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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14110R-1 
 Symbol CG14110  Full Name CG14110 
 CG No CG14110  Old CG No CG14110 
 Synonyms CG14110 
 Accession No (Link to NCBI) NM_140381.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTTCTCACCATTCAAGCTGC 
 Primer Seq. 3' 
aatctagaggtaccTGGTTGGAAGGCTCAACGAAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGTTCTCACC ATTCAAGCTG CTCCTGTCGA GGTAACCGAA GGAGTGACCC AACCCCCAGC 
0061 CGAGAAGCTG CAAACGGAGT CTCGTTCCCG TAAAGATGAA GAGCTGGAAC TAGAAGAAGA 
0121 CCTGGATGGA CGTAATGCCT ATATCAACAA TCAGTTTGTG CCAAGCGAAC CTGCAGAAGT 
0181 CCTAGAGGAT GTGCAGAATA CGCCGCTCTA CGAGATCCTT CAAAAGCAGA TGGAACAAGT 
0241 GTTGGCCTCA TCGGCTCCCA ATGACCCTGT CTACGAGCAG CACCGCGAGN AAGCAGGATC 
0301 GTCGCGATCA GAAACCACCA CACCAGCCAC CGTACTTCTC CGGCGGAGAT CGAGATGGTG 
0361 ATCTACAGGA TGACTACGAG GACGAGGAGG ATCAGACGGA TCAGGGTTAT CCGTCGGACC 
0421 CCGAGAAACC GGACGCCGTG TCAGATGCTC CCGAGGATGA GGACCCAGGC TACCAGGCAC 
0481 CTTCGTTGAG CCTTCCAACC A 
 in silico PCR Fragment 
0001 TGTTCTCACC ATTCAAGCTG CTCCTGTCGA GGTAACCGAA GGAGTGACCC AACCCCCAGC 
0061 CGAGAAGCTG CAAACGGAGT CTCGTTCCCG TAAAGATGAA GAGCTGGAAC TAGAAGAAGA 
0121 CCTGGATGGA CGTAATGCCT ATATCAACAA TCAGTTTGTG CCAAGCGAAC CTGCAGAAGT 
0181 CCTAGAGGAT GTGCAGAATA CGCCGCTCTA CGAGATCCTT CAAAAGCAGA TGGAACAAGT 
0241 GTTGGCCTCA TCGGCTCCCA ATGACCCTGT CTACGAGCAG CACCGCGAG- AAGCAGGATC 
0301 GTCGCGATCA GAAACCACCA CACCAGCCAC CGTACTTCTC CGGCGGAGAT CGAGATGGTG 
0361 ATCTACAGGA TGACTACGAG GACGAGGAGG ATCAGACGGA TCAGGGTTAT CCGTCGGACC 
0421 CCGAGAAACC GGACGCCGTG TCAGATGCTC CCGAGGATGA GGACCCAGGC TACCAGGCAC 
0481 CTTCGTTGAG CCTTCCAACC A 
 Assemble Data 

14110R-1.IR_full       1   TGTTCTCACCATTCAAGCTGCTCCTGTCGAGGTAACCGAAGGAGTGACCCAACCCCCAGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     1   TGTTCTCACCATTCAAGCTGCTCCTGTCGAGGTAACCGAAGGAGTGACCCAACCCCCAGC 60


14110R-1.IR_full       61  CGAGAAGCTGCAAACGGAGTCTCGTTCCCGTAAAGATGAAGAGCTGGAACTAGAAGAAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     61  CGAGAAGCTGCAAACGGAGTCTCGTTCCCGTAAAGATGAAGAGCTGGAACTAGAAGAAGA 120


14110R-1.IR_full       121 CCTGGATGGACGTAATGCCTATATCAACAATCAGTTTGTGCCAAGCGAACCTGCAGAAGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     121 CCTGGATGGACGTAATGCCTATATCAACAATCAGTTTGTGCCAAGCGAACCTGCAGAAGT 180


14110R-1.IR_full       181 CCTAGAGGATGTGCAGAATACGCCGCTCTACGAGATCCTTCAAAAGCAGATGGAACAAGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     181 CCTAGAGGATGTGCAGAATACGCCGCTCTACGAGATCCTTCAAAAGCAGATGGAACAAGT 240


14110R-1.IR_full       241 GTTGGCCTCATCGGCTCCCAATGACCCTGTCTACGAGCAGCACCGCGAGNAAGCAGGATC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
14110R-1.in silico     241 GTTGGCCTCATCGGCTCCCAATGACCCTGTCTACGAGCAGCACCGCGAG-AAGCAGGATC 300


14110R-1.IR_full       301 GTCGCGATCAGAAACCACCACACCAGCCACCGTACTTCTCCGGCGGAGATCGAGATGGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     301 GTCGCGATCAGAAACCACCACACCAGCCACCGTACTTCTCCGGCGGAGATCGAGATGGTG 360


14110R-1.IR_full       361 ATCTACAGGATGACTACGAGGACGAGGAGGATCAGACGGATCAGGGTTATCCGTCGGACC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     361 ATCTACAGGATGACTACGAGGACGAGGAGGATCAGACGGATCAGGGTTATCCGTCGGACC 420


14110R-1.IR_full       421 CCGAGAAACCGGACGCCGTGTCAGATGCTCCCGAGGATGAGGACCCAGGCTACCAGGCAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14110R-1.in silico     421 CCGAGAAACCGGACGCCGTGTCAGATGCTCCCGAGGATGAGGACCCAGGCTACCAGGCAC 480


14110R-1.IR_full       481 CTTCGTTGAGCCTTCCAACCA 501
                           |||||||||||||||||||||
14110R-1.in silico     481 CTTCGTTGAGCCTTCCAACCA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140381.1  CG14110-RA (CG14110), mRNA 
0   0  6  10  8  NM_165089.2  CG3479-RA, transcript variant A (osp), mRNA 
0   0  6  10  7  NM_078843.4  CG3479-RB, transcript variant B (osp), mRNA 
0   0  5  4  5  NM_136687.2  CG1472-RA (sec24), mRNA 
0   0  4  2  3  NM_166968.1  CG32498-RN, transcript variant N (dnc), mRNA 
0   0  4  2  3  NM_166970.1  CG32498-RG, transcript variant G (dnc), mRNA 
0   0  3  6  3  NM_176242.1  CG33133-RA (grau), mRNA 
0   0  3  5  5  NM_134768.3  CG17660-RA (CG17660), mRNA 
0   0  2  6  13  NM_142899.1  CG10152-RA (beat-IV), mRNA 
0   0  2  4  8  NM_169486.1  CG7381-RC, transcript variant C (CG7381), mRNA 
0   0  2  4  8  NM_141985.1  CG7381-RA, transcript variant A (CG7381), mRNA 
0   0  2  4  8  NM_169485.1  CG7381-RB, transcript variant B (CG7381), mRNA 
0   0  2  4  6  NM_142538.1  CG5237-RA (CG5237), mRNA 
0   0  2  3  7  NM_001014741.1  CG11111-RC, transcript variant C (rdgB), mRNA 
0   0  2  3  7  NM_167382.1  CG11111-RA, transcript variant A (rdgB), mRNA 
0   0  2  3  7  NM_001014740.1  CG11111-RD, transcript variant D (rdgB), mRNA 
0   0  2  3  7  NM_078594.2  CG11111-RB, transcript variant B (rdgB), mRNA 
0   0  2  3  6  NM_139849.2  CG8598-RA, transcript variant A (eco), mRNA 
0   0  2  3  6  NM_168201.2  CG8598-RB, transcript variant B (eco), mRNA 
0   0  2  3  3  NM_001038815.1  CG17988-RB (Ance-3), mRNA 
0   0  2  3  3  NM_137344.2  CG30456-RA (CG30456), mRNA 
0   0  2  3  3  NM_142229.2  CG4525-RA (CG4525), mRNA 
0   0  2  2  4  NM_135247.2  CG9138-RA (SP1070), mRNA 
0   0  2  2  2  NM_001042801.1  CG34104-RB, transcript variant B (CG34104), mRNA 
0   0  2  2  2  NM_001042802.1  CG34104-RA, transcript variant A (CG34104), mRNA 
0   0  1  8  19  NM_132413.1  CG15295-RA (CG15295), mRNA 
0   0  1  8  6  NM_079967.4  CG6611-RA, transcript variant A (ect), mRNA 
0   0  1  8  6  NM_168400.1  CG6611-RB, transcript variant B (ect), mRNA 
0   0  1  8  6  NM_168401.1  CG6611-RC, transcript variant C (ect), mRNA 
0   0  1  5  2  NM_164430.2  CG31666-RD, transcript variant D (CG31666), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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