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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13549R-2 
 Symbol yip3  Full Name yippee interacting protein 3 
 CG No CG13549  Old CG No CG13549 
 Synonyms bs18b12.y1, NEST:bs18b12, CG13549, BcDNA:AT20887, yip3 
 Accession No (Link to NCBI) NM_166588.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGATTCAAGGCAACATCGTGG 
 Primer Seq. 3' 
aatctagaggtaccGGCACCTTGTTCCTCGATTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGATTCAAGG CAACATCGTG GGTCTTAAGA GTACCAACGC CGTGATACTG GCCACCGACT 
0061 CGAAGGAATA CGAAATGTAT CTAATAGATG ACCGCATTTA CTGCTGCGCA CCTCGTTCCG 
0121 GCATCGATCG GAACATAGTT CTGGAAGTGA GTTCAAAGGT GGCAAATCTA GTCCGGGATC 
0181 GCGGCCAGAA TGTGACCGTG TCCCAGGTGC GGGACATGTT TTGCGAAAAG TACCAGACCG 
0241 CCGAATCCCC AAATGTCATG ATAGCTGGCC AGGACAGCAG GGGCCTTCAC TTATTCAGCA 
0301 TGGAGTGTGG AAAGTCACGC ATGGTGATGT ATGGTGCAAA GGGCAGAGAT AAGGAGAACA 
0361 TTGTGGACTT TCTCTCGAAA GACTGGAATG ATTTCATAAN ACCTCAGCGA GGCTGAACAG 
0421 TTGGCCAGGA AAGCGCTTAG GTGGGAGCAC GTGGAAATGT GCACCATCTA TAGGGCTAAA 
0481 GAAATCGAGG AACAAGGTGC C 
 in silico PCR Fragment 
0001 CGATTCAAGG CAACATCGTG GGTCTTAAGA GTACCAACGC CGTGATACTG GCCACCGACT 
0061 CGAAGGAATA CGAAATGTAT CTAATAGATG ACCGCATTTA CTGCTGCGCA CCTCGTTCCG 
0121 GCATCGATCG GAACATAGTT CTGGAAGTGA GTTCAAAGGT GGCAAATCTA GTCCGGGATC 
0181 GCGGCCAGAA TGTGACCGTG TCCCAGGTGC GGGACATGTT TTGCGAAAAG TACCAGACCG 
0241 CCGAATCCCC AAATGTCATG ATAGCTGGCC AGGACAGCAG GGGCCTTCAC TTATTCAGCA 
0301 TGGAGTGTGG AAAGTCACGC ATGGTGATGT ATGGTGCAAA GGGCAGAGAT AAGGAGAACA 
0361 TTGTGGACTT TCTCTCGAAA GACTGGAATG ATTTCATAA- ACCTCAGCGA GGCTGAACAG 
0421 TTGGCCAGGA AAGCGCTTAG GTGGGAGCAC GTGGAAATGT GCACCATCTA TAGGGCTAAA 
0481 GAAATCGAGG AACAAGGTGC C 
 Assemble Data 

13549R-2.IR_full       1   CGATTCAAGGCAACATCGTGGGTCTTAAGAGTACCAACGCCGTGATACTGGCCACCGACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     1   CGATTCAAGGCAACATCGTGGGTCTTAAGAGTACCAACGCCGTGATACTGGCCACCGACT 60


13549R-2.IR_full       61  CGAAGGAATACGAAATGTATCTAATAGATGACCGCATTTACTGCTGCGCACCTCGTTCCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     61  CGAAGGAATACGAAATGTATCTAATAGATGACCGCATTTACTGCTGCGCACCTCGTTCCG 120


13549R-2.IR_full       121 GCATCGATCGGAACATAGTTCTGGAAGTGAGTTCAAAGGTGGCAAATCTAGTCCGGGATC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     121 GCATCGATCGGAACATAGTTCTGGAAGTGAGTTCAAAGGTGGCAAATCTAGTCCGGGATC 180


13549R-2.IR_full       181 GCGGCCAGAATGTGACCGTGTCCCAGGTGCGGGACATGTTTTGCGAAAAGTACCAGACCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     181 GCGGCCAGAATGTGACCGTGTCCCAGGTGCGGGACATGTTTTGCGAAAAGTACCAGACCG 240


13549R-2.IR_full       241 CCGAATCCCCAAATGTCATGATAGCTGGCCAGGACAGCAGGGGCCTTCACTTATTCAGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     241 CCGAATCCCCAAATGTCATGATAGCTGGCCAGGACAGCAGGGGCCTTCACTTATTCAGCA 300


13549R-2.IR_full       301 TGGAGTGTGGAAAGTCACGCATGGTGATGTATGGTGCAAAGGGCAGAGATAAGGAGAACA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     301 TGGAGTGTGGAAAGTCACGCATGGTGATGTATGGTGCAAAGGGCAGAGATAAGGAGAACA 360


13549R-2.IR_full       361 TTGTGGACTTTCTCTCGAAAGACTGGAATGATTTCATAANACCTCAGCGAGGCTGAACAG 420
                           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
13549R-2.in silico     361 TTGTGGACTTTCTCTCGAAAGACTGGAATGATTTCATAA-ACCTCAGCGAGGCTGAACAG 420


13549R-2.IR_full       421 TTGGCCAGGAAAGCGCTTAGGTGGGAGCACGTGGAAATGTGCACCATCTATAGGGCTAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13549R-2.in silico     421 TTGGCCAGGAAAGCGCTTAGGTGGGAGCACGTGGAAATGTGCACCATCTATAGGGCTAAA 480


13549R-2.IR_full       481 GAAATCGAGGAACAAGGTGCC 501
                           |||||||||||||||||||||
13549R-2.in silico     481 GAAATCGAGGAACAAGGTGCC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_166588.1  CG13549-RA, transcript variant A (yip3), mRNA 
100   482  0  0  0  NM_079095.2  CG13549-RB, transcript variant B (yip3), mRNA 
0   0  4  3  2  NM_167107.2  CG32736-RB, transcript variant B (CG32736), mRNA 
0   0  4  3  2  NM_167106.1  CG32736-RA, transcript variant A (CG32736), mRNA 
0   0  2  3  4  NM_141239.1  CG17735-RA (CG17735), mRNA 
0   0  2  3  2  NM_141008.1  CG11456-RA (CG11456), mRNA 
0   0  2  2  2  NM_139705.1  CG10633-RA (CG10633), mRNA 
0   0  1  3  3  NM_141365.2  CG1150-RA (Osi3), mRNA 
0   0  1  3  2  NM_136594.1  CG8172-RA (CG8172), mRNA 
0   0  1  2  3  NM_057268.3  CG6944-RA (Lam), mRNA 
0   0  1  2  2  NM_168168.1  CG32396-RA (CG32396), mRNA 
0   0  1  2  2  NM_143558.1  CG2267-RA (CG2267), mRNA 
0   0  0  4  5  NM_145106.1  CG6964-RA, transcript variant A (Gug), mRNA 
0   0  0  4  5  NM_079249.2  CG6964-RB, transcript variant B (Gug), mRNA 
0   0  0  4  5  NM_176300.1  CG6964-RC, transcript variant C (Gug), mRNA 
0   0  0  4  5  NM_206303.1  CG6964-RD, transcript variant D (Gug), mRNA 
0   0  0  4  2  NM_140201.2  CG7573-RA, transcript variant A (CG7573), mRNA 
0   0  0  4  2  NM_168455.2  CG7573-RB, transcript variant B (CG7573), mRNA 
0   0  0  3  7  NM_057310.3  CG5637-RA (nos), mRNA 
0   0  0  3  6  NM_142565.2  CG6195-RA (CG6195), mRNA 
0   0  0  3  4  NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   0  0  3  3  NM_078769.2  CG11181-RA (cup), mRNA 
0   0  0  3  3  NM_137094.1  CG8394-RA (CG8394), mRNA 
0   0  0  3  3  NM_131966.1  CG6927-RA (CG6927), mRNA 
0   0  0  3  2  NM_142425.2  CG7212-RA (cdm), mRNA 
0   0  0  2  10  NM_134544.1  CG11734-RB (HERC2), mRNA 
0   0  0  2  9  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  0  2  9  NM_135312.1  CG14535-RA (CG14535), mRNA 
0   0  0  2  9  NM_001043113.1  CG33484-RC, transcript variant C (zormin), mRNA 
0   0  0  2  9  NM_206240.1  CG33484-RA, transcript variant A (zormin), mRNA 

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