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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13603R-3 
 Symbol CG13603  Full Name CG13603 
 CG No CG13603  Old CG No CG13603 
 Synonyms CG13603 
 Accession No (Link to NCBI) NM_142943.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGCTTTGGCCAACGTGCTG 
 Primer Seq. 3' 
aatctagaggtaccAATTTTTTCCGTGTCGCCGTG 
 Predicted Fragment Size 
459 
 IR fragment full Seq 
0001 ATGGCTTTGG CCAACGTGCT GCAGCTAAGC CAGATAGCTG GCCATGTCAT CCTGTTTATC 
0061 CTGTCGCTGT GCATCGTCGT GCCATTGTTT ATAAACCTCG ACCAATTCTG TGGCCACTGC 
0121 CTGCTCTTTA CAACTGGCAA ATAGCGGGAG GAGGACGGCA TGTTCGATGT CCAGTGGGCT 
0181 TCCAATGGCT TCTGCAACTT TCCCCTGATT ACCGGGTTCT TCCTGCTAAT TATTTCGGTG 
0241 GTGCAGATTT ATAGATACGC CCGCATGAAG GAGGAGGCCT CGTTTATAGC TCTGTTCATT 
0301 GACGTCGTTC TGGGCATTTG GATGCTGGCT ATGTCCATTT TGTCGGCTAT TTTCATTACC 
0361 CTGGGCTTTA TTGTCTGGTG CGATTCCATG ACCGAGAGAT TCCCATCCTG TGCCATTTCC 
0421 GCCGGTCAGA ACATTATCCA CGGCGACACG GAAAAAATT 
 in silico PCR Fragment 
0001 ATGGCTTTGG CCAACGTGCT GCAGCTAAGC CAGATAGCTG GCCATGTCAT CCTGTTTATC 
0061 CTGTCGCTGT GCATCGTCGT GCCATTGTTT ATAAACCTCG ACCAATTCTG TGGCCACTGC 
0121 CTGCTCTTTA CAACTGGCAA ATGGCGGGAG GAGGACGGCA TGTTCGATGT CCAGTGGGCT 
0181 TCCAATGGCT TCTGCAACTT TCCCCTGATT ACCGGGTTCT TCCTGCTAAT TATTTCGGTG 
0241 GTGCAGATTT ATAGATACGC CCGCATGAAG GAGGAGGCCT CGTTTATAGC TCTGTTCATT 
0301 GACGTCGTTC TGGGCATTTG GATGCTGGCT ATGTCCATTT TGTCGGCTAT TTTCATTACC 
0361 CTGGGCTTTA TTGTCTGGTG CGATTCCATG ACCGAGAGAT TCCCATCCTG TGCCATTTCC 
0421 GCCGGTCAGA ACATTATCCA CGGCGACACG GAAAAAATT 
 Assemble Data 

13603R-3.IR_full       1   ATGGCTTTGGCCAACGTGCTGCAGCTAAGCCAGATAGCTGGCCATGTCATCCTGTTTATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     1   ATGGCTTTGGCCAACGTGCTGCAGCTAAGCCAGATAGCTGGCCATGTCATCCTGTTTATC 60


13603R-3.IR_full       61  CTGTCGCTGTGCATCGTCGTGCCATTGTTTATAAACCTCGACCAATTCTGTGGCCACTGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     61  CTGTCGCTGTGCATCGTCGTGCCATTGTTTATAAACCTCGACCAATTCTGTGGCCACTGC 120


13603R-3.IR_full       121 CTGCTCTTTACAACTGGCAAATAGCGGGAGGAGGACGGCATGTTCGATGTCCAGTGGGCT 180
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
13603R-3.in silico     121 CTGCTCTTTACAACTGGCAAATGGCGGGAGGAGGACGGCATGTTCGATGTCCAGTGGGCT 180


13603R-3.IR_full       181 TCCAATGGCTTCTGCAACTTTCCCCTGATTACCGGGTTCTTCCTGCTAATTATTTCGGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     181 TCCAATGGCTTCTGCAACTTTCCCCTGATTACCGGGTTCTTCCTGCTAATTATTTCGGTG 240


13603R-3.IR_full       241 GTGCAGATTTATAGATACGCCCGCATGAAGGAGGAGGCCTCGTTTATAGCTCTGTTCATT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     241 GTGCAGATTTATAGATACGCCCGCATGAAGGAGGAGGCCTCGTTTATAGCTCTGTTCATT 300


13603R-3.IR_full       301 GACGTCGTTCTGGGCATTTGGATGCTGGCTATGTCCATTTTGTCGGCTATTTTCATTACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     301 GACGTCGTTCTGGGCATTTGGATGCTGGCTATGTCCATTTTGTCGGCTATTTTCATTACC 360


13603R-3.IR_full       361 CTGGGCTTTATTGTCTGGTGCGATTCCATGACCGAGAGATTCCCATCCTGTGCCATTTCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     361 CTGGGCTTTATTGTCTGGTGCGATTCCATGACCGAGAGATTCCCATCCTGTGCCATTTCC 420


13603R-3.IR_full       421 GCCGGTCAGAACATTATCCACGGCGACACGGAAAAAATT 459
                           |||||||||||||||||||||||||||||||||||||||
13603R-3.in silico     421 GCCGGTCAGAACATTATCCACGGCGACACGGAAAAAATT 459



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   441  0  0  0  NM_142943.2  CG13603-RA (CG13603), mRNA 
0   0  3  2  4  NM_141114.2  CG7143-RA (DNApol-eta), mRNA 
0   0  2  5  4  NM_169974.1  CG31335-RA (Gr93d), mRNA 
0   0  2  5  3  NM_166531.1  CG6044-RB, transcript variant B (CG6044), mRNA 
0   0  2  5  3  NM_206201.1  CG6044-RC, transcript variant C (CG6044), mRNA 
0   0  2  5  3  NM_137824.1  CG6044-RA, transcript variant A (CG6044), mRNA 
0   0  2  2  4  NM_168364.1  CG16707-RB, transcript variant B (vsg), mRNA 
0   0  2  2  4  NM_140092.1  CG16707-RC, transcript variant C (vsg), mRNA 
0   0  2  2  4  NM_168363.1  CG16707-RA, transcript variant A (vsg), mRNA 
0   0  2  2  4  NM_168362.1  CG16707-RD, transcript variant D (vsg), mRNA 
0   0  2  2  2  NM_168903.2  CG5683-RA, transcript variant A (Aef1), mRNA 
0   0  2  2  2  NM_168904.2  CG5683-RC, transcript variant C (Aef1), mRNA 
0   0  2  2  2  NM_079476.2  CG5683-RB, transcript variant B (Aef1), mRNA 
0   0  1  5  5  NM_078536.3  CG12154-RA, transcript variant A (oc), mRNA 
0   0  1  5  5  NM_001014727.1  CG12154-RB, transcript variant B (oc), mRNA 
0   0  1  4  5  NM_165148.1  CG31819-RA (CG31819), mRNA 
0   0  1  3  3  NM_136593.1  CG8181-RA (CG8181), mRNA 
0   0  1  3  2  NM_143715.2  CG8844-RA, transcript variant A (Pdsw), mRNA 
0   0  1  3  2  NM_164537.1  CG8844-RB, transcript variant B (Pdsw), mRNA 
0   0  1  2  3  NM_057292.3  CG5596-RB, transcript variant B (Mlc1), mRNA 
0   0  1  2  3  NM_057291.3  CG5596-RA, transcript variant A (Mlc1), mRNA 
0   0  1  2  2  NM_136409.1  CG17002-RB (CG17002), mRNA 
0   0  0  5  6  NM_140317.2  CG14122-RA (CG14122), mRNA 
0   0  0  5  3  NM_079614.2  CG7996-RA (snk), mRNA 
0   0  0  5  2  NM_133139.1  CG14196-RA (CG14196), mRNA 
0   0  0  4  6  NM_169137.1  CG10295-RC, transcript variant C (Pak), mRNA 
0   0  0  4  6  NM_169136.1  CG10295-RA, transcript variant A (Pak), mRNA 
0   0  0  4  6  NM_079942.3  CG10295-RB, transcript variant B (Pak), mRNA 
0   0  0  4  4  NM_132143.1  CG4586-RA (CG4586), mRNA 
0   0  0  4  4  NM_080421.3  CG18730-RA (Amy-p), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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