NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13760R-2

Symbol CG13760 Full Name CG13760

CG No CG13760 Old CG No CG13760

Synonyms EG:BACR25B3.6, CG13760

Accession No (Link to NCBI) NM_130654.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCACACTGGCCCAAGCTGTTA

Primer Seq. 3'

aatctagaggtaccGGCGTCCTTGAACTTGCGCGT

Predicted Fragment Size

IR fragment full Seq

0001 CCACACTGGC CCAAGCTGTT AGACCAAAAC ATTCCCAGTT CAGTTTCCAA TTGGATGCca 
0061 ggatCAAAGG AGCGCAGCCG CGGGCACAGG AGCACGCGAA GCACACGGAG CCAAGGACTC 
0121 GAGGACCCGA GGAGCACAAG AAGCCAAGAA GAGCCCAGCT GCTGGCCAGA ATGAACGGAC 
0181 TGCCGCCGAG CAAGCACTAC AACCTGACGC ACTACCAGCA GCGCTACAAC TGGGACTGCG 
0241 GTCTGTCCTG CATCATTATG ATCCTGTCGG CGCAGCAAAG GGAACAGCTT CTTGGGAACT 
0301 TTGATGCCGT GTGCGGCGAG GAGGGATTCG GCTCGAGCAC CTGGACCATC GACCTGTGCT 
0361 ATCTGCTGAT GCGCTACCAG GTGCGCCACG AGTACTTCAC CCAAACGCTC GGGATCGATC 
0421 CCAACTACGC GCAGCACACG TACTACTCCA AGATCATCGA CAAGGACGAA CGGCGCGTGA 
0481 CGCGCAAGTT CAAGGACNCC

in silico PCR Fragment

0001 CCACACTGGC CCAAGCTGTT AGACCAAAAC ATTCCCAGTT CAGTTTCCAA TTGGACGCCA 
0061 GGATCAAAGG AGCGCAGCCG CGGGCACAGG AGCACGCGAA GCACACGGAG CCAAGGACTC 
0121 GAGGACCCGA GGAGCACAAG AAGCCAAGAA GAGCCCAGCT GCTGGCCAGA ATGAACGGAC 
0181 TGCCGCCGAG CAAGCACTAC AACCTGACGC ACTACCAGCA GCGCTACAAC TGGGACTGCG 
0241 GTCTGTCCTG CATCATTATG ATCCTGTCGG CGCAGCAAAG GGAACAGCTT CTTGGGAACT 
0301 TTGATGCCGT GTGCGGCGAG GAGGGATTTG GCTCGAGCAC CTGGACCATC GACCTGTGCT 
0361 ATCTGCTGAT GCGCTACCAG GTGCGCCACG AGTACTTCAC CCAAACGCTC GGGATCGATC 
0421 CCAACTACGC GCAGCACACG TACTACTCCA AGATCATCGA CAAGGACGAA CGGCGCGTGA 
0481 CGCGCAAGTT CAAGGACGCC

Assemble Data


13760R-2.IR_full       1   CCACACTGGCCCAAGCTGTTAGACCAAAACATTCCCAGTTCAGTTTCCAATTGGATGCCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
13760R-2.in silico     1   CCACACTGGCCCAAGCTGTTAGACCAAAACATTCCCAGTTCAGTTTCCAATTGGACGCCA 60


13760R-2.IR_full       61  GGATCAAAGGAGCGCAGCCGCGGGCACAGGAGCACGCGAAGCACACGGAGCCAAGGACTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     61  GGATCAAAGGAGCGCAGCCGCGGGCACAGGAGCACGCGAAGCACACGGAGCCAAGGACTC 120


13760R-2.IR_full       121 GAGGACCCGAGGAGCACAAGAAGCCAAGAAGAGCCCAGCTGCTGGCCAGAATGAACGGAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     121 GAGGACCCGAGGAGCACAAGAAGCCAAGAAGAGCCCAGCTGCTGGCCAGAATGAACGGAC 180


13760R-2.IR_full       181 TGCCGCCGAGCAAGCACTACAACCTGACGCACTACCAGCAGCGCTACAACTGGGACTGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     181 TGCCGCCGAGCAAGCACTACAACCTGACGCACTACCAGCAGCGCTACAACTGGGACTGCG 240


13760R-2.IR_full       241 GTCTGTCCTGCATCATTATGATCCTGTCGGCGCAGCAAAGGGAACAGCTTCTTGGGAACT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     241 GTCTGTCCTGCATCATTATGATCCTGTCGGCGCAGCAAAGGGAACAGCTTCTTGGGAACT 300


13760R-2.IR_full       301 TTGATGCCGTGTGCGGCGAGGAGGGATTCGGCTCGAGCACCTGGACCATCGACCTGTGCT 360
                           |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
13760R-2.in silico     301 TTGATGCCGTGTGCGGCGAGGAGGGATTTGGCTCGAGCACCTGGACCATCGACCTGTGCT 360


13760R-2.IR_full       361 ATCTGCTGATGCGCTACCAGGTGCGCCACGAGTACTTCACCCAAACGCTCGGGATCGATC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     361 ATCTGCTGATGCGCTACCAGGTGCGCCACGAGTACTTCACCCAAACGCTCGGGATCGATC 420


13760R-2.IR_full       421 CCAACTACGCGCAGCACACGTACTACTCCAAGATCATCGACAAGGACGAACGGCGCGTGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13760R-2.in silico     421 CCAACTACGCGCAGCACACGTACTACTCCAAGATCATCGACAAGGACGAACGGCGCGTGA 480


13760R-2.IR_full       481 CGCGCAAGTTCAAGGACNCC 500
                           ||||||||||||||||| ||
13760R-2.in silico     481 CGCGCAAGTTCAAGGACGCC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_130654.2	CG13760-RB (CG13760), mRNA
0	0	2	2	5	NM_001014453.1	CG33526-RD, transcript variant D (PNUTS), mRNA
0	0	1	6	3	NM_132518.1	CG9360-RA (CG9360), mRNA
0	0	1	3	3	NM_170548.1	CG1607-RB, transcript variant B (CG1607), mRNA
0	0	1	3	3	NM_143608.1	CG1607-RA, transcript variant A (CG1607), mRNA
0	0	1	3	2	NM_168009.1	CG14959-RB, transcript variant B (CG14959), mRNA
0	0	1	2	6	NM_167307.1	CG4147-RC, transcript variant C (Hsc70-3), mRNA
0	0	1	2	6	NM_167306.1	CG4147-RA, transcript variant A (Hsc70-3), mRNA
0	0	1	2	6	NM_167308.1	CG4147-RD, transcript variant D (Hsc70-3), mRNA
0	0	1	2	6	NM_078577.2	CG4147-RB, transcript variant B (Hsc70-3), mRNA
0	0	1	2	5	NM_078547.2	CG2979-RA (Yp2), mRNA
0	0	1	2	4	NM_134945.1	CG2979-RA (Yp2), mRNA, type IV CG3212-RA (Sr-CIV), mRNA
0	0	1	2	3	NM_079951.2	CG8269-RA (Dmn), mRNA
0	0	1	2	2	NM_140316.2	CG32104-RB (CG32104), mRNA
0	0	1	2	2	NM_078766.2	CG9159-RA (Kr-h2), mRNA
0	0	1	2	2	NM_139945.2	CG13671-RA (CG13671), mRNA
0	0	1	2	2	NM_138229.1	CG13908-RA (CG13908), mRNA
0	0	0	7	2	NM_137345.2	CG15611-RA, transcript variant A (CG15611), mRNA
0	0	0	7	2	NM_166218.1	CG15611-RB, transcript variant B (CG15611), mRNA
0	0	0	5	10	NM_169997.3	CG31163-RB, transcript variant B (CG31163), mRNA
0	0	0	5	10	NM_001043277.1	CG31163-RD, transcript variant D (CG31163), mRNA
0	0	0	5	5	NM_137822.2	CG13506-RA (CG13506), mRNA
0	0	0	5	4	NM_166063.1	CG10145-RA (mspo), mRNA
0	0	0	4	9	NM_138220.1	CG13889-RA (CG13889), mRNA
0	0	0	4	5	NM_057733.3	CG5210-RA (Chit), mRNA
0	0	0	4	5	NM_142640.2	CG16953-RA (CG16953), mRNA
0	0	0	4	4	NM_167529.1	CG32573-RA (CG32573), mRNA
0	0	0	4	3	NM_168375.2	CG32052-RA (CG32052), mRNA
0	0	0	4	3	NM_140495.1	CG12316-RA, transcript variant A (CG12316), mRNA
0	0	0	4	3	NM_135651.2	CG4751-RA (CG4751), mRNA

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