National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13597R-4 
 Symbol CG13597  Full Name CG13597 
 CG No CG13597  Old CG No CG13597 
 Synonyms CG13597 
 Accession No (Link to NCBI) NM_142933.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCGCGCAATGAGCCGTATCTG 
 Primer Seq. 3' 
aatctagaggtaccGCTCGAGGTGCTGAGAACGAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCGCGCAATG AGCCGTATCT GCAGCCGGTG AATGGCATCG TGCAACCGCC GGTGATTCCG 
0061 CCACCGAATC GTCCTGGGCG AAGGACAAAC ATCCTAGAAG AGCTCAAGTC CGTGCTGAAT 
0121 TGCTTATGGC GCAATCGCTT CTCGTATCAC TTTCGCCATC CCGTCGATTC TGTGAGCCTG 
0181 GGTGTGCCCG ACTACCATGC GGTGGTCAAG CATCCGATGG ACTTGAGTAC AATTAGGAAG 
0241 CGACTGCACA ACAAATACTA CTGGCAGGCG AGCGAGGCTC TGGAGGATTT CAAGCTGATA 
0301 TTCGATAACT GCTTGTTGTA CAATTTGGAG GGCTCACCGG TTTATCAGGC AGGGAAGCTT 
0361 CTAATGGAGG CCTTTTACAT GCGAATGGAG TCCATTGATT TGAGCACGGA GGTGGAACTA 
0421 AAGCCGAAGT CGGAAAAGCG CAAGAGGAAA GGCTACTGAA TCATTGGATC AAGCCTCGAC 
0481 TTCGTTCTCA GCACCTCGAG C 
 in silico PCR Fragment 
0001 CCGCGCAATG AGCCGTATCT GCAGCCGGTG AATGGCATCG TGCAACCGCC GGTGATTCCG 
0061 CCACCGAATC GTCCTGGGCG AAGGACAAAC ATCCTAGAAG AGCTCAAGTC CGTGCTGAAT 
0121 TGCTTATGGC GCAATCGCTT CTCGTATCAC TTTCGCCATC CCGTCGATTC TGTGAGCCTG 
0181 GGTGTGCCCG ACTACCATGC GGTGGTCAAG CATCCGATGG ACTTGAGTAC AATTAGGAAG 
0241 CGACTGCACA ACAAATACTA CTGGCAGGCG AGCGAGGCTC TGGAGGATTT CAAGCTGATA 
0301 TTCGATAACT GCTTGTTGTA CAATTTGGAG GGCTCACCGG TTTATCAGGC AGGGAAGCTT 
0361 CTAATGGAGG CCTTTTACAT GCGAATGGAG TCCATTGATT TGAGCACGGA GGTGGAACTA 
0421 AAGCCGAAGT CGGAAAAGCG CAAGA-GAAA GGCTACTGAA TCATTGGATC AAGCCTCGAC 
0481 TTCGTTCTCA GCACCTCGAG C 
 Assemble Data 

13597R-4.IR_full       1   CCGCGCAATGAGCCGTATCTGCAGCCGGTGAATGGCATCGTGCAACCGCCGGTGATTCCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     1   CCGCGCAATGAGCCGTATCTGCAGCCGGTGAATGGCATCGTGCAACCGCCGGTGATTCCG 60


13597R-4.IR_full       61  CCACCGAATCGTCCTGGGCGAAGGACAAACATCCTAGAAGAGCTCAAGTCCGTGCTGAAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     61  CCACCGAATCGTCCTGGGCGAAGGACAAACATCCTAGAAGAGCTCAAGTCCGTGCTGAAT 120


13597R-4.IR_full       121 TGCTTATGGCGCAATCGCTTCTCGTATCACTTTCGCCATCCCGTCGATTCTGTGAGCCTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     121 TGCTTATGGCGCAATCGCTTCTCGTATCACTTTCGCCATCCCGTCGATTCTGTGAGCCTG 180


13597R-4.IR_full       181 GGTGTGCCCGACTACCATGCGGTGGTCAAGCATCCGATGGACTTGAGTACAATTAGGAAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     181 GGTGTGCCCGACTACCATGCGGTGGTCAAGCATCCGATGGACTTGAGTACAATTAGGAAG 240


13597R-4.IR_full       241 CGACTGCACAACAAATACTACTGGCAGGCGAGCGAGGCTCTGGAGGATTTCAAGCTGATA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     241 CGACTGCACAACAAATACTACTGGCAGGCGAGCGAGGCTCTGGAGGATTTCAAGCTGATA 300


13597R-4.IR_full       301 TTCGATAACTGCTTGTTGTACAATTTGGAGGGCTCACCGGTTTATCAGGCAGGGAAGCTT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     301 TTCGATAACTGCTTGTTGTACAATTTGGAGGGCTCACCGGTTTATCAGGCAGGGAAGCTT 360


13597R-4.IR_full       361 CTAATGGAGGCCTTTTACATGCGAATGGAGTCCATTGATTTGAGCACGGAGGTGGAACTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13597R-4.in silico     361 CTAATGGAGGCCTTTTACATGCGAATGGAGTCCATTGATTTGAGCACGGAGGTGGAACTA 420


13597R-4.IR_full       421 AAGCCGAAGTCGGAAAAGCGCAAGAGGAAAGGCTACTGAATCATTGGATCAAGCCTCGAC 480
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
13597R-4.in silico     421 AAGCCGAAGTCGGAAAAGCGCAAGA-GAAAGGCTACTGAATCATTGGATCAAGCCTCGAC 480


13597R-4.IR_full       481 TTCGTTCTCAGCACCTCGAGC 501
                           |||||||||||||||||||||
13597R-4.in silico     481 TTCGTTCTCAGCACCTCGAGC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_142933.1  CG13597-RA (CG13597), mRNA 
0   0  2  2  4  NM_001043086.1  CG30084-RG, transcript variant G (CG30084), mRNA 
0   0  2  2  4  NM_137214.3  CG30084-RA, transcript variant A (CG30084), mRNA 
0   0  2  2  4  NM_001032249.1  CG30084-RF, transcript variant F (CG30084), mRNA 
0   0  2  2  4  NM_001032250.1  CG30084-RE, transcript variant E (CG30084), mRNA 
0   0  2  2  4  NM_145757.2  CG30084-RC, transcript variant C (CG30084), mRNA 
0   0  1  8  2  NM_080358.2  CG1484-RA (fliI), mRNA 
0   0  1  3  2  NM_132933.2  CG9609-RA (CG9609), mRNA 
0   0  1  2  2  NM_134878.1  CG3117-RA (CG3117), mRNA 
0   0  1  2  2  NM_137117.1  CG10104-RA (CG10104), mRNA 
0   0  1  2  2  NM_132134.2  CG4558-RA (CG4558), mRNA 
0   0  1  2  2  NM_137951.1  CG5357-RA (CG5357), mRNA 
0   0  0  7  3  NM_058112.3  CG13388-RA, transcript variant A (Akap200), mRNA 
0   0  0  7  3  NM_175984.1  CG13388-RD, transcript variant D (Akap200), mRNA 
0   0  0  5  6  NM_140404.1  CG10743-RA, transcript variant A (CG10743), mRNA 
0   0  0  5  6  NM_168556.1  CG10743-RB, transcript variant B (CG10743), mRNA 
0   0  0  5  4  NM_166665.1  CG3376-RB, transcript variant B (CG3376), mRNA 
0   0  0  5  4  NM_138060.2  CG3376-RA, transcript variant A (CG3376), mRNA 
0   0  0  4  5  NM_001014469.1  CG14039-RF, transcript variant F (qtc), mRNA 
0   0  0  4  5  NM_001014467.1  CG14039-RE, transcript variant E (qtc), mRNA 
0   0  0  4  5  NM_078756.3  CG14039-RA, transcript variant A (qtc), mRNA 
0   0  0  4  5  NM_164626.1  CG14039-RD, transcript variant D (qtc), mRNA 
0   0  0  4  5  NM_164627.1  CG14039-RC, transcript variant C (qtc), mRNA 
0   0  0  4  5  NM_001014468.1  CG14039-RG, transcript variant G (qtc), mRNA 
0   0  0  4  5  NM_164625.1  CG14039-RB, transcript variant B (qtc), mRNA 
0   0  0  4  4  NM_137374.2  CG30104-RA, transcript variant A (CG30104), mRNA 
0   0  0  4  4  NM_166232.1  CG30104-RB, transcript variant B (CG30104), mRNA 
0   0  0  4  4  NM_143168.1  CG17197-RA (CG17197), mRNA 
0   0  0  4  4  NM_165511.2  CG30385-RA (CG30385), mRNA 
0   0  0  4  3  NM_168369.1  CG8177-RG, transcript variant G (CG8177), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.