NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14211R-4

Symbol CG14211 Full Name CG14211

CG No CG14211 Old CG No CG14211

Synonyms CG14211

Accession No (Link to NCBI) NM_134488.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15 [ PubMed ID = 25852200 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGAGGTGGACACAGGCCTGTT

Primer Seq. 3'

aatctagaggtaccTCTTGGCCTTGCGCATCTGCT

Predicted Fragment Size

IR fragment full Seq

0001 CGAgGTGGAC ACAGGCCTGT TTCTAGGCAA CCTGACCGCG GCGACGCACA TGGAGACGCT 
0061 GCGCTCCTTC AAGATCACCC ACATCCTCAC GCTGGATTCG GTCCCGCTGC CGCAGCACAT 
0121 TCTGGAGGCC AGCTTTCTGA CCACCAAATA TATACAGATT GCCGACATGC CGCGCGAAGA 
0181 TATTCTGCAG CATCTGGAGG GCTGCGTTGA CTTTATCAGC TCGGCCCTGG cCCAGCAGGG 
0241 CAATGTCCTT GTGCACTGCT ACTTCGGCGT CAGTCGCAGC TCATCCACTG TGATCGCTTA 
0301 CATGATGAAG CGACACAATC TGGACTTCCT GCCCGCCTAC GAGCTGGTCA AGGCCAAACG 
0361 GCGCTTCGTC CAGCCAAACG CTGGCTTTGT CAGCCAACTA AAGCTCTTCC GGCGCATGGG 
0421 CTGCAAGATT GACCCCAACT GCCAGCGCTA CAAGATCCAT CGCCTGCGCC TGGCTGGcGA 
0481 GCAGATGCGC AAGNNCAAGA

in silico PCR Fragment

0001 CGAGGTGGAC ACAGGCCTGT TTCTAGGCAA CCTGACCGCG GCGACGCACA TGGAGACGCT 
0061 GCGCTCCTTC AAGATCACCC ACATCCTCAC GCTGGATTCG GTCCCGCTGC CGCAGCACAT 
0121 TCTGGAGGCC AGCTTTCTGA CCACCAAATA TATACAGATT GCCGACATGC CGCGCGAAGA 
0181 TATTCTGCAG CATCTGGAGG GCTGCGTTGA CTTTATCAGC TCGGCCCTGG CCCAGCAGGG 
0241 CAATGTCCTT GTGCACTGCT ACTTCGGCGT CAGTCGCAGC TCATCCACTG TGATCGCTTA 
0301 CATGATGAAG CGACACAATC TGGACTTCCT GCCCGCCTAC GAGCTGGTCA AGGCCAAACG 
0361 GCGCTTCGTC CAGCCAAACG CTGGCTTTGT CAGCCAACTA AAGCTCTTCC GGCGCATGGG 
0421 CTGCAAGATT GACCCCAACT GCCAGCGCTA CAAGATCCAT CGCCTGCGCC TGGCTGGCGA 
0481 GCAGATGCGC AAGGCCAAGA

Assemble Data


14211R-4.IR_full       1   CGAGGTGGACACAGGCCTGTTTCTAGGCAACCTGACCGCGGCGACGCACATGGAGACGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     1   CGAGGTGGACACAGGCCTGTTTCTAGGCAACCTGACCGCGGCGACGCACATGGAGACGCT 60


14211R-4.IR_full       61  GCGCTCCTTCAAGATCACCCACATCCTCACGCTGGATTCGGTCCCGCTGCCGCAGCACAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     61  GCGCTCCTTCAAGATCACCCACATCCTCACGCTGGATTCGGTCCCGCTGCCGCAGCACAT 120


14211R-4.IR_full       121 TCTGGAGGCCAGCTTTCTGACCACCAAATATATACAGATTGCCGACATGCCGCGCGAAGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     121 TCTGGAGGCCAGCTTTCTGACCACCAAATATATACAGATTGCCGACATGCCGCGCGAAGA 180


14211R-4.IR_full       181 TATTCTGCAGCATCTGGAGGGCTGCGTTGACTTTATCAGCTCGGCCCTGGCCCAGCAGGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     181 TATTCTGCAGCATCTGGAGGGCTGCGTTGACTTTATCAGCTCGGCCCTGGCCCAGCAGGG 240


14211R-4.IR_full       241 CAATGTCCTTGTGCACTGCTACTTCGGCGTCAGTCGCAGCTCATCCACTGTGATCGCTTA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     241 CAATGTCCTTGTGCACTGCTACTTCGGCGTCAGTCGCAGCTCATCCACTGTGATCGCTTA 300


14211R-4.IR_full       301 CATGATGAAGCGACACAATCTGGACTTCCTGCCCGCCTACGAGCTGGTCAAGGCCAAACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     301 CATGATGAAGCGACACAATCTGGACTTCCTGCCCGCCTACGAGCTGGTCAAGGCCAAACG 360


14211R-4.IR_full       361 GCGCTTCGTCCAGCCAAACGCTGGCTTTGTCAGCCAACTAAAGCTCTTCCGGCGCATGGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     361 GCGCTTCGTCCAGCCAAACGCTGGCTTTGTCAGCCAACTAAAGCTCTTCCGGCGCATGGG 420


14211R-4.IR_full       421 CTGCAAGATTGACCCCAACTGCCAGCGCTACAAGATCCATCGCCTGCGCCTGGCTGGCGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14211R-4.in silico     421 CTGCAAGATTGACCCCAACTGCCAGCGCTACAAGATCCATCGCCTGCGCCTGGCTGGCGA 480


14211R-4.IR_full       481 GCAGATGCGCAAGNNCAAGA 500
                           |||||||||||||  |||||
14211R-4.in silico     481 GCAGATGCGCAAGGCCAAGA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_134488.2	CG14211-RB (CG14211), mRNA
0	0	3	3	12	NM_143303.1	CG6599-RA (CG6599), mRNA
0	0	2	5	2	NM_143349.1	CG4869-RA (betaTub97EF), mRNA
0	0	2	4	2	NM_141498.1	CG14463-RA (CG14463), mRNA
0	0	2	3	4	NM_167940.1	CG32305-RA (CG32305), mRNA
0	0	2	2	3	NM_057627.3	CG8996-RB, transcript variant B (wal), mRNA
0	0	2	2	3	NM_165847.1	CG8996-RA, transcript variant A (wal), mRNA
0	0	2	2	2	NM_132054.1	CG6048-RA (CG6048), mRNA
0	0	1	5	12	NM_139680.1	CG17150-RA, transcript variant A (CG17150), mRNA
0	0	1	4	8	NM_137155.2	CG10228-RA (Pcf11), mRNA
0	0	1	3	6	NM_169350.1	CG4067-RB, transcript variant B (pug), mRNA
0	0	1	3	6	NM_001014614.1	CG4067-RD, transcript variant D (pug), mRNA
0	0	1	3	6	NM_057906.3	CG4067-RA, transcript variant A (pug), mRNA
0	0	1	3	6	NM_169351.1	CG4067-RC, transcript variant C (pug), mRNA
0	0	1	3	4	NM_140909.1	CG17736-RA (schuy), mRNA
0	0	1	3	3	NM_206287.1	CG8571-RB, transcript variant B (smid), mRNA
0	0	1	3	3	NM_137713.2	CG4266-RA (CG4266), mRNA
0	0	1	3	3	NM_079235.2	CG8571-RA, transcript variant A (smid), mRNA
0	0	1	3	3	NM_080166.2	CG3766-RA (scat), mRNA
0	0	1	2	8	NM_058124.2	CG5772-RA (Sur), mRNA
0	0	1	2	5	NM_205888.1	CG4629-RC, transcript variant C (CG4629), mRNA
0	0	1	2	5	NM_164404.2	CG4629-RB, transcript variant B (CG4629), mRNA
0	0	1	2	5	NM_134720.2	CG4629-RA, transcript variant A (CG4629), mRNA
0	0	1	2	5	NM_142563.1	CG16718-RA, transcript variant A (CG16718), mRNA
0	0	1	2	4	NM_079002.2	CG3905-RA (Su(z)2), mRNA
0	0	1	2	4	NM_079726.3	CG5374-RA, transcript variant A (T-cp1), mRNA
0	0	1	2	4	NM_142250.1	CG14877-RA (CG14877), mRNA
0	0	1	2	4	NM_170016.1	CG5374-RB, transcript variant B (T-cp1), mRNA
0	0	1	2	3	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	1	2	3	NM_143239.1	CG14250-RA (CG14250), mRNA

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