NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13833R-1

Symbol CG13833 Full Name CG13833

CG No CG13833 Old CG No CG13833

Synonyms CG13833

Accession No (Link to NCBI) NM_142854.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGGTAAAACTGCAGGCCATA

Primer Seq. 3'

aatctagaggtaccTTGGGTAGGAAAACCAGCTTG

Predicted Fragment Size

IR fragment full Seq

0001 CTGGTAAAAC TGCAGGCCAT AATCGCACTG ATCGGTTTGG CGGCCATAAC GcCACTACTC 
0061 ATTTTGGTAG CACTACTCGG CCGACTGATC GCCAAACTCT GCTGGTGCTC GGCGCCGAAA 
0121 AGTATCGCGG GAGAAGTGGC CGTGGTCACT GGAGCTGGTC ATGGACTAGG TCGTGCCATA 
0181 TCCTTGGAAC TGGCTAAAAA AGGCTGTCAC ATCGCCGTGG TGGATATCAA TGTTTCCGGA 
0241 GCGGAAGACA CCGTCAAGCA AATCCAAGAT ATCTATAAGG TTNCGCGCAA AGGCATANAA 
0301 GGCCAATGTA ACCAATTACG ACGATCTAGT TGAGCTGAAC TCCAAAGTGG TCGAGGAAAT 
0361 GGGTCCAGTT ACCGTCCTGG TTAATAATGC TGGTGTCATG ATGCATCGCA ATATGTTTAA 
0421 TCCGGACCCG GCCGATGTGC AGCTAATGAT CAATGTTAAC CTTACGTCGC ATTTCTGGAC 
0481 CAAGCTGGTT TTCCTACCCA A

in silico PCR Fragment

0001 CTGGTAAAAC TGCAGGCCAT AATCGCACTG ATCGGTTTGG CGGCCATAAC GCCACTACTC 
0061 ATTTTGGTAG CACTACTCGG CCGACTGATC GCCAAACTCT GCTGGTGCTC GGCGCCGAAA 
0121 AGTATCGCGG GAGAAGTGGC CGTGGTCACT GGAGCTGGTC ATGGACTAGG TCGTGCCATA 
0181 TCCTTGGAAC TGGCTAAAAA AGGCTGTCAC ATCGCCGTGG TGGATATCAA TGTTTCCGGA 
0241 GCGGAAGACA CCGTCAAGCA AATCCAAGAT ATCTATAAGG TT-CGCGCAA AGGCATACAA 
0301 GGCCAATGTA ACCAATTACG ACGATCTAGT TGAGCTGAAC TCCAAAGTGG TCGAGGAAAT 
0361 GGGTCCAGTT ACCGTCCTGG TTAATAATGC TGGTGTCATG ATGCATCGCA ATATGTTTAA 
0421 TCCGGACCCG GCCGATGTGC AGCTAATGAT CAATGTTAAC CTTACGTCGC ATTTCTGGAC 
0481 CAAGCTGGTT TTCCTACCCA A

Assemble Data


13833R-1.IR_full       1   CTGGTAAAACTGCAGGCCATAATCGCACTGATCGGTTTGGCGGCCATAACGCCACTACTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     1   CTGGTAAAACTGCAGGCCATAATCGCACTGATCGGTTTGGCGGCCATAACGCCACTACTC 60


13833R-1.IR_full       61  ATTTTGGTAGCACTACTCGGCCGACTGATCGCCAAACTCTGCTGGTGCTCGGCGCCGAAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     61  ATTTTGGTAGCACTACTCGGCCGACTGATCGCCAAACTCTGCTGGTGCTCGGCGCCGAAA 120


13833R-1.IR_full       121 AGTATCGCGGGAGAAGTGGCCGTGGTCACTGGAGCTGGTCATGGACTAGGTCGTGCCATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     121 AGTATCGCGGGAGAAGTGGCCGTGGTCACTGGAGCTGGTCATGGACTAGGTCGTGCCATA 180


13833R-1.IR_full       181 TCCTTGGAACTGGCTAAAAAAGGCTGTCACATCGCCGTGGTGGATATCAATGTTTCCGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     181 TCCTTGGAACTGGCTAAAAAAGGCTGTCACATCGCCGTGGTGGATATCAATGTTTCCGGA 240


13833R-1.IR_full       241 GCGGAAGACACCGTCAAGCAAATCCAAGATATCTATAAGGTTNCGCGCAAAGGCATANAA 300
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||| ||
13833R-1.in silico     241 GCGGAAGACACCGTCAAGCAAATCCAAGATATCTATAAGGTT-CGCGCAAAGGCATACAA 300


13833R-1.IR_full       301 GGCCAATGTAACCAATTACGACGATCTAGTTGAGCTGAACTCCAAAGTGGTCGAGGAAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     301 GGCCAATGTAACCAATTACGACGATCTAGTTGAGCTGAACTCCAAAGTGGTCGAGGAAAT 360


13833R-1.IR_full       361 GGGTCCAGTTACCGTCCTGGTTAATAATGCTGGTGTCATGATGCATCGCAATATGTTTAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     361 GGGTCCAGTTACCGTCCTGGTTAATAATGCTGGTGTCATGATGCATCGCAATATGTTTAA 420


13833R-1.IR_full       421 TCCGGACCCGGCCGATGTGCAGCTAATGATCAATGTTAACCTTACGTCGCATTTCTGGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13833R-1.in silico     421 TCCGGACCCGGCCGATGTGCAGCTAATGATCAATGTTAACCTTACGTCGCATTTCTGGAC 480


13833R-1.IR_full       481 CAAGCTGGTTTTCCTACCCAA 501
                           |||||||||||||||||||||
13833R-1.in silico     481 CAAGCTGGTTTTCCTACCCAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142854.1	CG13833-RA (CG13833), mRNA
0	0	2	6	6	NM_166466.2	CG10082-RA, transcript variant A (CG10082), mRNA
0	0	2	6	6	NM_137751.3	CG10082-RB, transcript variant B (CG10082), mRNA
0	0	2	3	2	NM_135207.2	CG11319-RA (CG11319), mRNA
0	0	2	2	2	NM_165059.1	CG7578-RB, transcript variant B (sec71), mRNA
0	0	2	2	2	NM_135831.2	CG7578-RA, transcript variant A (sec71), mRNA
0	0	1	6	4	NM_168297.1	CG32026-RA (CG32026), mRNA
0	0	1	3	7	NM_139980.2	CG6662-RA (CG6662), mRNA
0	0	1	3	2	NM_167442.1	CG9195-RB, transcript variant B (Scamp), mRNA
0	0	1	3	2	NM_132795.2	CG9195-RA, transcript variant A (Scamp), mRNA
0	0	1	2	7	NM_140725.1	CG18265-RA (CG18265), mRNA
0	0	1	2	3	NM_132791.2	CG12608-RA (CG12608), mRNA
0	0	1	2	3	NM_132438.1	CG11203-RA (CG11203), mRNA
0	0	1	2	3	NM_165067.1	CG31811-RB, transcript variant B (cenG1A), mRNA
0	0	1	2	2	NM_078838.2	CG31811-RA, transcript variant A (cenG1A), mRNA
0	0	1	2	2	NM_165068.1	CG31811-RC, transcript variant C (cenG1A), mRNA
0	0	1	2	2	NM_141191.1	CG9766-RA, transcript variant A (CG9766), mRNA
0	0	1	2	2	NM_132790.1	CG9123-RA (CG9123), mRNA
0	0	1	2	2	NM_164326.1	CG9766-RB, transcript variant B (CG9766), mRNA
0	0	1	2	2	NM_136950.2	CG8818-RA (CG8818), mRNA
0	0	0	5	7	NM_205893.1	CG31690-RA, transcript variant A (CG31690), mRNA
0	0	0	5	7	NM_164492.1	CG31690-RB, transcript variant B (CG31690), mRNA
0	0	0	4	6	NM_169865.1	CG6231-RC, transcript variant C (CG6231), mRNA
0	0	0	4	6	NM_165508.1	CG11084-RA, transcript variant A (pk), mRNA
0	0	0	3	8	NM_132017.1	CG3160-RA (CG3160), mRNA
0	0	0	3	8	NM_132056.3	CG4790-RA (fs(1)M3), mRNA
0	0	0	3	4	NM_143432.2	CG31445-RA (CG31445), mRNA
0	0	0	3	4	NM_137977.2	CG11299-RA (CG11299), mRNA
0	0	0	3	3	NM_135199.2	CG31637-RA (CG31637), mRNA
0	0	0	3	3	NM_170250.1	CG31090-RA (CG31090), mRNA

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