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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13868R-2 
 Symbol CG13868  Full Name CG13868 
 CG No CG13868  Old CG No CG13868 
 Synonyms anon-WO0153538.60, CG13868 
 Accession No (Link to NCBI) NM_137628.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATGAGGATGAAGAGGCCACT 
 Primer Seq. 3' 
aatctagaggtaccTCATACTGCCAGACATTGTCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATGAGGATG AAGAGGCCAC TgccGCCGNC GCTGCCCACG ATCCGTTGCT CCCGGCGGAT 
0061 CAGCAGCTGG CGGATGCGAT GGTGCGCGGA ATGGGATTGG GATTGACTAC GACGCGATCT 
0121 GATTTATCCG CAACTGAGCA CCCAACTGCA CATGGCACTG GCGCCATTCG TCGCACACCC 
0181 NTCCACCTCC AGCGAGGAGG AGGAAGAGGA GGAGGACCTC GAGGACCACG AGCTGGTGCC 
0241 TTCGCCCGAT CTGACATCGG TGGATAGATC GGAGCGTTAC TCCGACATCA GGCAGCTGTT 
0301 GGCCAGACAG CAGCCCGTCG CAGCTGCTCT GGAGGGCATC TCCGGCGCTC AATGGATAGT 
0361 GGGCGACGGC TATGACCTGG AGGCCTTCAA TCAGGTGAAC GGAGTGTGGC CCCTGGGTCA 
0421 TCCGCTGCCG CTGCCCGACT GGTCGAGCTC GGAGTNCGCT GGCAAGGGCA CCCTGATCTT 
0481 CGACAATGTC TGGCAGTATG A 
 in silico PCR Fragment 
0001 GATGAGGATG AAGAGGCCAC TGCCGCCGCC GCTGCCCACG ATCCGTTGCT CCCGGCGGAT 
0061 CAGCAGCTGG CGGATGCGAT GGTGCGCGGA ATGGGATTGG GATTGACTAC GACGCGATCT 
0121 GATTTATCCG CAACTGAGCA CCCAACTGCA CATGGCACTG GCGCCATTCG TCGCACACCC 
0181 -TCCACCTCC AGCGAGGAGG AGGAAGAGGA GGAGGACCTC GAGGACCACG AGCTGGTGCC 
0241 TTCGCCCGAT CTGACATCGG TGGATAGATC GGAGCGTTAC TCCGACATCA GGCAGCTGTT 
0301 GGCCAGACAG CAGCCCGTCG CAGCTGCTCT GGAGGGCATC TCCGGCGCTC AATGGATAGT 
0361 GGGCGACGGC TATGACCTAG AGGCCTTCAA TCAGGTAAAC GGAGTGTGGC CCCTGGGTCA 
0421 TCCGCTGCCG CTGCCCGACT GGTCGAGCTC GGAGTCCGCT GGCAAGGGCA CCCTGATCTT 
0481 CGACAATGTC TGGCAGTATG A 
 Assemble Data 

13868R-2.IR_full        1   ATGAGGATGAAGAGGCCACTGCCGCCGNCGCTGCCCACGATCCGTTGCTCCCGGCGGAT 59
                            ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
13868R-2.in silico      1   ATGAGGATGAAGAGGCCACTGCCGCCGCCGCTGCCCACGATCCGTTGCTCCCGGCGGAT 59


13868R-2.IR_full       61  CAGCAGCTGGCGGATGCGATGGTGCGCGGAATGGGATTGGGATTGACTACGACGCGATCT 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13868R-2.in silico     61  CAGCAGCTGGCGGATGCGATGGTGCGCGGAATGGGATTGGGATTGACTACGACGCGATCT 119


13868R-2.IR_full       121 GATTTATCCGCAACTGAGCACCCAACTGCACATGGCACTGGCGCCATTCGTCGCACACCC 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13868R-2.in silico     121 GATTTATCCGCAACTGAGCACCCAACTGCACATGGCACTGGCGCCATTCGTCGCACACCC 179


13868R-2.IR_full        181 TCCACCTCCAGCGAGGAGGAGGAAGAGGAGGAGGACCTCGAGGACCACGAGCTGGTGCC 238
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13868R-2.in silico      181 TCCACCTCCAGCGAGGAGGAGGAAGAGGAGGAGGACCTCGAGGACCACGAGCTGGTGCC 238


13868R-2.IR_full       241 TTCGCCCGATCTGACATCGGTGGATAGATCGGAGCGTTACTCCGACATCAGGCAGCTGTT 298
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13868R-2.in silico     241 TTCGCCCGATCTGACATCGGTGGATAGATCGGAGCGTTACTCCGACATCAGGCAGCTGTT 298


13868R-2.IR_full       301 GGCCAGACAGCAGCCCGTCGCAGCTGCTCTGGAGGGCATCTCCGGCGCTCAATGGATAGT 358
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13868R-2.in silico     301 GGCCAGACAGCAGCCCGTCGCAGCTGCTCTGGAGGGCATCTCCGGCGCTCAATGGATAGT 358


13868R-2.IR_full       361 GGGCGACGGCTATGACCTGGAGGCCTTCAATCAGGTGAACGGAGTGTGGCCCCTGGGTCA 418
                           |||||||||||||||||| ||||||||||||||||| |||||||||||||||||||||||
13868R-2.in silico     361 GGGCGACGGCTATGACCTAGAGGCCTTCAATCAGGTAAACGGAGTGTGGCCCCTGGGTCA 418


13868R-2.IR_full       421 TCCGCTGCCGCTGCCCGACTGGTCGAGCTCGGAGTNCGCTGGCAAGGGCACCCTGATCTT 478
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
13868R-2.in silico     421 TCCGCTGCCGCTGCCCGACTGGTCGAGCTCGGAGTCCGCTGGCAAGGGCACCCTGATCTT 478


13868R-2.IR_full       481 CGACAATGTCTGGCAGTATGA 499
                           |||||||||||||||||||||
13868R-2.in silico     481 CGACAATGTCTGGCAGTATGA 499



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  4  6  NM_137628.1  CG13868-RA (CG13868), mRNA 
1.45   7  19  84  96  NM_001014738.1  CG7107-RF, transcript variant F (up), mRNA 
1.45   7  19  84  94  NM_001014737.1  CG7107-RG, transcript variant G (up), mRNA 
1.45   7  19  84  94  NM_080349.2  CG7107-RA, transcript variant A (up), mRNA 
1.45   7  19  84  94  NM_167375.1  CG7107-RB, transcript variant B (up), mRNA 
1.45   7  19  84  94  NM_167376.1  CG7107-RD, transcript variant D (up), mRNA 
1.45   7  19  84  92  NM_001014739.1  CG7107-RE, transcript variant E (up), mRNA 
1.03   5  2  22  23  NM_143060.1  CG13647-RA (CG13647), mRNA 
1.03   5  2  14  47  NM_176185.1  CG8421-RE, transcript variant E (Asph), mRNA 
1.03   5  2  14  47  NM_166141.2  CG8421-RD, transcript variant D (Asph), mRNA 
1.03   5  2  12  27  NM_057951.2  CG9210-RA, transcript variant A (Ac13E), mRNA 
1.03   5  2  12  27  NM_001014745.1  CG9210-RB, transcript variant B (Ac13E), mRNA 
1.03   5  2  10  37  NM_166140.1  CG8421-RA, transcript variant A (Asph), mRNA 
1.03   5  2  10  37  NM_079033.2  CG8421-RB, transcript variant B (Asph), mRNA 
0.82   4  2  4  3  NM_132817.1  CG9215-RA (CG9215), mRNA 
0.62   3  3  4  22  NM_001014630.1  CG33547-RA (Rim), mRNA 
0.41   2  20  23  70  NM_133114.2  CG32541-RA (CG32541), mRNA 
0.41   2  11  50  64  NM_132012.1  CG15776-RA (CG15776), mRNA 
0.41   2  5  34  59  NM_132346.1  CG3003-RB (CG3003), mRNA 
0.41   2  5  6  15  NM_168099.1  CG32234-RA (CG32234), mRNA 
0.41   2  4  20  44  NM_170139.1  CG31132-RA (BRWD3), mRNA 
0.41   2  4  4  7  NM_131987.2  CG4202-RA (Sas10), mRNA 
0.41   2  2  23  51  NM_080335.2  CG2984-RA (Pp2C1), mRNA 
0.41   2  2  9  4  NM_141147.2  CG14454-RA (CG14454), mRNA 
0.41   2  2  9  4  NM_168954.1  CG32453-RA (CG32453), mRNA 
0.41   2  2  7  21  NM_139378.2  CG13928-RA (CG13928), mRNA 
0.2   1  2  5  13  NM_130578.2  CG32809-RD, transcript variant D (CG32809), mRNA 
0.2   1  2  2  7  NM_137667.3  CG13442-RA (CG13442), mRNA 
0   0  38  35  35  NM_167524.2  CG9819-RB, transcript variant B (CanA-14F), mRNA 
0   0  38  35  35  NM_167523.2  CG9819-RA, transcript variant A (CanA-14F), mRNA 

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