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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13747R-1 
 Symbol CG13747  Full Name CG13747 
 CG No CG13747  Old CG No CG13747 
 Synonyms CG13747 
 Accession No (Link to NCBI) NM_136596.2 
 Inserted Chr. lll 
 Insertional Mutation  2 lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCACCAGCCACACCATCTCATA 
 Primer Seq. 3' 
aatctagaggtaccACAGCTGCTCCATCACGCGAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CACCAGCCAC ACCATCTCAT ACGTGCAATG CCAGAACCTC AAATACAACG TCATTATCCT 
0061 CGGCTGGCTG GGCGTGATCA TCTCCACCGT CATCTTGGGC AGCTCGGCGC TGACCATCCA 
0121 CTTTCGTCCG GACATCGAGC TGCTGCTAAA CCAGTGGCCC ATGAACTTGG TCCCGGTCGC 
0181 AGAACAGCAG CTACTGATCA ATTTGTTGAC CATCTTTAGC TCCATTGTGT ACGGACTGTC 
0241 GCTGATCAAC ATGGGCGTCA GTCTGCTGCT GCTGATTGGT ATTGCCCGGG ACTCAAGCTG 
0301 CCTAATTTAT CCTTGGCTGA TCTATCATGG CGTTATTTTT GGCTTTGGTC TTTACTTGGG 
0361 GGTTTTCTAC GCGACCGCAG GTCTGTTCAT TGACCTGTCG AGCTTTCTAA TGTGTCTTCT 
0421 GGTGTTCAAC CTTGTGCTGG TTATATTTTA TAAGATCTAT CACGAGGTCT TCACCCTATT 
0481 TCGCGTGATG GAGCAGCTGT  
 in silico PCR Fragment 
0001 CACCAGCCAC ACCATCTCAT ACGTGCAATG CCAGAACCTC AAATACAACG TCATTATCCT 
0061 CGGCTGGCTG GGCGTGATCA TCTCCACCGT CATCTTGGGC AGCTCGGCGC TGACCATCCA 
0121 CTTTCGTCCG GACATCGAGC TGCTGCTAAA CCAGTGGCCC ATGAACTTGG TCCCGGTCGC 
0181 AGAACAGCAG CTACTGATCA ATTTGTTGAC CATCTTTAGC TCCATTGTGT ACGGACTGTC 
0241 GCTGATCAAC ATGGGCGTCA GTCTGCTGCT GCTGATTGGT ATTGCCCGGG ACTCAAGCTG 
0301 CCTAATTTAT CCTTGGCTGA TCTATCATGG CGTTATTTTT GGCTTTGGTC TTTACTTGGG 
0361 GGTTTTCTAC GCGACCGCAG GTCTGTTCAT TGACCTGTCG AGCTTTCTAA TGTGTCTTCT 
0421 GGTGTTCAAC CTTGTGCTGG TTATATTTTA TAAGATCTAT CACGAGGTCT TCACCCTATT 
0481 TCGCGTGATG GAGCAGCTGT  
 Assemble Data 

13747R-1.IR_full       1   CACCAGCCACACCATCTCATACGTGCAATGCCAGAACCTCAAATACAACGTCATTATCCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     1   CACCAGCCACACCATCTCATACGTGCAATGCCAGAACCTCAAATACAACGTCATTATCCT 60


13747R-1.IR_full       61  CGGCTGGCTGGGCGTGATCATCTCCACCGTCATCTTGGGCAGCTCGGCGCTGACCATCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     61  CGGCTGGCTGGGCGTGATCATCTCCACCGTCATCTTGGGCAGCTCGGCGCTGACCATCCA 120


13747R-1.IR_full       121 CTTTCGTCCGGACATCGAGCTGCTGCTAAACCAGTGGCCCATGAACTTGGTCCCGGTCGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     121 CTTTCGTCCGGACATCGAGCTGCTGCTAAACCAGTGGCCCATGAACTTGGTCCCGGTCGC 180


13747R-1.IR_full       181 AGAACAGCAGCTACTGATCAATTTGTTGACCATCTTTAGCTCCATTGTGTACGGACTGTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     181 AGAACAGCAGCTACTGATCAATTTGTTGACCATCTTTAGCTCCATTGTGTACGGACTGTC 240


13747R-1.IR_full       241 GCTGATCAACATGGGCGTCAGTCTGCTGCTGCTGATTGGTATTGCCCGGGACTCAAGCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     241 GCTGATCAACATGGGCGTCAGTCTGCTGCTGCTGATTGGTATTGCCCGGGACTCAAGCTG 300


13747R-1.IR_full       301 CCTAATTTATCCTTGGCTGATCTATCATGGCGTTATTTTTGGCTTTGGTCTTTACTTGGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     301 CCTAATTTATCCTTGGCTGATCTATCATGGCGTTATTTTTGGCTTTGGTCTTTACTTGGG 360


13747R-1.IR_full       361 GGTTTTCTACGCGACCGCAGGTCTGTTCATTGACCTGTCGAGCTTTCTAATGTGTCTTCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     361 GGTTTTCTACGCGACCGCAGGTCTGTTCATTGACCTGTCGAGCTTTCTAATGTGTCTTCT 420


13747R-1.IR_full       421 GGTGTTCAACCTTGTGCTGGTTATATTTTATAAGATCTATCACGAGGTCTTCACCCTATT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13747R-1.in silico     421 GGTGTTCAACCTTGTGCTGGTTATATTTTATAAGATCTATCACGAGGTCTTCACCCTATT 480


13747R-1.IR_full       481 TCGCGTGATGGAGCAGCTGT 500
                           ||||||||||||||||||||
13747R-1.in silico     481 TCGCGTGATGGAGCAGCTGT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136596.2  CG13747-RA (CG13747), mRNA 
0.2   1  3  2  6  NM_166055.1  CG10108-RA (phyl), mRNA 
0   0  5  5  4  NM_137139.1  CG12858-RA (CG12858), mRNA 
0   0  5  4  2  NM_170236.1  CG31096-RA (Lgr3), mRNA 
0   0  3  3  4  NM_140158.1  CG6409-RA (CG6409), mRNA 
0   0  2  7  2  NM_001031924.1  CG9130-RB, transcript variant B (CG9130), mRNA 
0   0  2  7  2  NM_001031925.1  CG9130-RA, transcript variant A (CG9130), mRNA 
0   0  2  7  2  NM_001031921.1  CG9133-RD, transcript variant D (CG9133), mRNA 
0   0  2  7  2  NM_001031923.1  CG9133-RC, transcript variant C (CG9133), mRNA 
0   0  2  3  7  NM_169696.1  CG3992-RA, transcript variant A (srp), mRNA 
0   0  2  3  7  NM_169694.1  CG3992-RB, transcript variant B (srp), mRNA 
0   0  2  3  5  NM_001032019.1  CG3992-RD, transcript variant D (srp), mRNA 
0   0  2  3  3  NM_142301.1  CG14889-RA (CG14889), mRNA 
0   0  2  2  2  NM_001038881.1  CG3257-RB, transcript variant B (CG3257), mRNA 
0   0  2  2  2  NM_138043.3  CG3257-RA, transcript variant A (CG3257), mRNA 
0   0  2  2  2  NM_132060.2  CG5941-RA (CG5941), mRNA 
0   0  1  5  11  NM_135077.2  CG14023-RA (CG14023), mRNA 
0   0  1  4  3  NM_057690.2  CG8084-RA (ana), mRNA 
0   0  1  4  2  NM_057365.3  CG3182-RA, transcript variant A (sei), mRNA 
0   0  1  4  2  NM_166653.1  CG3182-RB, transcript variant B (sei), mRNA 
0   0  1  3  7  NM_140184.2  CG7628-RA (CG7628), mRNA 
0   0  1  3  6  NM_206203.1  CG30194-RC, transcript variant C (CG30194), mRNA 
0   0  1  3  2  NM_130624.2  CG16903-RA (CG16903), mRNA 
0   0  1  2  9  NM_168477.1  CG32092-RB (CG32092), mRNA 
0   0  1  2  5  NM_001043068.1  CG8170-RB, transcript variant B (CG8170), mRNA 
0   0  1  2  5  NM_136597.3  CG8170-RA, transcript variant A (CG8170), mRNA 
0   0  1  2  5  NM_169003.2  CG31522-RA, transcript variant A (CG31522), mRNA 
0   0  1  2  5  NM_169002.2  CG31522-RD, transcript variant D (CG31522), mRNA 
0   0  1  2  5  NM_176309.2  CG33205-RC, transcript variant C (CG33205), mRNA 
0   0  1  2  5  NM_176311.2  CG33205-RG, transcript variant G (CG33205), mRNA 

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