NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14292R-1

Symbol CG14292 Full Name CG14292

CG No CG14292 Old CG No CG14292

Synonyms CG14292

Accession No (Link to NCBI) NM_142502.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATTGTTCTACTGGCGGCCAGC

Primer Seq. 3'

aatctagaggtaccAAACGGGCCTGCCACTCGTCA

Predicted Fragment Size

IR fragment full Seq

0001 NTTGTTCTAC TGGCGGCCAG cATTGCCTGC ATCAGTGCAC AGGGCCCTGG AGNTACTGTT 
0061 GGAAATCCCC TTGCCGGAAA TCCCTTTGCC GCCGCCAATN CCGTATGCGG CTGCCTTTAA 
0121 TCCCTTCCTA AATGGAATTT TCGGAGGTGC TGGCACTGNG AGCTGCCGCC GGTGTAGCCG 
0181 CTCCCGTGGC ACCATCTGCT CCCTTTGGAG GCGCCTCGAT TTCCTCATTC TTCCAGTCGA 
0241 TAGTCCTGCA ACGCGAAGCC GAGCGTCTGC TCTCCCAGCC TGACTTTCCC GCCGACCTGg 
0301 NGGAGCGCGN TCCTGGATGT GGTGGCCACC TCGGAGGAGT CGATCAGTGG CTGCAACAAC 
0361 GCCACTTTGC CCTGGCTGCA GATACGCTGT GTCAAGCCTC TGCTGACCAC GGCCAAGAAC 
0421 CAGCTGAAGG CCATCGATGA CGAGTGGCAG GCCCGTTT

in silico PCR Fragment

0001 ATTGTTCTAC TGGCGGCCAG CATTGCCTGC ATCAGTGCAC AGGGCCCTGG AGCTACTGTT 
0061 GGAAATCCCC TTGCCGGAAA TCCCTTTGCC GCCGCCAAT- CCGTATGCGG CTGCCTTTAA 
0121 TCCCTTCCTA AATGGAATTT TCGGAGGTGC TGGCACAG-G AGCTGCCGCC GGTGTAGCCG 
0181 CTCCCGTGGC ACCGTCTGCT CCCTTTGGAG GCGCCTCGAT TTCCTCATTC TTCCAGTCGA 
0241 TAGTCCTGCA ACGCGAAGCC GAGCGTCTGC TCTCCCAGCC TGACTTTCCC GCCGACCTGG 
0301 CGGAGCGCG- TCCTGGATGT GGTGGCCACC TCGGAGGAGT CGATCAGTGG CTGCAACAAC 
0361 GCCACTTTGC CCTGGCTGCA GATACGCTGT GTCAAGCCTC TGCTGACCAC GGCCAAGAAC 
0421 CAGCTGAAGG CCATCGATGA CGAGTGGCAG GCCCGTTT

Assemble Data


14292R-1.IR_full        1   TTGTTCTACTGGCGGCCAGCATTGCCTGCATCAGTGCACAGGGCCCTGGAGNTACTGTT 59
                            ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
14292R-1.in silico      1   TTGTTCTACTGGCGGCCAGCATTGCCTGCATCAGTGCACAGGGCCCTGGAGCTACTGTT 59


14292R-1.IR_full       61  GGAAATCCCCTTGCCGGAAATCCCTTTGCCGCCGCCAATNCCGTATGCGGCTGCCTTTAA 119
                           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
14292R-1.in silico     61  GGAAATCCCCTTGCCGGAAATCCCTTTGCCGCCGCCAAT-CCGTATGCGGCTGCCTTTAA 119


14292R-1.IR_full       121 TCCCTTCCTAAATGGAATTTTCGGAGGTGCTGGCACTGNGAGCTGCCGCCGGTGTAGCCG 179
                           |||||||||||||||||||||||||||||||||||| | |||||||||||||||||||||
14292R-1.in silico     121 TCCCTTCCTAAATGGAATTTTCGGAGGTGCTGGCACAG-GAGCTGCCGCCGGTGTAGCCG 179


14292R-1.IR_full       181 CTCCCGTGGCACCATCTGCTCCCTTTGGAGGCGCCTCGATTTCCTCATTCTTCCAGTCGA 239
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
14292R-1.in silico     181 CTCCCGTGGCACCGTCTGCTCCCTTTGGAGGCGCCTCGATTTCCTCATTCTTCCAGTCGA 239


14292R-1.IR_full       241 TAGTCCTGCAACGCGAAGCCGAGCGTCTGCTCTCCCAGCCTGACTTTCCCGCCGACCTGG 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14292R-1.in silico     241 TAGTCCTGCAACGCGAAGCCGAGCGTCTGCTCTCCCAGCCTGACTTTCCCGCCGACCTGG 299


14292R-1.IR_full        301 GGAGCGCGNTCCTGGATGTGGTGGCCACCTCGGAGGAGTCGATCAGTGGCTGCAACAAC 358
                            |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
14292R-1.in silico      301 GGAGCGCG-TCCTGGATGTGGTGGCCACCTCGGAGGAGTCGATCAGTGGCTGCAACAAC 358


14292R-1.IR_full       361 GCCACTTTGCCCTGGCTGCAGATACGCTGTGTCAAGCCTCTGCTGACCACGGCCAAGAAC 418
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14292R-1.in silico     361 GCCACTTTGCCCTGGCTGCAGATACGCTGTGTCAAGCCTCTGCTGACCACGGCCAAGAAC 418


14292R-1.IR_full       421 CAGCTGAAGGCCATCGATGACGAGTGGCAGGCCCGTTT 456
                           ||||||||||||||||||||||||||||||||||||||
14292R-1.in silico     421 CAGCTGAAGGCCATCGATGACGAGTGGCAGGCCCGTTT 456

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	437	0	0	4	NM_142502.1	CG14292-RA (CG14292), mRNA
0	0	3	3	2	NM_169896.1	CG4323-RA, transcript variant A (CG4323), mRNA
0	0	3	3	2	NM_169897.1	CG4323-RB, transcript variant B (CG4323), mRNA
0	0	2	5	7	NM_142118.1	CG7987-RA, transcript variant A (CG7987), mRNA
0	0	2	5	7	NM_169587.1	CG7987-RB, transcript variant B (CG7987), mRNA
0	0	2	4	6	NM_132182.1	CG15326-RA (CG15326), mRNA
0	0	2	3	4	NM_135764.3	CG5287-RA (CG5287), mRNA
0	0	2	3	3	NM_132560.2	CG1924-RA (CG1924), mRNA
0	0	2	3	2	NM_141554.2	CG8032-RA (CG8032), mRNA
0	0	2	3	2	NM_143371.1	CG9990-RA, transcript variant A (CG9990), mRNA
0	0	2	3	2	NM_170376.1	CG9990-RB, transcript variant B (CG9990), mRNA
0	0	1	6	10	NM_169301.2	CG31332-RC, transcript variant C (unc-115), mRNA
0	0	1	6	10	NM_169300.2	CG31332-RD, transcript variant D (unc-115), mRNA
0	0	1	6	10	NM_141673.4	CG31352-RA (CG31352), mRNA
0	0	1	6	10	NM_169303.2	CG31332-RB, transcript variant B (unc-115), mRNA
0	0	1	6	10	NM_169302.1	CG31332-RA, transcript variant A (unc-115), mRNA
0	0	1	5	6	NM_166466.2	CG10082-RA, transcript variant A (CG10082), mRNA
0	0	1	5	4	NM_140171.2	CG32075-RA (CG32075), mRNA
0	0	1	5	3	NM_137751.3	CG10082-RB, transcript variant B (CG10082), mRNA
0	0	1	4	2	NM_137754.1	CG15678-RA (CG15678), mRNA
0	0	1	4	2	NM_057316.3	CG5581-RA (Ote), mRNA
0	0	1	3	5	NM_057506.2	CG7672-RA, transcript variant A (gl), mRNA
0	0	1	3	5	NM_169807.2	CG7672-RB, transcript variant B (gl), mRNA
0	0	1	2	4	NM_079850.3	CG1367-RA (CecA2), mRNA
0	0	1	2	3	NM_057970.3	CG6438-RA (amon), mRNA
0	0	1	2	3	NM_139479.2	CG1246-RB (CG1246), mRNA
0	0	1	2	2	NR_002098.1	CG1246-RB (CG1246), mRNA, miscRNA
0	0	1	2	2	NR_002097.1	CG1246-RB (CG1246), mRNA, miscRNA, miscRNA
0	0	0	8	4	NM_057377.3	CG9949-RA, transcript variant A (sina), mRNA
0	0	0	8	4	NM_168689.1	CG9949-RB, transcript variant B (sina), mRNA

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