0481 TCGCAGTCTT CGACATGACC  
 Assemble Data 

14362R-1.IR_full       1   CCCTCAGGATGAATACAGCACTAAGTCGTAGTCAGATCAAGTATCTGTATATTCGATTCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     1   CCCTCAGGATGAATACAGCACTAAGTCGTAGTCAGATCAAGTATCTGTATATTCGATTCC 60


14362R-1.IR_full       61  ACCAGTTTTCAGGAGGAGGCAAAGATCCCCCAAGTCATCTGCACAAGTACAATTTCTATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     61  ACCAGTTTTCAGGAGGAGGCAAAGATCCCCCAAGTCATCTGCACAAGTACAATTTCTATT 120


14362R-1.IR_full       121 CAGGACTACTGCAACTGAACCCCTTGCTGCCCACCATATTGAACTCCATGTTCGGGAATA 180
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     121 CAGGACTACTGCAACTAAACCCCTTGCTGCCCACCATATTGAACTCCATGTTCGGGAATA 180


14362R-1.IR_full       181 AAGTGACAATTACCTTTGTGGATTTCGCTCTGTTTCTGAGCACCTTTCAGGCCCATTCTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     181 AAGTGACAATTACCTTTGTGGATTTCGCTCTGTTTCTGAGCACCTTTCAGGCCCATTCTC 240


14362R-1.IR_full       241 TTAAAACCTCCAATGAGCTAAAAAATGTGATGATGGACAAGAAATTAAGGTTAATTTTCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     241 TTAAAACCTCCAATGAGCTAAAAAATGTGATGATGGACAAGAAATTAAGGTTAATTTTCA 300


14362R-1.IR_full       301 ACATGTACGATAACAACAAGGACGGACGTATTACCAAGTATGACTTAGTTGTAGTTGTCC 360
                           ||||||||||||||||||||||||| |||||||| ||||||||||| |||||||||||||
14362R-1.in silico     301 ACATGTACGATAACAACAAGGACGGTCGTATTACAAAGTATGACTTGGTTGTAGTTGTCC 360


14362R-1.IR_full       361 ACAAGCTGTTCTCAAATCTATTGGATCACGTGCAGATAATGCGCATTGTGAATACCATAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     361 ACAAGCTGTTCTCAAATCTATTGGATCACGTGCAGATAATGCGCATTGTGAATACCATAA 420


14362R-1.IR_full       421 TGAAGGAAATGGATCATACGGACTCGAACCAAATAATGTTCCAAGACTTCTGCAAAGCAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14362R-1.in silico     421 TGAAGGAAATGGATCATACGGACTCGAACCAAATAATGTTCCAAGACTTCTGCAAAGCAT 480


14362R-1.IR_full       481 TCGCAGTCTTCGACATGACC 500
                           ||||||||||||||||||||
14362R-1.in silico     481 TCGCAGTCTTCGACATGACC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_142063.1  CG14362-RA (CG14362), mRNA 
0   0  3  4  3  NM_134837.2  CG9887-RA (VGlut), mRNA 
0   0  3  3  3  NM_134642.2  CG11371-RB (dbr), mRNA 
0   0  2  6  6  NM_164923.1  CG31716-RE, transcript variant E (CG31716), mRNA 
0   0  2  6  6  NM_164919.1  CG31716-RA, transcript variant A (CG31716), mRNA 
0   0  2  6  6  NM_164921.1  CG31716-RC, transcript variant C (CG31716), mRNA 
0   0  2  6  6  NM_164920.1  CG31716-RB, transcript variant B (CG31716), mRNA 
0   0  2  6  6  NM_164924.1  CG31716-RG, transcript variant G (CG31716), mRNA 
0   0  2  6  6  NM_164922.1  CG31716-RD, transcript variant D (CG31716), mRNA 
0   0  2  5  2  NM_168667.1  CG32159-RB (CG32159), mRNA 
0   0  2  3  2  NM_141206.3  CG9855-RA (CG9855), mRNA 
0   0  2  2  3  NM_080362.2  CG31868-RA (Samuel), mRNA 
0   0  1  5  5  NM_137569.3  CG11961-RB, transcript variant B (CG11961), mRNA 
0   0  1  5  4  NM_166342.1  CG11961-RA, transcript variant A (CG11961), mRNA 
0   0  1  3  3  NM_142961.3  CG6159-RA (sec10), mRNA 
0   0  1  2  7  NM_164887.1  CG31714-RA (CG31714), mRNA 
0   0  1  2  3  NM_132335.2  CG12139-RB (CG12139), mRNA 
0   0  0  7  4  NM_143729.2  CG17342-RA, transcript variant A (Lk6), mRNA 
0   0  0  7  4  NM_169423.2  CG17342-RB, transcript variant B (Lk6), mRNA 
0   0  0  5  3  NM_176548.3  CG33111-RA, transcript variant A (CG33111), mRNA 
0   0  0  5  3  NM_001014646.1  CG33111-RC, transcript variant C (CG33111), mRNA 
0   0  0  5  3  NM_206549.2  CG33111-RB, transcript variant B (CG33111), mRNA 
0   0  0  5  3  NM_165220.1  CG6605-RA (BicD), mRNA 
0   0  0  4  5  NM_206154.1  CG33130-RB, transcript variant B (l(2)k07433), mRNA 
0   0  0  4  5  NM_176218.1  CG33130-RA, transcript variant A (l(2)k07433), mRNA 
0   0  0  4  5  NM_206153.1  CG33130-RC, transcript variant C (l(2)k07433), mRNA 
0   0  0  4  4  NM_142825.2  CG12800-RA (Cyp6d4), mRNA 
0   0  0  4  3  NM_165877.1  CG30203-RA (CG30203), mRNA 
0   0  0  4  3  NM_078602.2  CG32593-RB, transcript variant B (Flo-2), mRNA 
0   0  0  4  3  NM_167416.1  CG32593-RE, transcript variant E (Flo-2), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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