NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13704R-1

Symbol CG13704 Full Name CG13704

CG No CG13704 Old CG No CG13704

Synonyms BcDNA:RE40159, CG13704

Accession No (Link to NCBI) NM_139682.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Izumi Y, Furuse K, Furuse M.
The novel membrane protein Hoka regulates septate junction organization and stem cell homeostasis in the Drosophila gut.
J Cell Sci (2021) 134(6) [ PubMed ID = 33589496 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGTGCTCCACCTACTTGGTGA

Primer Seq. 3'

aatctagaggtaccGACAATAGCGGTGGCATGCGT

Predicted Fragment Size

IR fragment full Seq

0001 AGTGCTCCAC CTACTTGGTG ATATGCCTGG TACTGTTGGC CTGCTGCCTG CAAGAATCGG 
0061 AGGCCACGCG ACGTGTGAAC CGCGGACGAC GCACCCTTAC CCGCAGATAC TTCACTGGCC 
0121 TTGCTATTCC CGGATGGGCT CTCATCGTTT GTGTAGCGGT AGGTGAGCTG CTGATTGGTG 
0181 GAGCCCTCTA CTTCATCCTC AAGAAGGTGA TCCTGGACAA GGAACCAGAT CANGACGGCG 
0241 GCCTCCTACA CGCCCGCCCA GACTCATGCG ACGGCCACAC CTTATACTCC CGCCCAGACT 
0301 CACGATCCGG CCACGGCCAC AACCTATACT CCTGCTCAGA CTCATGAGAC GGCCAATGTG 
0361 ACTCCCACTC CAACGCATGC CACCGCTATT GTC

in silico PCR Fragment

0001 AGTGCTCCAC CTACTTGGTG ATATGCCTGG TACTGTTGGC CTGCTGCCTG CAAGAATCGG 
0061 AGGCCACGCG ACGTGTGAAC CGCGGACGAC GCACCCTTAC CCGCAGATAC TTCACTGGCC 
0121 TTGCTATTCC CGGATGGGCT CTCATCGTTT GTGTAGCGGT AGGTGAGCTG CTGATTGGTG 
0181 GAGCCCTCTA CTTCATCCTC AAGAAGGTGA TCCTGGACAA GGAACCAGAT CA-GACGGCG 
0241 GCCTCCTACA CGCCCGCCCA GACTCATGCG ACGGCCACAC CTTATACTCC CGCCCAGACT 
0301 CACGATCCGG CCACGGCCAC AACCTATACT CCTGCCCAGA CTCATGAGAC GGCCAATGTG 
0361 ACTCCCACTC CAACGCATGC CACCGCTATT GTC

Assemble Data


13704R-1.IR_full       1   AGTGCTCCACCTACTTGGTGATATGCCTGGTACTGTTGGCCTGCTGCCTGCAAGAATCGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13704R-1.in silico     1   AGTGCTCCACCTACTTGGTGATATGCCTGGTACTGTTGGCCTGCTGCCTGCAAGAATCGG 60


13704R-1.IR_full       61  AGGCCACGCGACGTGTGAACCGCGGACGACGCACCCTTACCCGCAGATACTTCACTGGCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13704R-1.in silico     61  AGGCCACGCGACGTGTGAACCGCGGACGACGCACCCTTACCCGCAGATACTTCACTGGCC 120


13704R-1.IR_full       121 TTGCTATTCCCGGATGGGCTCTCATCGTTTGTGTAGCGGTAGGTGAGCTGCTGATTGGTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13704R-1.in silico     121 TTGCTATTCCCGGATGGGCTCTCATCGTTTGTGTAGCGGTAGGTGAGCTGCTGATTGGTG 180


13704R-1.IR_full       181 GAGCCCTCTACTTCATCCTCAAGAAGGTGATCCTGGACAAGGAACCAGATCANGACGGCG 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
13704R-1.in silico     181 GAGCCCTCTACTTCATCCTCAAGAAGGTGATCCTGGACAAGGAACCAGATCA-GACGGCG 240


13704R-1.IR_full       241 GCCTCCTACACGCCCGCCCAGACTCATGCGACGGCCACACCTTATACTCCCGCCCAGACT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13704R-1.in silico     241 GCCTCCTACACGCCCGCCCAGACTCATGCGACGGCCACACCTTATACTCCCGCCCAGACT 300


13704R-1.IR_full       301 CACGATCCGGCCACGGCCACAACCTATACTCCTGCTCAGACTCATGAGACGGCCAATGTG 360
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
13704R-1.in silico     301 CACGATCCGGCCACGGCCACAACCTATACTCCTGCCCAGACTCATGAGACGGCCAATGTG 360


13704R-1.IR_full       361 ACTCCCACTCCAACGCATGCCACCGCTATTGTC 393
                           |||||||||||||||||||||||||||||||||
13704R-1.in silico     361 ACTCCCACTCCAACGCATGCCACCGCTATTGTC 393

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	374	12	30	56	NM_139682.1	CG13704-RA (CG13704), mRNA
0	0	6	3	3	NM_135203.2	CG11043-RA (CG11043), mRNA
0	0	4	2	6	NM_206442.1	CG1030-RC, transcript variant C (Scr), mRNA
0	0	4	2	6	NM_206443.1	CG1030-RB, transcript variant B (Scr), mRNA
0	0	4	2	6	NM_079524.3	CG1030-RA, transcript variant A (Scr), mRNA
0	0	2	3	3	NM_139776.2	CG10289-RA (CG10289), mRNA
0	0	1	6	5	NM_169955.2	CG10823-RB, transcript variant B (SIFR), mRNA
0	0	1	6	5	NM_142709.2	CG10823-RA, transcript variant A (SIFR), mRNA
0	0	1	4	5	NM_080104.2	CG4032-RA (Abl), mRNA
0	0	1	4	4	NM_139929.3	CG7387-RA (CG7387), mRNA
0	0	1	3	2	NM_164576.2	CG15427-RD, transcript variant D (tutl), mRNA
0	0	1	3	2	NM_170110.2	CG5405-RC, transcript variant C (KrT95D), mRNA
0	0	1	3	2	NM_170108.2	CG5405-RB, transcript variant B (KrT95D), mRNA
0	0	1	3	2	NM_079749.4	CG5405-RA, transcript variant A (KrT95D), mRNA
0	0	1	2	5	NM_135236.2	CG10806-RB, transcript variant B (CG10806), mRNA
0	0	1	2	5	NM_164717.1	CG10806-RA, transcript variant A (CG10806), mRNA
0	0	1	2	3	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0	0	1	2	2	NM_078537.2	CG10966-RA, transcript variant A (rdgA), mRNA
0	0	1	2	2	NM_001042799.1	CG10966-RB, transcript variant B (rdgA), mRNA
0	0	1	2	2	NM_170030.1	CG5248-RD, transcript variant D (loco), mRNA
0	0	0	6	3	NM_057825.3	CG2928-RA (Reg-5), mRNA
0	0	0	5	8	NM_166696.1	CG9071-RB, transcript variant B (NaCP60E), mRNA
0	0	0	5	3	NM_206412.1	CG10508-RF, transcript variant F (CG10508), mRNA
0	0	0	5	2	NM_176384.2	CG10508-RC, transcript variant C (CG10508), mRNA
0	0	0	4	8	NM_140521.1	CG6498-RA (CG6498), mRNA
0	0	0	4	3	NM_165635.1	CG8251-RB, transcript variant B (Pgi), mRNA
0	0	0	4	3	NM_165636.1	CG8251-RC, transcript variant C (Pgi), mRNA
0	0	0	4	3	NM_078939.2	CG8251-RA, transcript variant A (Pgi), mRNA
0	0	0	4	2	NM_141907.2	CG10014-RA (CG10014), mRNA
0	0	0	3	8	NM_080047.2	CG8491-RA (kto), mRNA

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