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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1372R-2 |
| Symbol |
yl |
Full Name |
yolkless |
| CG No |
CG1372 |
Old CG No |
CG1372 |
| Synonyms |
CT3114, DmYPR, LR13, fs(1)L186, fs(1)yolkless, fs(1)M25, fs(1)A148, fs(1)148, fs(1)K184, fs(1)yl, fs(1)M205, fs(1)L211, fs(1)L196, fs(1)L193, fs(1)5, CG1372, yl, Yl |
| Accession No (Link to NCBI) |
NM_078596.2 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTCAACCTAACCTCGCAGAC |
| Primer Seq. 3' |
aatctagaggtaccGATCCTGCTGGCACATGAACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATTCAACCTA ACCTCGCAGA CACGCGCTCA CCCATCCAGT GGTGGCTCAA CGTCCAGTCG
0061 ATACGGAAAT TGCCAGCGAA CACATCTGAT CATCAATGGA CGCCACGTGG CAATCTCGCT
0121 GCTCCTGCTC GTGGGACTTT GCGGTGGTAC TGCTGCCGGC ACGCCGGGAT CAGCGGACAC
0181 gCGNGTGCGA TGCGGGACAA TTCCAGTGCA GAGACGGCGG CTGCATCTTA CAGGCGAAGA
0241 TGTGCGACGG ACGCGGAGAC TGCAAGGACA GCTCGGATGA ATTGGACTGC GACTACCGAC
0301 TGTGCCGACC GCCACATTGG TTCCCGTGTG CCCAGCCACA CGGAGCCCTG CCTGGCAACG
0361 GAACTAATGT GCAACGGCAT CGACAACTGT CCCGGCGGCG AGGATGAGCT CAACTGCCCG
0421 GTGCGGCCAG GATTCCGATT CGGTGACACC GCTCATCGCA TGCGCAACTG CAGCAAGTAC
0481 GAGTTCATGT GCCAGCAGGA TC |
| in silico PCR Fragment |
0001 ATTCAACCTA ACCTCGCAGA CACGCGCTCA CCCATCCAGT GGTGGCTCAA CGTCCAGTCG
0061 ATACGGAAAT TGCCAGCGAA CACATCTGAT CATCAATGGA CGCCACGTGG CAATCTCGCT
0121 GCTCCTGCTC GTGGGACTTT GCGGTGGTAC TGCTGCCGGC ACGCCGGGAT CAGCGGACAC
0181 GCG-GTGCGA TGCGGGACAA TTCCAGTGCA GAGACGGCGG CTGCATCTTA CAGGCGAAGA
0241 TGTGCGACGG ACGCGGAGAC TGCAAGGACA GCTCGGATGA ATTGGACTGC GACTACCGAC
0301 TGTGCCGACC GCCACATTGG TTCCCGTGTG CCCAGCCACA CGGAG-CCTG CCTGGCAGCG
0361 GAACTAATGT GCAACGGCAT CGACAACTGT CCCGGCGGCG AGGATGAGCT CAACTGCCCG
0421 GTGCGGCCAG GATTCCGATT CGGTGACACC GCTCATCGCA TGCGCAGCTG CAGCAAGTAC
0481 GAGTTCATGT GCCAGCAGGA TC |
| Assemble Data |
1372R-2.IR_full 1 ATTCAACCTAACCTCGCAGACACGCGCTCACCCATCCAGTGGTGGCTCAACGTCCAGTCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 1 ATTCAACCTAACCTCGCAGACACGCGCTCACCCATCCAGTGGTGGCTCAACGTCCAGTCG 60
1372R-2.IR_full 61 ATACGGAAATTGCCAGCGAACACATCTGATCATCAATGGACGCCACGTGGCAATCTCGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 61 ATACGGAAATTGCCAGCGAACACATCTGATCATCAATGGACGCCACGTGGCAATCTCGCT 120
1372R-2.IR_full 121 GCTCCTGCTCGTGGGACTTTGCGGTGGTACTGCTGCCGGCACGCCGGGATCAGCGGACAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 121 GCTCCTGCTCGTGGGACTTTGCGGTGGTACTGCTGCCGGCACGCCGGGATCAGCGGACAC 180
1372R-2.IR_full 181 GCGNGTGCGATGCGGGACAATTCCAGTGCAGAGACGGCGGCTGCATCTTACAGGCGAAGA 240
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 181 GCG-GTGCGATGCGGGACAATTCCAGTGCAGAGACGGCGGCTGCATCTTACAGGCGAAGA 240
1372R-2.IR_full 241 TGTGCGACGGACGCGGAGACTGCAAGGACAGCTCGGATGAATTGGACTGCGACTACCGAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 241 TGTGCGACGGACGCGGAGACTGCAAGGACAGCTCGGATGAATTGGACTGCGACTACCGAC 300
1372R-2.IR_full 301 TGTGCCGACCGCCACATTGGTTCCCGTGTGCCCAGCCACACGGAGCCCTGCCTGGCAACG 360
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| ||
1372R-2.in silico 301 TGTGCCGACCGCCACATTGGTTCCCGTGTGCCCAGCCACACGGAG-CCTGCCTGGCAGCG 360
1372R-2.IR_full 361 GAACTAATGTGCAACGGCATCGACAACTGTCCCGGCGGCGAGGATGAGCTCAACTGCCCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1372R-2.in silico 361 GAACTAATGTGCAACGGCATCGACAACTGTCCCGGCGGCGAGGATGAGCTCAACTGCCCG 420
1372R-2.IR_full 421 GTGCGGCCAGGATTCCGATTCGGTGACACCGCTCATCGCATGCGCAACTGCAGCAAGTAC 480
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
1372R-2.in silico 421 GTGCGGCCAGGATTCCGATTCGGTGACACCGCTCATCGCATGCGCAGCTGCAGCAAGTAC 480
1372R-2.IR_full 481 GAGTTCATGTGCCAGCAGGATC 502
||||||||||||||||||||||
1372R-2.in silico 481 GAGTTCATGTGCCAGCAGGATC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
3 |
8 |
NM_078596.2 |
CG1372-RA, transcript variant A (yl), mRNA |
| 100 |
482 |
0 |
3 |
8 |
NM_206710.1 |
CG1372-RB, transcript variant B (yl), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_140288.2 |
CG6801-RA (l(3)j2D3), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_078805.3 |
CG5838-RA (Dref), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_140957.2 |
CG5528-RA (Toll-9), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_078843.4 |
CG3479-RB, transcript variant B (osp), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_165089.2 |
CG3479-RA, transcript variant A (osp), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142980.2 |
CG5706-RA (CG5706), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_080161.1 |
CG3496-RA (vir), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_078816.2 |
CG7279-RA (Lip1), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141892.2 |
CG14735-RA (CG14735), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165938.1 |
CG8772-RA, transcript variant A (nemy), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_136965.3 |
CG8772-RF, transcript variant F (nemy), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165939.1 |
CG8772-RD, transcript variant D (nemy), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165941.1 |
CG8772-RE, transcript variant E (nemy), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165942.1 |
CG8772-RC, transcript variant C (nemy), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_165943.1 |
CG8772-RB, transcript variant B (nemy), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_164688.1 |
CG31640-RA (CG31640), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_136365.1 |
CG11212-RA (Ptr), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142241.1 |
CG4560-RB, transcript variant B (Arpc3A), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169675.1 |
CG4560-RA, transcript variant A (Arpc3A), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137195.2 |
CG8180-RA (CG8180), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141508.1 |
CG7910-RA (CG7910), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043051.1 |
CG17800-PAP (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043032.1 |
CG17800-PAY (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135305.3 |
CG7149-RA (CG7149), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_136882.2 |
CG30040-RA (jeb), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_134840.2 |
CG9886-RA (CG9886), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_168711.2 |
CG6664-RD, transcript variant D (CG6664), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_140687.4 |
CG6664-RA, transcript variant A (CG6664), mRNA |
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