NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14086R-2

Symbol CG14086 Full Name CG14086

CG No CG14086 Old CG No CG14086

Synonyms CG14086

Accession No (Link to NCBI) NM_140845.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTTGTGACTATCTTCCTGC

Primer Seq. 3'

aatctagaggtaccCAAACATCGTCGTCTAGGGCG

Predicted Fragment Size

IR fragment full Seq

0001 GGCTTGTGAC TATCTTCCTG CAACTTCAGT GCGCAGAGGA TAATTACCAG CGAAGGTCCT 
0061 GCAGCTGCTT GGCCTGCTGG CGACTTTCAG CGATTTGTGG CGGTTGGCGA CCCACTCCTG 
0121 TTTATGCGGT CATCGGCATC TGCCTGATCC TGTATCCCCA CAATCTGTGG CTGAGTTATG 
0181 TGGCCGGGAT GTTCCTGATA CTCCTCGGAC TCCTCAGACT GTGCACGCTG CTCCGATTCA 
0241 GCGCCTCGAA GGAGGAGGGT CTCCTGCCGC AGTGCGACTT TGAAAAGGTG TCGAGCTTTG 
0301 TGAATACCAT GGAGGATGAT TTCGCGGAGA TTGGTGCCAC GCAAGCGGGA TTGGATGAGG 
0361 AAGCGGAGGA GGAGGGCGAC GACCACACCG CCCTAGACGA CGATGTTTG

in silico PCR Fragment

0001 GGCTTGTGAC TATCTTCCTG CAACTTCAGT GCGCAGAGGA TAATTACCAG CGAAGGTCCT 
0061 GCAGCTGCTT GGCCTGCTGG CGACTTTCAG CGATTTGTGG CGGTTGGCGA CCCACTCCTG 
0121 TTTATGCGGT CATCGGCATC TGCCTGATCC TGTATCCCCA CAATCTGTGG CTGAGTTATG 
0181 TGGCCGGGAT GTTCCTGATA CTCCTCGGAC TCCTCAGACT GTGCACGCTG CTCCGATTCA 
0241 GCGCCTCGAA GGAGGAGGGT CTCCTGCCGC AGTGCGACTT TGAAAAGGTG TCGAGCTTTG 
0301 TGGATACCAT GGAGGATGAT TTCGCGGAGA TTGGTGCCAC GCAAGCGGGA TTGGATGAGG 
0361 AAGCGGAGGA GGAGGGCGAC GACCACACCG CCCTAGACGA CGATGTTTG

Assemble Data


14086R-2.IR_full       1   GGCTTGTGACTATCTTCCTGCAACTTCAGTGCGCAGAGGATAATTACCAGCGAAGGTCCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     1   GGCTTGTGACTATCTTCCTGCAACTTCAGTGCGCAGAGGATAATTACCAGCGAAGGTCCT 60


14086R-2.IR_full       61  GCAGCTGCTTGGCCTGCTGGCGACTTTCAGCGATTTGTGGCGGTTGGCGACCCACTCCTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     61  GCAGCTGCTTGGCCTGCTGGCGACTTTCAGCGATTTGTGGCGGTTGGCGACCCACTCCTG 120


14086R-2.IR_full       121 TTTATGCGGTCATCGGCATCTGCCTGATCCTGTATCCCCACAATCTGTGGCTGAGTTATG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     121 TTTATGCGGTCATCGGCATCTGCCTGATCCTGTATCCCCACAATCTGTGGCTGAGTTATG 180


14086R-2.IR_full       181 TGGCCGGGATGTTCCTGATACTCCTCGGACTCCTCAGACTGTGCACGCTGCTCCGATTCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     181 TGGCCGGGATGTTCCTGATACTCCTCGGACTCCTCAGACTGTGCACGCTGCTCCGATTCA 240


14086R-2.IR_full       241 GCGCCTCGAAGGAGGAGGGTCTCCTGCCGCAGTGCGACTTTGAAAAGGTGTCGAGCTTTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     241 GCGCCTCGAAGGAGGAGGGTCTCCTGCCGCAGTGCGACTTTGAAAAGGTGTCGAGCTTTG 300


14086R-2.IR_full       301 TGAATACCATGGAGGATGATTTCGCGGAGATTGGTGCCACGCAAGCGGGATTGGATGAGG 360
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     301 TGGATACCATGGAGGATGATTTCGCGGAGATTGGTGCCACGCAAGCGGGATTGGATGAGG 360


14086R-2.IR_full       361 AAGCGGAGGAGGAGGGCGACGACCACACCGCCCTAGACGACGATGTTTG 409
                           |||||||||||||||||||||||||||||||||||||||||||||||||
14086R-2.in silico     361 AAGCGGAGGAGGAGGGCGACGACCACACCGCCCTAGACGACGATGTTTG 409

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	391	0	0	0	NM_140845.1	CG14086-RA (CG14086), mRNA
0.25	1	5	2	4	NM_135694.2	CG6785-RA (CG6785), mRNA
0.25	1	4	4	15	NM_135939.2	CG4132-RA (pkaap), mRNA
0.25	1	3	4	6	NM_134318.1	CG10327-RC, transcript variant C (TBPH), mRNA
0.25	1	3	4	6	NM_166628.1	CG10327-RD, transcript variant D (TBPH), mRNA
0.25	1	3	4	6	NM_058052.2	CG10327-RB, transcript variant B (TBPH), mRNA
0.25	1	3	4	5	NM_058051.2	CG10327-RA, transcript variant A (TBPH), mRNA
0.25	1	2	9	20	NM_139852.2	CG8591-RA (CTCF), mRNA
0.25	1	2	3	8	NM_169654.2	CG31302-RC, transcript variant C (CG31302), mRNA
0.25	1	2	3	7	NM_169652.2	CG31302-RA, transcript variant A (CG31302), mRNA
0.25	1	2	3	7	NM_169653.2	CG31302-RB, transcript variant B (CG31302), mRNA
0	0	5	2	3	NM_135357.3	CG8183-RA, transcript variant A (Khc-73), mRNA
0	0	5	2	3	NM_176176.1	CG8183-RB, transcript variant B (Khc-73), mRNA
0	0	4	2	2	NM_166114.2	CG30087-RA (CG30087), mRNA
0	0	3	4	10	NM_138247.1	CG9134-RB, transcript variant B (CG9134), mRNA
0	0	3	4	3	NM_143207.1	CG14556-RA (CG14556), mRNA
0	0	3	3	6	NM_206254.1	CG12002-RD, transcript variant D (Pxn), mRNA
0	0	3	3	6	NM_206253.1	CG12002-RE, transcript variant E (Pxn), mRNA
0	0	3	3	6	NM_206255.1	CG12002-RC, transcript variant C (Pxn), mRNA
0	0	3	3	6	NM_079167.3	CG12002-RA, transcript variant A (Pxn), mRNA
0	0	3	3	2	NM_167957.1	CG12002-RB, transcript variant B (Pxn), mRNA
0	0	2	21	47	NM_167375.1	CG7107-RB, transcript variant B (up), mRNA
0	0	2	21	47	NM_001014738.1	CG7107-RF, transcript variant F (up), mRNA
0	0	2	21	47	NM_001014737.1	CG7107-RG, transcript variant G (up), mRNA
0	0	2	21	47	NM_001014739.1	CG7107-RE, transcript variant E (up), mRNA
0	0	2	21	47	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
0	0	2	21	47	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
0	0	2	11	9	NM_139725.2	CG5249-RA (Blimp-1), mRNA
0	0	2	5	8	NM_143196.1	CG8968-RA (CG8968), mRNA
0	0	2	3	5	NM_169501.1	CG8644-RA (Osi22), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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