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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13688R-2 
 Symbol Ipk2  Full Name Ipk2 
 CG No CG13688  Old CG No CG13688 
 Synonyms CG13688, dmIpk2, Ipk2 
 Accession No (Link to NCBI) NM_134691.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGGAGCTTCCCGAGGGATTT 
 Primer Seq. 3' 
aatctagaggtaccCGCAGGATTGTGGTCTCCTGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 cAGGNAGCTT CCCGAGGGAT TTCGCCAGCT AAAGACACAG GTTGCGGGTC ACACATTCGA 
0061 GGAAAGCAAC GCGGAGBCAG TGGGTCTTCT GCAGGACTCG AAGGCGGGAT GCGTACTGAA 
0121 GCCCCTGGGG AAGCCGGAGT GCGGCGANAC GGGAGCTGCG CTTCTATGAG TCACTCGCAG 
0181 AGGCAGGTGC TTCAGGTGAT AATGACCTCC TGGCGCTGCT CCGCGGTCAC GTCCCCCGGT 
0241 TTTACGGCCC CCTAAAACTG GTTGTGAATC GGCGCGAGCG CACGTTCCTG CGCCTGGAAG 
0301 ACCTCACGCG ATCGTACGCA AANGCNCGTG CGTNCATGGA CGTGNAAGAT GGGTAAACGA 
0361 ACCTGGGATC CAGAGTCCTC GCCCAACAAA CGAAAGGTTG AGGAGGCCAA GTACGTTATG 
0421 TGCAAGCAAA AGCTGGGCCT TTGCNTTGCC GGNGATTCCA AGTGTACCTT CCCAAGGAGG 
0481 AGCACACTCA GGAGACCACA ATCCTGCG 
 in silico PCR Fragment 
0001 CAGG-AGCTT CCCGAGGGAT TTCGCCAGCT AAAGACACAG GTTGCGGGTC ACACATTCGA 
0061 GGAAAGCAAC GCGGAGGCAG TGGGTCTTCT GCAGGACTCG AAGGCGGGAT GCGTACTGAA 
0121 GCCTCTGGGG AAGCCGGAGT GCGGCGA-AC GGGAGCTGCG CTTCTATGAG TCACTCGCAG 
0181 AGGCAGGTGC TTCAGGTGAT AATGACCTCC TGGCGCTGCT CCGCGGTCAC GTCCCCCGGT 
0241 TTTACGGCCC CCTAAAACTG GTTGTGAATC GGCGCGAGCG CACGTTCCTG CGCCTGGAAG 
0301 ACCTCACGCG ATCGTACGCA AA-GC-CGTG CGT-CATGGA CGTG-AAGAT GGGTAAACGA 
0361 ACTTGGGATC CAGAGTCCTC GCCCAACAAA CGAAAGGTTG AGGAGGCCAA GTACGTTATG 
0421 TGCAAGCAAA AGCTGGGCCT TTGC-TTGCC GG-GATTCCA AGTGTACCTT CCCAAGGAGG 
0481 AGCACACTCA GGAGACCACA ATCCTGCG 
 Assemble Data 

13688R-2.IR_full       1   CAGGNAGCTTCCCGAGGGATTTCGCCAGCTAAAGACACAGGTTGCGGGTCACACATTCGA 60
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
13688R-2.in silico     1   CAGG-AGCTTCCCGAGGGATTTCGCCAGCTAAAGACACAGGTTGCGGGTCACACATTCGA 60


13688R-2.IR_full       61  GGAAAGCAACGCGGAGBCAGTGGGTCTTCTGCAGGACTCGAAGGCGGGATGCGTACTGAA 120
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
13688R-2.in silico     61  GGAAAGCAACGCGGAGGCAGTGGGTCTTCTGCAGGACTCGAAGGCGGGATGCGTACTGAA 120


13688R-2.IR_full       121 GCCCCTGGGGAAGCCGGAGTGCGGCGANACGGGAGCTGCGCTTCTATGAGTCACTCGCAG 180
                           ||| ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
13688R-2.in silico     121 GCCTCTGGGGAAGCCGGAGTGCGGCGA-ACGGGAGCTGCGCTTCTATGAGTCACTCGCAG 180


13688R-2.IR_full       181 AGGCAGGTGCTTCAGGTGATAATGACCTCCTGGCGCTGCTCCGCGGTCACGTCCCCCGGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13688R-2.in silico     181 AGGCAGGTGCTTCAGGTGATAATGACCTCCTGGCGCTGCTCCGCGGTCACGTCCCCCGGT 240


13688R-2.IR_full       241 TTTACGGCCCCCTAAAACTGGTTGTGAATCGGCGCGAGCGCACGTTCCTGCGCCTGGAAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13688R-2.in silico     241 TTTACGGCCCCCTAAAACTGGTTGTGAATCGGCGCGAGCGCACGTTCCTGCGCCTGGAAG 300


13688R-2.IR_full       301 ACCTCACGCGATCGTACGCAAANGCNCGTGCGTNCATGGACGTGNAAGATGGGTAAACGA 360
                           |||||||||||||||||||||| || ||||||| |||||||||| |||||||||||||||
13688R-2.in silico     301 ACCTCACGCGATCGTACGCAAA-GC-CGTGCGT-CATGGACGTG-AAGATGGGTAAACGA 360


13688R-2.IR_full       361 ACCTGGGATCCAGAGTCCTCGCCCAACAAACGAAAGGTTGAGGAGGCCAAGTACGTTATG 420
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13688R-2.in silico     361 ACTTGGGATCCAGAGTCCTCGCCCAACAAACGAAAGGTTGAGGAGGCCAAGTACGTTATG 420


13688R-2.IR_full       421 TGCAAGCAAAAGCTGGGCCTTTGCNTTGCCGGNGATTCCAAGTGTACCTTCCCAAGGAGG 480
                           |||||||||||||||||||||||| ||||||| |||||||||||||||||||||||||||
13688R-2.in silico     421 TGCAAGCAAAAGCTGGGCCTTTGC-TTGCCGG-GATTCCAAGTGTACCTTCCCAAGGAGG 480


13688R-2.IR_full       481 AGCACACTCAGGAGACCACAATCCTGCG 508
                           ||||||||||||||||||||||||||||
13688R-2.in silico     481 AGCACACTCAGGAGACCACAATCCTGCG 508



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134691.1  CG13688-RA (Ipk2), mRNA 
0   0  2  4  3  NM_137623.2  CG9143-RA (CG9143), mRNA 
0   0  2  3  3  NM_165538.1  CG1600-RA, transcript variant A (CG1600), mRNA 
0   0  2  3  3  NM_136449.2  CG1600-RC, transcript variant C (CG1600), mRNA 
0   0  2  3  3  NM_165539.1  CG1600-RB, transcript variant B (CG1600), mRNA 
0   0  2  2  2  NM_176711.2  CG1543-RB (Tbh), mRNA 
0   0  1  4  2  NM_079395.2  CG9695-RA (Dab), mRNA 
0   0  1  3  6  NM_001043113.1  CG33484-RC, transcript variant C (zormin), mRNA 
0   0  1  3  6  NM_206240.1  CG33484-RA, transcript variant A (zormin), mRNA 
0   0  1  3  6  NM_001043112.1  CG33484-RB, transcript variant B (zormin), mRNA 
0   0  1  3  6  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  1  3  2  NM_165065.1  CG31842-RA (mRpS23), mRNA 
0   0  1  2  6  NM_137632.3  CG8929-RA, transcript variant A (CG8929), mRNA 
0   0  1  2  6  NM_166389.1  CG8929-RB, transcript variant B (CG8929), mRNA 
0   0  1  2  6  NM_166390.1  CG8929-RC, transcript variant C (CG8929), mRNA 
0   0  1  2  5  NM_132516.2  CG2457-RA (inaF), mRNA 
0   0  1  2  4  NM_132059.2  CG4766-RA (CG4766), mRNA 
0   0  1  2  2  NM_167649.1  CG32533-RA (CG32533), mRNA 
0   0  0  6  2  NM_166122.2  CG8295-RC, transcript variant C (Mlf), mRNA 
0   0  0  6  2  NM_079029.3  CG8295-RA, transcript variant A (Mlf), mRNA 
0   0  0  6  2  NM_166123.3  CG8295-RB, transcript variant B (Mlf), mRNA 
0   0  0  6  2  NM_206125.1  CG8295-RD, transcript variant D (Mlf), mRNA 
0   0  0  4  5  NM_058082.2  CG6193-RA (Apc2), mRNA 
0   0  0  4  3  NM_141394.2  CG10284-RA (CG10284), mRNA 
0   0  0  4  3  NM_078851.2  CG4192-RA (kek3), mRNA 
0   0  0  4  2  NM_167302.1  CG1841-RB, transcript variant B (Tango10), mRNA 
0   0  0  4  2  NM_132515.2  CG1841-RA, transcript variant A (Tango10), mRNA 
0   0  0  4  2  NM_164842.1  CG9552-RA (rost), mRNA 
0   0  0  4  2  NM_135342.2  CG8506-RA (CG8506), mRNA 
0   0  0  3  11  NM_170420.1  CG18741-RA, transcript variant A (DopR2), mRNA 

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