NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14032R-4

Symbol

Cyp4ac1

Full Name

Cyp4ac1

CG No

CG14032

Old CG No

CG14032

Synonyms

CG14032, 4ac1, Cyp4ac1

Accession No (Link to NCBI)

NM_135072.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATAATGTGGTTCGACCCGAG

Primer Seq. 3'

aatctagaggtaccATGCGACTGGAGAAGTCGTGA

Predicted Fragment Size

IR fragment full Seq

0001 TATAATGTGG TTCGACCCGA GGAAGCTGAG GAGGTATTCC AGAGCACCAA GCTGATAACG 
0061 AAGAACGTGG TTTACGAACT GATTAGACCA TTTCTGGGAG ACGGACTCCT GATAAGCACG 
0121 GATCACAAAT GGCATTCCAG ACGAAAGGCT TTAACGCCCG CCTTTCACTT CAATGTCCTG 
0181 CAGTCATTCC TGGGTATTTT CAAGGAAGAG TGCAAGAAGT TCCTTAACGT TTTAGAGAAA 
0241 AATTTAGATG CAGAACTGGA GCTTAACCAG GTTATTCCGC CATTTACTTT GAATAATATA 
0301 TGCGAAACTG CTCTGGGTGT GAAGCTGGAT GACATGTCGG AGGGCAACGA ATATAGAAAG 
0361 GCCATCCATG CCATCGAGGA AGTTCTAATT CAACGCGTGT GCAATCCGCT GATGTACTAC 
0421 AACTGGTATT TCTTCGTTTA CGGAGACTAT AGAAAGCATT TGCAGAATCT TCGAATAGTT 
0481 CACGACTTCT CCAGTCGCAT

in silico PCR Fragment

0001 TATAATGTGG TTCGACCCGA GGAAGCTGAG GAGGTATTCC AGAGCACCAA GCTGATAACG 
0061 AAGAACGTGG TTTACGAACT GATTAGACCA TTTCTGGGAG ACGGACTCCT GATAAGCACG 
0121 GATCACAAAT GGCATTCCAG ACGAAAGGCT TTAACGCCCG CCTTTCACTT CAATGTCCTG 
0181 CAGTCATTCC TGGGTATTTT CAAGGAAGAG TGCAAGAAGT TCCTTAACGT TTTAGAGAAA 
0241 AATTTAGATG CAGAACTGGA GCTTAACCAG GTTATTCCGC CATTTACTTT GAATAATATA 
0301 TGCGAAACTG CTCTGGGTGT GAAGCTGGAT GACATGTCGG AGGGCAACGA ATATAGAAAG 
0361 GCCATCCATG CCATCGAGGA AGTTCTAATT CAACGCGTGT GCAATCCGCT GATGTACTAC 
0421 AACTGGTATT TCTTCGTTTA CGGAGACTAT AGAAAGCATT TGCAGAATCT TCGAATAGTT 
0481 CACGACTTCT CCAGTCGCAT

Assemble Data


14032R-4.IR_full       1   TATAATGTGGTTCGACCCGAGGAAGCTGAGGAGGTATTCCAGAGCACCAAGCTGATAACG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     1   TATAATGTGGTTCGACCCGAGGAAGCTGAGGAGGTATTCCAGAGCACCAAGCTGATAACG 60


14032R-4.IR_full       61  AAGAACGTGGTTTACGAACTGATTAGACCATTTCTGGGAGACGGACTCCTGATAAGCACG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     61  AAGAACGTGGTTTACGAACTGATTAGACCATTTCTGGGAGACGGACTCCTGATAAGCACG 120


14032R-4.IR_full       121 GATCACAAATGGCATTCCAGACGAAAGGCTTTAACGCCCGCCTTTCACTTCAATGTCCTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     121 GATCACAAATGGCATTCCAGACGAAAGGCTTTAACGCCCGCCTTTCACTTCAATGTCCTG 180


14032R-4.IR_full       181 CAGTCATTCCTGGGTATTTTCAAGGAAGAGTGCAAGAAGTTCCTTAACGTTTTAGAGAAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     181 CAGTCATTCCTGGGTATTTTCAAGGAAGAGTGCAAGAAGTTCCTTAACGTTTTAGAGAAA 240


14032R-4.IR_full       241 AATTTAGATGCAGAACTGGAGCTTAACCAGGTTATTCCGCCATTTACTTTGAATAATATA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     241 AATTTAGATGCAGAACTGGAGCTTAACCAGGTTATTCCGCCATTTACTTTGAATAATATA 300


14032R-4.IR_full       301 TGCGAAACTGCTCTGGGTGTGAAGCTGGATGACATGTCGGAGGGCAACGAATATAGAAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     301 TGCGAAACTGCTCTGGGTGTGAAGCTGGATGACATGTCGGAGGGCAACGAATATAGAAAG 360


14032R-4.IR_full       361 GCCATCCATGCCATCGAGGAAGTTCTAATTCAACGCGTGTGCAATCCGCTGATGTACTAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     361 GCCATCCATGCCATCGAGGAAGTTCTAATTCAACGCGTGTGCAATCCGCTGATGTACTAC 420


14032R-4.IR_full       421 AACTGGTATTTCTTCGTTTACGGAGACTATAGAAAGCATTTGCAGAATCTTCGAATAGTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14032R-4.in silico     421 AACTGGTATTTCTTCGTTTACGGAGACTATAGAAAGCATTTGCAGAATCTTCGAATAGTT 480


14032R-4.IR_full       481 CACGACTTCTCCAGTCGCAT 500
                           ||||||||||||||||||||
14032R-4.in silico     481 CACGACTTCTCCAGTCGCAT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135072.2	CG14032-RA (Cyp4ac1), mRNA
0	0	3	50	83	NM_135074.2	CG14031-RA (Cyp4ac3), mRNA
0	0	3	3	6	NM_079979.2	CG18497-RB, transcript variant B (spen), mRNA
0	0	3	3	6	NM_164374.1	CG18497-RA, transcript variant A (spen), mRNA
0	0	3	3	6	NM_164375.1	CG18497-RC, transcript variant C (spen), mRNA
0	0	2	5	11	NM_057559.3	CG3656-RA, transcript variant A (Cyp4d1), mRNA
0	0	2	2	4	NM_132026.1	CG4078-RA (CG4078), mRNA
0	0	1	7	6	NM_136629.2	CG10843-RA (Cyp4p3), mRNA
0	0	1	3	4	NM_080148.2	CG2522-RA (Gtp-bp), mRNA
0	0	1	2	7	NM_141557.1	CG8043-RA (CG8043), mRNA
0	0	1	2	3	NM_132182.1	CG15326-RA (CG15326), mRNA
0	0	0	11	30	NM_135073.2	CG17970-RA (Cyp4ac2), mRNA
0	0	0	6	7	NM_144078.1	CG18676-RA (Teh3), mRNA
0	0	0	6	5	NM_144278.2	CG15345-RA (CG15345), mRNA
0	0	0	6	5	NM_001043297.1	CG3339-RB, transcript variant B (CG3339), mRNA
0	0	0	6	5	NM_143300.1	CG3339-RA, transcript variant A (CG3339), mRNA
0	0	0	5	8	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	0	5	8	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	0	5	8	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	0	5	6	NM_080305.2	CG10755-RA (Cyp4ae1), mRNA
0	0	0	5	4	NM_136518.3	CG2183-RA (CG2183), mRNA
0	0	0	4	8	NM_132493.2	CG11715-RA, transcript variant A (Cyp4g15), mRNA
0	0	0	4	4	NM_206179.1	CG7753-RA (mei-W68), mRNA
0	0	0	4	4	NM_001043217.1	CG34113-RP, transcript variant P (CG34113), mRNA
0	0	0	4	4	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	0	4	3	NM_135959.1	CG17996-RA (CG17996), mRNA
0	0	0	4	2	NM_139560.2	CG14971-RA (CG14971), mRNA
0	0	0	3	17	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	3	8	NM_079572.2	CG9484-RA (hyd), mRNA
0	0	0	3	5	NM_168297.1	CG32026-RA (CG32026), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Back