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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14043R-3 
 Symbol CG14043  Full Name CG14043 
 CG No CG14043  Old CG No CG14043 
 Synonyms CG14043 
 Accession No (Link to NCBI) NM_135042.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTCCAAAGGAGATCCTAAGCG 
 Primer Seq. 3' 
aatctagaggtaccGAACACATCTTGAATGGCCAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTCCAAAGGA GATCCTAAGC GACTTCAAGG AGCTATACGA GAAGGCCAAC CGTCTGGTGG 
0061 AGGAGGAGAG CCGCAATGAT CCGCCCACGG ATCCATTCCG CTCCCATTAT AAAGCGCGCG 
0121 ATGTGCTAAT TGTCTTGAAG AAGCAGCTGG ACGACCAACT GGTATCTGTG CAGGCCAGCG 
0181 AGGAGGACGG TGGTCAGGAC GATCGCTGCT ATCACTCTCT GTTGGCCTTC GTTTGCCGGG 
0241 ATCTAGGCAG GATCTACATC TACACAGAGG AGCAGGCAGA GGGCGAAAAG ATGCTCAATC 
0301 GTTGTCTGGA ACTAGTCACG CCGTTTAAGG AGTGCCCTGA GGGCATCNAT TCCATTCATT 
0361 GGGGCAATCA ACGAGCTTAG CATTGTGCTG GCCTCCAAGG AAGAGTACAA TAAGGGACTG 
0421 GAAATTCTCC TGGAGGCAGA AAAAATCTAC GAGGACTTCA AAGCCAGTGG TCTGAAGCCC 
0481 CTGGCCATTC AAGATGTGTT C 
 in silico PCR Fragment 
0001 TTCCAAAGGA GATCCTAAGC GACTTCAAGG AGCTATACGA GAAGGCCAAC CGTCTGGTGG 
0061 AGGAGGAGAG CCGCAATGAT CCGCCCACGG ATCCATTCCG CTCCCATTAT AAAGCGCGCG 
0121 ATGTGCTAAT TGTCTTGAAG AAGCAGCTGG ACGACCAACT GGTATCTGTG CAGGCCAGCG 
0181 AGGAGGACGG TGGTCAGGAC GATCGCTGCT ATCACTCTCT GTTGGCCTTC GTTTGCCGGG 
0241 ATCTAGGCAG GATCTACATC TACACAGAGG AGCAGGCAGA GGGCGAAAAG ATGCTCAATC 
0301 GTTGTCTGGA ACTAGTCACG CCGTTTAAGG AGTGCCCTGA GGGCATC-AT TCCATTCATT 
0361 GGGGCAATCA ACGAGCTTAG CATTGTGCTG GCCTCCAAGG AAGAGTACAA TAAGGGACTG 
0421 GAAATTCTCC TGGAGGCAGA AAAAATCTAC GAGGACTTCA AAGCCAGTGG TCTGAAGCCC 
0481 CTGGCCATTC AAGATGTGTT C 
 Assemble Data 

14043R-3.IR_full       1   TTCCAAAGGAGATCCTAAGCGACTTCAAGGAGCTATACGAGAAGGCCAACCGTCTGGTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     1   TTCCAAAGGAGATCCTAAGCGACTTCAAGGAGCTATACGAGAAGGCCAACCGTCTGGTGG 60


14043R-3.IR_full       61  AGGAGGAGAGCCGCAATGATCCGCCCACGGATCCATTCCGCTCCCATTATAAAGCGCGCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     61  AGGAGGAGAGCCGCAATGATCCGCCCACGGATCCATTCCGCTCCCATTATAAAGCGCGCG 120


14043R-3.IR_full       121 ATGTGCTAATTGTCTTGAAGAAGCAGCTGGACGACCAACTGGTATCTGTGCAGGCCAGCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     121 ATGTGCTAATTGTCTTGAAGAAGCAGCTGGACGACCAACTGGTATCTGTGCAGGCCAGCG 180


14043R-3.IR_full       181 AGGAGGACGGTGGTCAGGACGATCGCTGCTATCACTCTCTGTTGGCCTTCGTTTGCCGGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     181 AGGAGGACGGTGGTCAGGACGATCGCTGCTATCACTCTCTGTTGGCCTTCGTTTGCCGGG 240


14043R-3.IR_full       241 ATCTAGGCAGGATCTACATCTACACAGAGGAGCAGGCAGAGGGCGAAAAGATGCTCAATC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     241 ATCTAGGCAGGATCTACATCTACACAGAGGAGCAGGCAGAGGGCGAAAAGATGCTCAATC 300


14043R-3.IR_full       301 GTTGTCTGGAACTAGTCACGCCGTTTAAGGAGTGCCCTGAGGGCATCNATTCCATTCATT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
14043R-3.in silico     301 GTTGTCTGGAACTAGTCACGCCGTTTAAGGAGTGCCCTGAGGGCATC-ATTCCATTCATT 360


14043R-3.IR_full       361 GGGGCAATCAACGAGCTTAGCATTGTGCTGGCCTCCAAGGAAGAGTACAATAAGGGACTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     361 GGGGCAATCAACGAGCTTAGCATTGTGCTGGCCTCCAAGGAAGAGTACAATAAGGGACTG 420


14043R-3.IR_full       421 GAAATTCTCCTGGAGGCAGAAAAAATCTACGAGGACTTCAAAGCCAGTGGTCTGAAGCCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14043R-3.in silico     421 GAAATTCTCCTGGAGGCAGAAAAAATCTACGAGGACTTCAAAGCCAGTGGTCTGAAGCCC 480


14043R-3.IR_full       481 CTGGCCATTCAAGATGTGTTC 501
                           |||||||||||||||||||||
14043R-3.in silico     481 CTGGCCATTCAAGATGTGTTC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135042.2  CG14043-RA (CG14043), mRNA 
0   0  4  5  2  NM_135140.2  CG12393-RA, transcript variant A (CG12393), mRNA 
0   0  4  5  2  NM_164678.1  CG12393-RB, transcript variant B (CG12393), mRNA 
0   0  3  5  3  NM_176712.1  CG33181-RA (CG33181), mRNA 
0   0  2  4  8  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  2  4  8  NM_079009.2  CG18076-RA, transcript variant A (shot), mRNA 
0   0  2  4  8  NM_166017.1  CG18076-RE, transcript variant E (shot), mRNA 
0   0  2  4  8  NM_166016.1  CG18076-RB, transcript variant B (shot), mRNA 
0   0  2  4  8  NM_166015.1  CG18076-RG, transcript variant G (shot), mRNA 
0   0  2  4  7  NM_166018.1  CG18076-RC, transcript variant C (shot), mRNA 
0   0  1  3  4  NM_139808.1  CG10064-RA (CG10064), mRNA 
0   0  1  2  3  NM_137876.1  CG30264-RA (CG30264), mRNA 
0   0  1  2  3  NM_166317.1  CG18608-RA (prod), mRNA 
0   0  1  2  3  NM_141799.1  CG17187-RA (CG17187), mRNA 
0   0  0  7  9  NM_139778.2  CG10274-RA (CG10274), mRNA 
0   0  0  6  3  NM_057242.3  CG7765-RA (Khc), mRNA 
0   0  0  6  3  NM_138259.2  CG9165-RA (l(3)02640), mRNA 
0   0  0  5  14  NM_166533.1  CG6339-RA (rad50), mRNA 
0   0  0  5  9  NM_057737.2  CG15804-RA, transcript variant A (Dhc62B), mRNA 
0   0  0  5  9  NM_206236.1  CG15804-RB, transcript variant B (Dhc62B), mRNA 
0   0  0  5  7  NM_164799.1  CG8409-RB, transcript variant B (Su(var)205), mRNA 
0   0  0  5  7  NM_057407.2  CG8409-RA, transcript variant A (Su(var)205), mRNA 
0   0  0  5  6  NM_058035.2  CG8717-RA (slv), mRNA 
0   0  0  5  5  NM_164857.1  CG3811-RA, transcript variant A (Oatp30B), mRNA 
0   0  0  5  5  NM_205946.1  CG3811-RC, transcript variant C (Oatp30B), mRNA 
0   0  0  5  5  NM_135449.2  CG3811-RB, transcript variant B (Oatp30B), mRNA 
0   0  0  5  5  NM_205945.1  CG3811-RD, transcript variant D (Oatp30B), mRNA 
0   0  0  5  5  NM_164832.1  CG9280-RC, transcript variant C (Glt), mRNA 
0   0  0  5  5  NM_164831.1  CG9280-RB, transcript variant B (Glt), mRNA 
0   0  0  5  5  NM_058156.2  CG9280-RA, transcript variant A (Glt), mRNA 

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