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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13510R-1 |
| Symbol |
CG13510 |
Full Name |
CG13510 |
| CG No |
CG13510 |
Old CG No |
CG13510 |
| Synonyms |
CG13510 |
| Accession No (Link to NCBI) |
NM_137856.4 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACAGCAAGCAGCCTCCACCGT |
| Primer Seq. 3' |
aatctagaggtaccTCGTAGGTGCCGATGTAGGCG |
| Predicted Fragment Size |
379 |
| IR fragment full Seq |
0001 ACAGCAAGCA GCCTCCACCG TACAGTGAAC AGTCCGGATA CACTCCCGCA CAAACCTATN
0061 CAGCCCGGTG GCCCCACCCA GCCACCACTG TACCCACCGA TGCCGCAGCC ACCGCAGTCC
0121 TCAACGGTGA TCATCCAGAC CACCACAACC TCCAACCTGG TGCCCATCGG CAGTGGACCC
0181 ACCCGCATCC GCTGTCCCTC CCTGTCACGC CGAAGTACTG ACCACCGTGA AGTCCACTCC
0241 TTCCGGCAGG ACGCACTGCT gGGCCCTCAT CCTGTGCCTG TTCATCTGCT GGCCGTGCGT
0301 ATGCCTACCT TACTGCATGG ACTCCTGCCA GAATGCCAAC CACTACTGCC CCAACTGCAG
0361 CgNCTACATC GGCACCTACG A |
| in silico PCR Fragment |
0001 ACAGCAAGCA GCCTCCACCG TACAGTGAAC AGTCCGGATA CACTCCCGCA CAAACCTAT-
0061 CAGCCCGGTG GCCCCACCCA GCCACCACTG TACCCACCGA TGCCGCAGCC ACCGCAGTCC
0121 TCAACGGTGA TCATCCAGAC CACCACAACC TCCAACCTGG TGCCCATCGG CAGTGGACCC
0181 ACCCGCATCC GCTGTCCCT- CCTGTCACGC CGAAGTACTG ACCACCGTGA AGTCCACTCC
0241 TTCCGGCAGG ACGCACTGCT GGGCCCTCAT CCTGTGCCTG TTCATCTGCT GGCCGTGCGT
0301 ATGCCTACCT TACTGCATGG ACTCCTGCCA GAATGCCAAC CACTACTGCC CCAACTGCAG
0361 CGCCTACATC GGCACCTACG A |
| Assemble Data |
13510R-1.IR_full 1 ACAGCAAGCAGCCTCCACCGTACAGTGAACAGTCCGGATACACTCCCGCACAAACCTATN 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 1 ACAGCAAGCAGCCTCCACCGTACAGTGAACAGTCCGGATACACTCCCGCACAAACCTAT- 60
13510R-1.IR_full 61 CAGCCCGGTGGCCCCACCCAGCCACCACTGTACCCACCGATGCCGCAGCCACCGCAGTCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 61 CAGCCCGGTGGCCCCACCCAGCCACCACTGTACCCACCGATGCCGCAGCCACCGCAGTCC 120
13510R-1.IR_full 121 TCAACGGTGATCATCCAGACCACCACAACCTCCAACCTGGTGCCCATCGGCAGTGGACCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 121 TCAACGGTGATCATCCAGACCACCACAACCTCCAACCTGGTGCCCATCGGCAGTGGACCC 180
13510R-1.IR_full 181 ACCCGCATCCGCTGTCCCTCCCTGTCACGCCGAAGTACTGACCACCGTGAAGTCCACTCC 240
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 181 ACCCGCATCCGCTGTCCCT-CCTGTCACGCCGAAGTACTGACCACCGTGAAGTCCACTCC 240
13510R-1.IR_full 241 TTCCGGCAGGACGCACTGCTGGGCCCTCATCCTGTGCCTGTTCATCTGCTGGCCGTGCGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 241 TTCCGGCAGGACGCACTGCTGGGCCCTCATCCTGTGCCTGTTCATCTGCTGGCCGTGCGT 300
13510R-1.IR_full 301 ATGCCTACCTTACTGCATGGACTCCTGCCAGAATGCCAACCACTACTGCCCCAACTGCAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13510R-1.in silico 301 ATGCCTACCTTACTGCATGGACTCCTGCCAGAATGCCAACCACTACTGCCCCAACTGCAG 360
13510R-1.IR_full 361 CGNCTACATCGGCACCTACGA 381
|| ||||||||||||||||||
13510R-1.in silico 361 CGCCTACATCGGCACCTACGA 381
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
361 |
0 |
0 |
0 |
NM_137856.4 |
CG13510-RA (CG13510), mRNA |
| 0.27 |
1 |
2 |
4 |
2 |
NM_079259.2 |
CG4974-RA (dally), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_079161.2 |
CG13927-RA (GC), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_175965.1 |
CG11924-RC, transcript variant C (Cf2), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_175963.1 |
CG11924-RD, transcript variant D (Cf2), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078750.2 |
CG11924-RA, transcript variant A (Cf2), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_175964.1 |
CG11924-RB, transcript variant B (Cf2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_138132.1 |
CG9083-RA (CG9083), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_168240.1 |
CG32365-RA (CG32365), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_001032019.1 |
CG3992-RD, transcript variant D (srp), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_169694.1 |
CG3992-RB, transcript variant B (srp), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_169696.1 |
CG3992-RA, transcript variant A (srp), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_175969.1 |
CG33113-RF, transcript variant F (Rtnl1), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_057432.2 |
CG2762-RA (ush), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_143615.2 |
CG11518-RA (pygo), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_139938.2 |
CG7213-RA (CG7213), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141202.2 |
CG1090-RB, transcript variant B (CG1090), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164343.1 |
CG1090-RA, transcript variant A (CG1090), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_205929.1 |
CG8086-RC, transcript variant C (CG8086), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_205928.1 |
CG8086-RD, transcript variant D (CG8086), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169665.1 |
CG18519-RB, transcript variant B (CG18519), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137765.2 |
CG7975-RA (Grx-1), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_169698.1 |
CG5000-RA (msps), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_175956.1 |
CG33003-RA (CG33003), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_139400.1 |
CG8001-RA (CG8001), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_078559.2 |
CG18085-RA (sev), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_206278.1 |
CG5406-RC, transcript variant C (sif), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_079908.2 |
CG5406-RA, transcript variant A (sif), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_168126.1 |
CG5406-RB, transcript variant B (sif), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_080499.2 |
CG6898-RA (Zip3), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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