NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14194R-1

Symbol

CG14194

Full Name

CG14194

CG No

CG14194

Old CG No

CG14194

Synonyms

CG14194

Accession No (Link to NCBI)

NM_133132.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

larval lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGACCGTCACTTGTGGGTGCT

Primer Seq. 3'

aatctagaggtaccTGCCGAACCACCACATGTTGC

Predicted Fragment Size

IR fragment full Seq

0001 GGACCGTCAC TTGTGGGTGC TGGTCTTCAT TCCGCTGATC TTCGGCTCGG TGGTTAGTGT 
0061 GGGCGCCTGT GTCTGGGCCG TCAAGCACGA TCGCTCCTTC GAGCTGGAGC TCTTCCTGGC 
0121 GGTCAACGCG CTTCAGTTTG TTTCACTGCC GCTAAAGCTG GATCGCTTTG TCTACTGGAA 
0181 CTGGGACGTA GTGTTTGTGC CCATGTGGAT CGTCATATGC CTGAGCCTGG TCAGCGTATT 
0241 GTATAACATC ATCTTCTGTG GCATCATGAT GCGTACGCCG GAGGTCTCGC TGCAGCAGAA 
0301 GAAGGCGGCA TTAAACGCAG CGGTGGGCAA CATCTGCACG GTGCTCCCAC TGCTCTGCTT 
0361 TCAGGTGGTC ATCTGCGACA AGCTGGACGG CGAGCTTAAG TTCCCGTACA TCGTGGTCTT 
0421 CTCACCGCTG CTCGTCTCTA TTCTGGCGCT AATAGTTCTT AGCTCCTCGG CAAAGGGCGG 
0481 CAACATGTGG TGGTTCGGCA

in silico PCR Fragment

0001 GGACCGTCAC TTGTGGGTGC TGGTCTTCAT TCCGCTGATC TTCGGCTCGG TGGTTAGTGT 
0061 GGGCGCCTGT GTCTGGGCCG TCAAGCACGA TCGCTCCTTC GAGCTGGAGC TCTTCCTGGC 
0121 GGTCAACGCG CTTCAGTTTG TTTCACTGCC GCTAAAGCTG GATCGCTTTG TCTACTGGAA 
0181 CTGGGACGTA GTGTTTGTGC CCATGTGGAT CGTCATATGC CTGAGCCTGG TCAGCGTATT 
0241 GTATAACATC ATCTTCTGTG GCATCATGAT GCGTACGCCG GAGGTCTCGC TGCAGCAGAA 
0301 GAAGGCGGCA TTAAACGCAG CGGTGGGCAA CATCTGCACG GTGCTCCCAC TGCTCTGCTT 
0361 TCAGGTGGTC ATCTGCGACA AGCTGGACGG CGAGCTTAAG TTCCCGTACA TCGTGGTCTT 
0421 CTCACCGCTG CTCGTCTCTA TTCTGGCGCT AATAGTTCTT AGCTCCTCGG CAAAGGGCGG 
0481 CAACATGTGG TGGTTCGGCA

Assemble Data


14194R-1.IR_full       1   GGACCGTCACTTGTGGGTGCTGGTCTTCATTCCGCTGATCTTCGGCTCGGTGGTTAGTGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     1   GGACCGTCACTTGTGGGTGCTGGTCTTCATTCCGCTGATCTTCGGCTCGGTGGTTAGTGT 60


14194R-1.IR_full       61  GGGCGCCTGTGTCTGGGCCGTCAAGCACGATCGCTCCTTCGAGCTGGAGCTCTTCCTGGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     61  GGGCGCCTGTGTCTGGGCCGTCAAGCACGATCGCTCCTTCGAGCTGGAGCTCTTCCTGGC 120


14194R-1.IR_full       121 GGTCAACGCGCTTCAGTTTGTTTCACTGCCGCTAAAGCTGGATCGCTTTGTCTACTGGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     121 GGTCAACGCGCTTCAGTTTGTTTCACTGCCGCTAAAGCTGGATCGCTTTGTCTACTGGAA 180


14194R-1.IR_full       181 CTGGGACGTAGTGTTTGTGCCCATGTGGATCGTCATATGCCTGAGCCTGGTCAGCGTATT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     181 CTGGGACGTAGTGTTTGTGCCCATGTGGATCGTCATATGCCTGAGCCTGGTCAGCGTATT 240


14194R-1.IR_full       241 GTATAACATCATCTTCTGTGGCATCATGATGCGTACGCCGGAGGTCTCGCTGCAGCAGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     241 GTATAACATCATCTTCTGTGGCATCATGATGCGTACGCCGGAGGTCTCGCTGCAGCAGAA 300


14194R-1.IR_full       301 GAAGGCGGCATTAAACGCAGCGGTGGGCAACATCTGCACGGTGCTCCCACTGCTCTGCTT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     301 GAAGGCGGCATTAAACGCAGCGGTGGGCAACATCTGCACGGTGCTCCCACTGCTCTGCTT 360


14194R-1.IR_full       361 TCAGGTGGTCATCTGCGACAAGCTGGACGGCGAGCTTAAGTTCCCGTACATCGTGGTCTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     361 TCAGGTGGTCATCTGCGACAAGCTGGACGGCGAGCTTAAGTTCCCGTACATCGTGGTCTT 420


14194R-1.IR_full       421 CTCACCGCTGCTCGTCTCTATTCTGGCGCTAATAGTTCTTAGCTCCTCGGCAAAGGGCGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14194R-1.in silico     421 CTCACCGCTGCTCGTCTCTATTCTGGCGCTAATAGTTCTTAGCTCCTCGGCAAAGGGCGG 480


14194R-1.IR_full       481 CAACATGTGGTGGTTCGGCA 500
                           ||||||||||||||||||||
14194R-1.in silico     481 CAACATGTGGTGGTTCGGCA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_133132.1	CG14194-RA (CG14194), mRNA
0	0	3	3	4	NM_133076.2	CG6470-RA (CG6470), mRNA
0	0	3	2	2	NM_057267.3	CG9579-RA (AnnX), mRNA
0	0	2	2	5	NM_001014616.1	CG33512-RA (dpr4), mRNA
0	0	1	6	3	NM_130544.3	CG14622-RA, transcript variant A (DAAM), mRNA
0	0	1	6	3	NM_166875.1	CG14622-RC, transcript variant C (DAAM), mRNA
0	0	1	6	3	NM_166876.1	CG14622-RB, transcript variant B (DAAM), mRNA
0	0	1	4	6	NM_143790.1	CG11538-RA (CG11538), mRNA
0	0	1	4	6	NM_080077.2	CG6806-RA (Lsp2), mRNA
0	0	1	4	3	NM_135571.1	CG13144-RA (CG13144), mRNA
0	0	0	6	3	NM_141659.2	CG8286-RA (CG8286), mRNA
0	0	0	6	3	NM_141755.2	CG14691-RA, transcript variant A (CG14691), mRNA
0	0	0	6	3	NM_176456.1	CG14691-RB, transcript variant B (CG14691), mRNA
0	0	0	5	11	NM_132068.1	CG15899-RB (Ca-alpha1T), mRNA
0	0	0	4	15	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0	0	0	4	8	NM_134601.1	CG1718-RA (CG1718), mRNA
0	0	0	4	6	NM_057938.3	CG3093-RA (dor), mRNA
0	0	0	4	6	NM_167398.1	CG32599-RA (CG32599), mRNA
0	0	0	4	5	NM_132403.3	CG17255-RA, transcript variant A (CG17255), mRNA
0	0	0	4	5	NM_167231.3	CG17255-RB, transcript variant B (CG17255), mRNA
0	0	0	4	4	NM_001042820.1	CG34145-RA (CG34145), mRNA
0	0	0	4	3	NM_137202.2	CG8190-RA (eIF2B-gamma), mRNA
0	0	0	4	3	NM_206589.1	CG1883-RD, transcript variant D (RpS7), mRNA
0	0	0	4	3	NM_132836.1	CG8206-RA (CG8206), mRNA
0	0	0	4	3	NM_170476.1	CG1883-RC, transcript variant C (RpS7), mRNA
0	0	0	4	3	NM_143525.3	CG1883-RA, transcript variant A (RpS7), mRNA
0	0	0	4	3	NM_176752.1	CG6835-RC, transcript variant C (GS), mRNA
0	0	0	4	3	NM_176753.1	CG6835-RD, transcript variant D (GS), mRNA
0	0	0	4	3	NM_170477.2	CG1883-RB, transcript variant B (RpS7), mRNA
0	0	0	4	3	NM_140688.2	CG7728-RA (CG7728), mRNA

Back