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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
14260R-4 |
| Symbol |
CG14260 |
Full Name |
CG14260 |
| CG No |
CG14260 |
Old CG No |
CG14260 |
| Synonyms |
CG14260 |
| Accession No (Link to NCBI) |
NM_143294.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Strutt H, Searle E, Thomas-Macarthur V, Brookfield R, Strutt D.
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Development (2013) 140(8) 1693-702
[
PubMed ID = 23487316
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTGTTGCGGAATGGCCAAAA |
| Primer Seq. 3' |
aatctagaggtaccCCTTAACACTCAGCTTGAGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTGTTGCGG AATGGCCAAA AGTCGGACTG CGAGCTCCAT GTCTCGCAAC CGGACAAGTT
0061 GAAGGAGGGC AAGCGTTGTC CATGCATTTT CCGCTGCCAC CAGGTGATGC TNCANTCTCC
0121 GCCTCCGCGG AGTTCGAGCG CCTGATCAGG GATCCCGAGT TCCAGAAGAA CAAGCGAGTG
0181 ATCAGCGTGG ACGACGCCTC TCCCACCGCC TACGAGGcGT TGCTCCTCTA CATCTACACG
0241 TACGAGGTGT GCAATGCGGT GACCATCGAC ATGTGCGGTG ACCTCATGCT GCTGGCCGAG
0301 CGCTACGAGA TGCTGGACTT CATCGACTGC TACATCGACA AGCTGGCCCA CCAGGAGTGG
0361 CCCATGGAGG TGGTTCTGCA GGTCTTCCAC CTGGCCAGCG AGCACCACCA TCCGGCACTG
0421 ATGGATCTCG TGGCGAAAAA AATCCTCCCC GTAGCCACGC AGGTGCTCAT GGACAACTCG
0481 TTCCTCAAGC TGAGTGTTAA GG |
| in silico PCR Fragment |
0001 CCTGTTGCGG AATGGCCAAA AGTCGGACTG CGAGCTCCAT GTCTCGCAAC CGGACAAGTT
0061 GAAGGAGGGC AAGCGTTGTC CATGCATTTT CCGCTGCCAC CAGGTGATGC T-CA-TCTCC
0121 GCCTCCGCGG AGTTCGAGCG CCTGATCAGG GATCCCGAGT TCCAGAAGAA CAAGCGAGTG
0181 ATCAGCGTGG ACGACGCCTC TCCCACCGCC TACGAGGCGT TGCTCCTCTA CATCTACACG
0241 TACGAGGTGT GCAATGCGGT GACCATCGAC ATGTGCGGTG ACCTCATGCT GCTGGCCGAG
0301 CGCTACGAGA TGCTGGACTT CATCGACTGC TACATCGACA AGCTGGCCCA CCAGGAGTGG
0361 CCCATGGAGG TGGTTCTGCA GGTCTTCCAC CTGGCCAGCG AGCACCACCA TCCGGCACTG
0421 ATGGATCTCG TGGCGAAAAA AATCCTCCCC GTAGCCACGC AGGTGCTCAT GGACAACTCG
0481 TTCCTCAAGC TGAGTGTTAA GG |
| Assemble Data |
14260R-4.IR_full 1 CCTGTTGCGGAATGGCCAAAAGTCGGACTGCGAGCTCCATGTCTCGCAACCGGACAAGTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 1 CCTGTTGCGGAATGGCCAAAAGTCGGACTGCGAGCTCCATGTCTCGCAACCGGACAAGTT 60
14260R-4.IR_full 61 GAAGGAGGGCAAGCGTTGTCCATGCATTTTCCGCTGCCACCAGGTGATGCTNCANTCTCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||| || |||||
14260R-4.in silico 61 GAAGGAGGGCAAGCGTTGTCCATGCATTTTCCGCTGCCACCAGGTGATGCT-CA-TCTCC 120
14260R-4.IR_full 121 GCCTCCGCGGAGTTCGAGCGCCTGATCAGGGATCCCGAGTTCCAGAAGAACAAGCGAGTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 121 GCCTCCGCGGAGTTCGAGCGCCTGATCAGGGATCCCGAGTTCCAGAAGAACAAGCGAGTG 180
14260R-4.IR_full 181 ATCAGCGTGGACGACGCCTCTCCCACCGCCTACGAGGCGTTGCTCCTCTACATCTACACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 181 ATCAGCGTGGACGACGCCTCTCCCACCGCCTACGAGGCGTTGCTCCTCTACATCTACACG 240
14260R-4.IR_full 241 TACGAGGTGTGCAATGCGGTGACCATCGACATGTGCGGTGACCTCATGCTGCTGGCCGAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 241 TACGAGGTGTGCAATGCGGTGACCATCGACATGTGCGGTGACCTCATGCTGCTGGCCGAG 300
14260R-4.IR_full 301 CGCTACGAGATGCTGGACTTCATCGACTGCTACATCGACAAGCTGGCCCACCAGGAGTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 301 CGCTACGAGATGCTGGACTTCATCGACTGCTACATCGACAAGCTGGCCCACCAGGAGTGG 360
14260R-4.IR_full 361 CCCATGGAGGTGGTTCTGCAGGTCTTCCACCTGGCCAGCGAGCACCACCATCCGGCACTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 361 CCCATGGAGGTGGTTCTGCAGGTCTTCCACCTGGCCAGCGAGCACCACCATCCGGCACTG 420
14260R-4.IR_full 421 ATGGATCTCGTGGCGAAAAAAATCCTCCCCGTAGCCACGCAGGTGCTCATGGACAACTCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14260R-4.in silico 421 ATGGATCTCGTGGCGAAAAAAATCCTCCCCGTAGCCACGCAGGTGCTCATGGACAACTCG 480
14260R-4.IR_full 481 TTCCTCAAGCTGAGTGTTAAGG 502
||||||||||||||||||||||
14260R-4.in silico 481 TTCCTCAAGCTGAGTGTTAAGG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143294.1 |
CG14260-RA (CG14260), mRNA |
| 0 |
0 |
4 |
5 |
21 |
NM_001042796.1 |
CG6775-RC, transcript variant C (rg), mRNA |
| 0 |
0 |
4 |
5 |
21 |
NM_080023.1 |
CG6775-RA, transcript variant A (rg), mRNA |
| 0 |
0 |
4 |
5 |
21 |
NM_167028.1 |
CG6775-RB, transcript variant B (rg), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_141765.1 |
CG4655-RA, transcript variant A (CG4655), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_142417.1 |
CG7794-RA (CG7794), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_142277.1 |
CG10309-RA (pad), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_167649.1 |
CG32533-RA (CG32533), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_169394.1 |
CG17230-RB, transcript variant B (CG17230), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_141820.1 |
CG17230-RA, transcript variant A (CG17230), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_137960.2 |
CG3957-RA (CG3957), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_134664.1 |
CG17075-RA (CG17075), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132220.2 |
CG2263-RA (CG2263), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168801.1 |
CG9279-RB, transcript variant B (CG9279), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140867.1 |
CG9279-RA, transcript variant A (CG9279), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_137296.2 |
CG8306-RA (CG8306), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_078653.1 |
CG18572-RA, transcript variant A (r), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_140174.4 |
CG7839-RA (CG7839), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140301.1 |
CG11529-RA (CG11529), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_133108.2 |
CG32542-RA (CG32542), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001038952.1 |
CG33975-RA (CG33975), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137507.3 |
CG5341-RA (sec6), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001038986.1 |
CG1721-RC, transcript variant C (Pglym78), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001038987.1 |
CG1721-RB, transcript variant B (Pglym78), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079822.2 |
CG1721-RA, transcript variant A (Pglym78), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169228.1 |
CG31258-RA (CG31258), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014547.1 |
CG3333-RB, transcript variant B (Nop60B), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_080381.2 |
CG3333-RA, transcript variant A (Nop60B), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141399.1 |
CG9727-RA (CG9727), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142732.2 |
CG13415-RA (Cby), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
 |
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JNK signal
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Caspase-3 signal
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