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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
14334R-2 |
| Symbol |
beat-IIa |
Full Name |
beaten path IIa |
| CG No |
CG14334 |
Old CG No |
CG14334 |
| Synonyms |
beat IIa, CT33965, CG14335, CG14334, BcDNA:RE17794, beat-IIa |
| Accession No (Link to NCBI) |
NM_079660.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63
[
PubMed ID = 22417167
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCAGCCGGCGACTTGAGCGAA |
| Primer Seq. 3' |
aatctagaggtaccACTTGCATGGTGTCCACTGCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCAGCCGGCG ACTTGAGCGA AATTCCCAAA ATGGGACACC AAAGGGCCAC GGATACGGGT
0061 ACATCGCCAG ATCCGGAGCC ACAGAAAATC CGACTGCCGG ACTTTTCCAG CCAGATGCCT
0121 CCTGCTGCTA CTCGGCGTTC TACTTCTGAG CATGGAGCTC GTCGAATGCG CGCTGCGCAA
0181 TGNTCAATTT AATAATCGAA CCACCGGCAG TGCGACGGGG CCAGCATGTG GTGCTGCGGT
0241 GCATGTACGA CCTGGACGGG GCTCCACTGT ACTCGGCTAA GTTCTACCGT GGCCAGCTGG
0301 AGTTCTAACC GCTACACGCC CGGCGAGTTT CCCAATACGA AAGTGTTTCC ATTCCCCGGC
0361 ATTCACGTGG ATGTAAGCAG CTCCAATGCC ACCCAGGTGC TGTTGCGCAA CGTGGGATTC
0421 GGTCTCTCTG GCAACTTTTC ATGCGAAGTG ACCGCCGATG CCCCGCTCTT CTCAACCGCC
0481 ACCGCAGTGG ACACCATGCA AGT |
| in silico PCR Fragment |
0001 TCAGCCGGCG ACTTGAGCGA AATTCCCAAA ATGGGACACC AAAGGGCCAC GGATACGGG-
0061 ACATCGCCAG ATCCGGAGCC ACAGAAAATC CGACTGCCGG ACTTTTCCAG CCAGATGCCT
0121 CCTGCTGCTA CTCGGCGTTC TACTTCTGAG CATGGAGCTC GTCGAATGCG CGCTGCGCAA
0181 TG-TCAATTT AATAATCGAA CCACCGGCAG TGCGACGGGG CCAGCATGTG GTGCTGCGGT
0241 GCATGTACGA CCTGGACGGG GCTCCACTGT ACTCGGCTAA GTTCTACCGT GGCCAGCTGG
0301 AGTTCT-ACC GCTACACGCC CGGCGAGTTT CCCAATACGA AAGTGTTTCC ATTCCCCGGC
0361 ATTCACGTGG ATGTAAGCAG CTCCAATGCC ACCCAGGTGC TGTTGCGCAA CGTGGGATTC
0421 GGTCTCTCTG GCAACTTTTC ATGCGAAGTG ACCGCCGATG CCCCGCTCTT CTCAACCGCC
0481 ACCGCAGTGG ACACCATGCA AGT |
| Assemble Data |
14334R-2.IR_full 1 TCAGCCGGCGACTTGAGCGAAATTCCCAAAATGGGACACCAAAGGGCCACGGATACGGGT 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 1 TCAGCCGGCGACTTGAGCGAAATTCCCAAAATGGGACACCAAAGGGCCACGGATACGGG- 60
14334R-2.IR_full 61 ACATCGCCAGATCCGGAGCCACAGAAAATCCGACTGCCGGACTTTTCCAGCCAGATGCCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 61 ACATCGCCAGATCCGGAGCCACAGAAAATCCGACTGCCGGACTTTTCCAGCCAGATGCCT 120
14334R-2.IR_full 121 CCTGCTGCTACTCGGCGTTCTACTTCTGAGCATGGAGCTCGTCGAATGCGCGCTGCGCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 121 CCTGCTGCTACTCGGCGTTCTACTTCTGAGCATGGAGCTCGTCGAATGCGCGCTGCGCAA 180
14334R-2.IR_full 181 TGNTCAATTTAATAATCGAACCACCGGCAGTGCGACGGGGCCAGCATGTGGTGCTGCGGT 240
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 181 TG-TCAATTTAATAATCGAACCACCGGCAGTGCGACGGGGCCAGCATGTGGTGCTGCGGT 240
14334R-2.IR_full 241 GCATGTACGACCTGGACGGGGCTCCACTGTACTCGGCTAAGTTCTACCGTGGCCAGCTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 241 GCATGTACGACCTGGACGGGGCTCCACTGTACTCGGCTAAGTTCTACCGTGGCCAGCTGG 300
14334R-2.IR_full 301 AGTTCTAACCGCTACACGCCCGGCGAGTTTCCCAATACGAAAGTGTTTCCATTCCCCGGC 360
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 301 AGTTCT-ACCGCTACACGCCCGGCGAGTTTCCCAATACGAAAGTGTTTCCATTCCCCGGC 360
14334R-2.IR_full 361 ATTCACGTGGATGTAAGCAGCTCCAATGCCACCCAGGTGCTGTTGCGCAACGTGGGATTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 361 ATTCACGTGGATGTAAGCAGCTCCAATGCCACCCAGGTGCTGTTGCGCAACGTGGGATTC 420
14334R-2.IR_full 421 GGTCTCTCTGGCAACTTTTCATGCGAAGTGACCGCCGATGCCCCGCTCTTCTCAACCGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14334R-2.in silico 421 GGTCTCTCTGGCAACTTTTCATGCGAAGTGACCGCCGATGCCCCGCTCTTCTCAACCGCC 480
14334R-2.IR_full 481 ACCGCAGTGGACACCATGCAAGT 503
|||||||||||||||||||||||
14334R-2.in silico 481 ACCGCAGTGGACACCATGCAAGT 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_079660.3 |
CG14334-RA (beat-IIa), mRNA |
| 0 |
0 |
4 |
5 |
13 |
NM_078855.2 |
CG7644-RA (beat-Ib), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_166688.1 |
CG30426-RA (CG30426), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_142963.2 |
CG6173-RA (kal-1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057393.3 |
CG3158-RA (spn-E), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_136794.1 |
CG13231-RA (CG13231), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_140049.2 |
CG3672-RA (CG3672), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078776.4 |
CG31629-RA, transcript variant A (Pvf3), mRNA |
| 0 |
0 |
1 |
19 |
32 |
NM_142356.2 |
CG4135-RA (beat-IIb), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_132009.2 |
CG11473-RA (CG11473), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_130639.2 |
CG2918-RA (CG2918), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132443.1 |
CG16922-RA (CG16922), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_142717.2 |
CG15498-RA (CG15498), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170365.1 |
CG14066-RC, transcript variant C (larp), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170366.1 |
CG14066-RB, transcript variant B (larp), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080259.1 |
CG14066-RA, transcript variant A (larp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080332.2 |
CG11427-RA (rb), mRNA |
| 0 |
0 |
0 |
8 |
6 |
NM_142714.2 |
CG7922-RA (CG7922), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_135704.2 |
CG5336-RA (Ced-12), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_079007.2 |
CG17716-RA (fas), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_143153.2 |
CG4719-RA (tankyrase), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001042880.1 |
CG31902-RB (CG31902), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_143362.2 |
CG14064-RA (beat-VI), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_166911.3 |
CG3848-RD, transcript variant D (trr), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_080301.2 |
CG3848-RC, transcript variant C (trr), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_080251.2 |
CG12833-RB, transcript variant B (esn), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_165507.1 |
CG12833-RA, transcript variant A (esn), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_165544.1 |
CG2092-RA, transcript variant A (scra), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_165543.1 |
CG2092-RB, transcript variant B (scra), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_137360.1 |
CG6568-RA (CG6568), mRNA |
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