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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1406R-1 |
| Symbol |
U2A |
Full Name |
U2A |
| CG No |
CG1406 |
Old CG No |
CG1406 |
| Synonyms |
U2A', l(2)43Ee, dU2A', CG1406, BcDNA:RE56869, U2A |
| Accession No (Link to NCBI) |
NM_136471.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAAGTGCCTGCTGCTGAACAA |
| Primer Seq. 3' |
aatctagaggtaccGCAGGATCTGCGATAGACGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAAGTGCCTG CTGCTGAACA ACAACCGCAT CCTGCGCATC AGCGAAGGGC TGGAGGAGGC
0061 GGTGcCCAAC CTGGGCTCCA TCATTCTCAC CGGCAACAAC CTGCAGGAGC TGAGCGACTT
0121 NAGAGCCCCT GGTCGGATTC ACCAAGCTGG AAACCATCTG CCTGCTTATC AACCCGGTAT
0181 CCACGAAGCC CAACTACCGC GAGTACATGG CCTACAAGTT CCCGCAGCTG NGGCTTACTC
0241 GACTTCAGGA AGATCAAGCA GAAGGACCGC CAGGCGGCGC AGGTAGTTCT TCCGCACCAA
0301 GCAGGGCAAG GACGTGCTAA AGGAGATTAG CCGGAAGTCC AAGATGAGCG CCGCAGCCGC
0361 CATAGCTGCA GAGGCGGGGN AATGGCAAGG GAAGGGGATC GGAAGGCGGA CGACTGGCGA
0421 ATCCGCAGGA CATGCAgcGC NATCCGCGAG GCCATCAAGC GGGCCAGCTC TCTGGCAGAG
0481 GTGGAGCGTC TANTCGCAGG TCCTGGGTAC C |
| in silico PCR Fragment |
0001 CAAGTGCCTG CTGCTGAACA ACAACCGCAT CCTGCGCATC AGCGAAGGGC TGGAGGAGGC
0061 GGTGCCCAAC CTGGGCTCCA TCATTCTCAC CGGCAACAAC CTGCAGGAGC TGAGCGACTT
0121 -AGAGCCCCT GGTCGGATTC ACCAAGCTGG AAACCATCTG CCTGCTTATC AACCCGGTAT
0181 CCACGAAGCC CAACTACCGC GAGTACATGG CCTACAAGTT CCCGCAGCTG CGGC-TACTC
0241 GACTTCAGGA AGATCAAGCA GAAGGACCGC CAGGCGGCGC AGG-AGTTCT TCCGCACCAA
0301 GCAGGGCAAG GACGTGCTAA AGGAGATTAG CCGGAAGTCC AAGATGAGCG CCGCAGCCGC
0361 CATAGCTGCA GAGGCGGGG- AATGGCAAGG GAAGGGGATC GGAAGGCGGA CGACTGGCCA
0421 ATCCGCAGGA CATGCAGCGC -ATCCGCGAG GCCATCAAGC GGGCCAGCTC TCTGGCAGAG
0481 GTGGAGCGTC TA-TCGCAGA TCCTG----- C |
| Assemble Data |
1406R-1.IR_full 1 CAAGTGCCTGCTGCTGAACAACAACCGCATCCTGCGCATCAGCGAAGGGCTGGAGGAGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1406R-1.in silico 1 CAAGTGCCTGCTGCTGAACAACAACCGCATCCTGCGCATCAGCGAAGGGCTGGAGGAGGC 60
1406R-1.IR_full 61 GGTGCCCAACCTGGGCTCCATCATTCTCACCGGCAACAACCTGCAGGAGCTGAGCGACTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1406R-1.in silico 61 GGTGCCCAACCTGGGCTCCATCATTCTCACCGGCAACAACCTGCAGGAGCTGAGCGACTT 120
1406R-1.IR_full 121 AGAGCCCCTGGTCGGATTCACCAAGCTGGAAACCATCTGCCTGCTTATCAACCCGGTAT 179
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1406R-1.in silico 121 AGAGCCCCTGGTCGGATTCACCAAGCTGGAAACCATCTGCCTGCTTATCAACCCGGTAT 179
1406R-1.IR_full 181 CCACGAAGCCCAACTACCGCGAGTACATGGCCTACAAGTTCCCGCAGCTGNGGCTTACTC 239
|||||||||||||||||||||||||||||||||||||||||||||||||| ||| |||||
1406R-1.in silico 181 CCACGAAGCCCAACTACCGCGAGTACATGGCCTACAAGTTCCCGCAGCTGCGGC-TACTC 239
1406R-1.IR_full 241 GACTTCAGGAAGATCAAGCAGAAGGACCGCCAGGCGGCGCAGGTAGTTCTTCCGCACCAA 299
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
1406R-1.in silico 241 GACTTCAGGAAGATCAAGCAGAAGGACCGCCAGGCGGCGCAGG-AGTTCTTCCGCACCAA 299
1406R-1.IR_full 301 GCAGGGCAAGGACGTGCTAAAGGAGATTAGCCGGAAGTCCAAGATGAGCGCCGCAGCCGC 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1406R-1.in silico 301 GCAGGGCAAGGACGTGCTAAAGGAGATTAGCCGGAAGTCCAAGATGAGCGCCGCAGCCGC 359
1406R-1.IR_full 361 CATAGCTGCAGAGGCGGGGNAATGGCAAGGGAAGGGGATCGGAAGGCGGACGACTGGCGA 419
||||||||||||||||||| |||||||||||||||||||||||||||||||||||||| |
1406R-1.in silico 361 CATAGCTGCAGAGGCGGGG-AATGGCAAGGGAAGGGGATCGGAAGGCGGACGACTGGCCA 419
1406R-1.IR_full 421 ATCCGCAGGACATGCAGCGCNATCCGCGAGGCCATCAAGCGGGCCAGCTCTCTGGCAGAG 479
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
1406R-1.in silico 421 ATCCGCAGGACATGCAGCGC-ATCCGCGAGGCCATCAAGCGGGCCAGCTCTCTGGCAGAG 479
1406R-1.IR_full 481 GTGGAGCGTCTANTCGCAGGTCCTGGGTACC 510
|||||||||||| |||||| ||||| |
1406R-1.in silico 481 GTGGAGCGTCTA-TCGCAGATCCTG-----C 510
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_136471.2 |
CG1406-RA (U2A), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_132018.2 |
CG3149-RA (CG3149), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_080182.2 |
CG9156-RA (Pp1-13C), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_132538.1 |
CG15737-RA (CG15737), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_001043241.1 |
CG14713-RB (CG14713), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_143395.1 |
CG1443-RA (CG1443), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_138202.2 |
CG17183-RA (MED30), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_137742.2 |
CG10494-RB, transcript variant B (CG10494), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_166457.1 |
CG10494-RA, transcript variant A (CG10494), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_136574.2 |
CG8258-RA (CG8258), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_136891.2 |
CG8877-RA (prp8), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_166792.1 |
CG11153-RA, transcript variant A (Sox102F), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_135235.2 |
CG10805-RA (CG10805), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001014695.1 |
CG11153-RB, transcript variant B (Sox102F), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_169999.1 |
CG7084-RB, transcript variant B (CG7084), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142759.1 |
CG7084-RA, transcript variant A (CG7084), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_130557.2 |
CG14786-RA (CG14786), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168087.1 |
CG32241-RA (CG32241), mRNA |
| 0 |
0 |
1 |
3 |
13 |
NM_164655.2 |
CG7235-RB, transcript variant B (Hsp60C), mRNA |
| 0 |
0 |
1 |
3 |
13 |
NM_135104.4 |
CG7235-RC, transcript variant C (Hsp60C), mRNA |
| 0 |
0 |
1 |
3 |
13 |
NM_164654.1 |
CG7235-RA, transcript variant A (Hsp60C), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_079167.3 |
CG12002-RA, transcript variant A (Pxn), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_206255.1 |
CG12002-RC, transcript variant C (Pxn), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_206254.1 |
CG12002-RD, transcript variant D (Pxn), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_206253.1 |
CG12002-RE, transcript variant E (Pxn), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134727.2 |
CG4577-RA (CG4577), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167705.1 |
CG1695-RB, transcript variant B (CG1695), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134550.1 |
CG1695-RA, transcript variant A (CG1695), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057534.3 |
CG3242-RA (sob), mRNA |
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